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Purified anti-LAT1/SLC7A5 Antibody

Pricing & Availability
Clone
W22208B (See other available formats)
Regulatory Status
RUO
Other Names
SLC7A5, 4F2lc, CD98Ic, MPE16, Solute Carrier Family 7 Member 5, L-Type Amino Acid Transporter 1
Isotype
Rat IgG2b, κ
Ave. Rating
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Product Citations
publications
A. W22208B_PURE_LAT1_WB_030425
Whole cell lysates (15 µg total protein) from the indicated cell lines were resolved on a 4-12% Bis-Tris gel. As a control, cell lines with no/low expression (MCF-7 and SH-SY5Y) of LAT1/SLC7A5 were included. The gel was transferred to a PVDF membrane and probed with Purified anti-LAT1/SLC7A5 (clone W22208B). Proteins were visualized by chemiluminescence detection using HRP Goat anti-rat IgG antibody (Cat. No. 405405). Direct-Blot™ HRP anti-β-Actin antibody (Cat. No. 664803) was used as a loading control. Western-Ready™ ECL Substrate Premium Kit (Cat. No. 426319) was used as a detection agent. Lane M: Molecular weight marker
  • A. W22208B_PURE_LAT1_WB_030425
    Whole cell lysates (15 µg total protein) from the indicated cell lines were resolved on a 4-12% Bis-Tris gel. As a control, cell lines with no/low expression (MCF-7 and SH-SY5Y) of LAT1/SLC7A5 were included. The gel was transferred to a PVDF membrane and probed with Purified anti-LAT1/SLC7A5 (clone W22208B). Proteins were visualized by chemiluminescence detection using HRP Goat anti-rat IgG antibody (Cat. No. 405405). Direct-Blot™ HRP anti-β-Actin antibody (Cat. No. 664803) was used as a loading control. Western-Ready™ ECL Substrate Premium Kit (Cat. No. 426319) was used as a detection agent. Lane M: Molecular weight marker
  • B. W22208B_PURE_LAT1_IHC-P_030425.
    IHC staining with anti-LAT1/SLC7A5 (clone W22208B) was performed on formalin-fixed paraffin embedded human testis tissue following antigen retrieval using Tris-EDTA pH 9.0 Antigen Retrieval Buffer (10X) (Cat. No. 422704). The tissue sections were incubated with Purified anti-LAT1/SLC7A5 (clone W22208B) (panel B and C) followed by Alexa Fluor® 647 Goat anti-rat (Cat. No. 405416) or Rat IgG2b, κ Isotype Control (Cat. No. 400602) (panel A). Nuclei were counterstained with DAPI (Cat. No. 422801) (panel A and merged in panel C). Scale Bar: 50 µm
  • C. W22208B_PURE_LAT1_ICC_030425.
    A549 cells were fixed with 4% PFA Fixation Buffer (Cat. No. 420801), permeabilized with ice cold methanol and then blocked with 5% FBS. Cells were stained with Purified anti-LAT1/SLC7A5 (clone W22208B) (panel B and C) or Rat IgG2b, κ Isotype Control (Cat. No. 400602) (panel A) followed by Alexa Fluor® 647 Goat anti-rat (Cat. No. 405416). Nuclei were counterstained with DAPI (Cat. No. 422801) (panels A and C). The images were captured on a Revvity Operetta CLS™ High Content Analysis System with a 63X objective. Scale bar: 50 µm
  • D. W22208B_PURE_LAT1_ICFC_030425
    A549 cells (positive control, filled histogram), or SH-SY5Y (low expression control, open histogram) were fixed and permeabilized using True-Phos™ Perm Buffer Set (Cat. No. 425401) and intracellularly stained with Purified anti-LAT1/SLC7A5 (clone W22208B) or Purified Rat IgG2b, κ Isotype Control (open histogram, dashed line) (Cat. No. 400602) followed by Alexa Fluor® 647 Goat anti-rat IgG (Cat. No. 405416).
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688751 25 µg 144€
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688752 100 µg 372€
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Description

L-Type Amino Acid Transporter 1 (LAT1) is crucial for transporting large neutral amino acids like leucine, isoleucine, and phenylalanine across cell membranes, playing a significant role in cellular metabolism and nutrient supply. LAT1 is highly expressed in the blood-brain barrier, where it facilitates the delivery of essential amino acids to the brain. Its regulation involves various mechanisms, including transcriptional and post-transcriptional controls, which ensure its activity aligns with cellular needs. Dysregulation of LAT1 is associated with several diseases, including cancer, where it supports tumor growth by supplying amino acids necessary for rapid cell proliferation. Additionally, LAT1 is implicated in neurological disorders like Alzheimer's and Parkinson's diseases, where it affects the transport of therapeutic agents across the blood-brain barrier.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Fragment of recombinant Human LAT1/SLC7A5
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/mL
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

WB - Quality tested
ICC, ICFC, IHC-P - Verified
Recommended Usage

Each lot of this antibody is quality control tested by western blotting. For western blotting, the suggested use of this reagent is 0.25 - 1.0 µg/mL. For immunohistochemistry on formalin-fixed paraffin-embedded tissue sections, a concentration range of 1.0 - 10.0 µg/mL is suggested. For immunohistochemistry, a concentration range of 1.25 - 10.0 µg/mL is suggested. For flow cytometric staining, the suggested use of this reagent is ≤ 0.06 µg per million cells in 100 µL volume. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

For use of this antibody in Immunocytochemistry (ICC), we recommend fixation/permeabilization using either PFA-Methanol or Methanol only. We do not recommend PFA-Triton X-100 for fixation/permeabilization.

For use of this antibody in immunohistochemistry on formalin-fixed paraffin-embedded tissues (IHC-P), we recommend antigen retrieval using either Citrate Buffer, 10X (Cat. No. 420902), or Tris-EDTA pH 9.0 Antigen Retrieval Buffer (10X) (Cat. No. 422704).

For use of this antibody in intracellular Flow Cytometry (ICFC), we recommend fixation/permeabilization using True-Phos™ Perm Buffer (Cat. No. 425401).

RRID
AB_3683498 (BioLegend Cat. No. 688751)
AB_3683498 (BioLegend Cat. No. 688752)

Antigen Details

Structure
LAT1 is a transmembrane protein that consists of 507 amino acids and has a molecular weight of approximately 55 kDa
Distribution

LAT1 is primarily localized in the plasma membrane of cells. It is highly expressed in the blood-brain barrier and the placenta, facilitating the transport of essential amino acids to the brain and fetus. LAT1 is also found in cancer cells, where it supports rapid cell growth and metabolism

Function
• Amino Acid Transport: LAT1 primarily facilitates the transport of large neutral amino acids, such as leucine, isoleucine, and phenylalanine, across cell membranes. This is crucial for cellular metabolism and protein synthesis.
• Blood-Brain Barrier: LAT1 is highly expressed in the blood-brain barrier, where it ensures the delivery of essential amino acids to the brain. This is vital for maintaining normal brain function.
• Cancer Metabolism: In cancer cells, LAT1 supports rapid cell proliferation by supplying the necessary amino acids for growth and metabolism.
Interaction
• CD98 (SLC3A2), forms a functional heterodimeric complex. This interaction is crucial for LAT1's proper localization and function on the cell membrane, facilitating the transport of large neutral amino acids.
• CLDN10, which can influence its function and contribute to disease progression, like in clear cell renal cell carcinoma (ccRCC)
Biology Area
Cancer Biomarkers, Cell Biology, Cell Proliferation and Viability, Neurodegeneration, Neuroscience, Protein Synthesis
Antigen References
  1. Zhang J, et al. 2020. Front Chem. 8: fchem.2020.564809.
  2. Ahmed HS, et al. 2024. Mol Neurobiol. s12035-024-04506-9.
  3. Zhao X, et al. 2024. Receptors. 3:474.
  4. Scalise M, et al. 2018. Front Chem. 6: fchem.2018.00243.
Regulation
• Transcriptional Control: The expression of LAT1 is regulated at the transcriptional level by various factors, including nutrient availability and cellular stress.
• Post-Transcriptional Modifications: LAT1 activity can be modulated through post-transcriptional modifications, such as phosphorylation, which can affect its stability and function.
• Interaction with CD98: LAT1 forms a heterodimer with CD98 (SLC3A2), which is essential for its proper localization and function on the cell membrane
Gene ID
8140 View all products for this Gene ID
UniProt
View information about LAT1/SLC7A5 on UniProt.org

Related FAQs

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Go To Top Version: 1    Revision Date: 03.04.2025

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
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