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Purified anti-mouse CD107a (LAMP-1) Antibody

Pricing & Availability
Clone
1D4B (See other available formats)
Regulatory Status
RUO
Other Names
Lysosome-associated membrane glycoprotein 1, lysosomal membrane glycoprotein 1, CD107 antigen-like family member A, lysosome-associated membrane protein 1
Isotype
Rat IgG2a, κ
Ave. Rating
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Product Citations
publications
A_1D4B_Purified_083107
Thioglycollate-elicited BALB/c mouse peritoneal macrophages stained with purified 1D4B, followed by anti-rat IgG FITC
  • A_1D4B_Purified_083107
    Thioglycollate-elicited BALB/c mouse peritoneal macrophages stained with purified 1D4B, followed by anti-rat IgG FITC
  • B_1D4B_PURE_CD107a_LAMP1_WB_022708
    Western blot of purified anti-LAMP-1 antibody (clone 1D4B). Lane 1: Molecular weight marker; Lane 2: 20 µg of Hela cell lysate; Lane 3: 20 µg of NIH3T3 cell lysate; Lane 4: 20 µg of human brain lysate; Lane 5: 20 µg of mouse brain lysates; Lane 6: 10 ng human recombinant LAMP-1. The blot was incubated with 1 µg/ml of the primary antibody overnight at 4°C, followed by incubation with HRP-labeled goat anti-rat IgG (Cat. No. 405405). Anti-β-actin antibody was used as the loading control (Cat. No. 643807). Enhanced chemiluminescence was used as the detection system.
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121601 50 µg 67€
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121602 200 µg 151€
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Description

CD107a, also known as Lysosome-Associated Membrane Protein 1 (LAMP-1) or LGP-120, is a 110-140 kD type I membrane glycoprotein. Mature CD107a is heavily glycosylated from a 40 kD core protein. This molecule is located on the luminal side of lysosomes. Upon activation, CD107a is transferred to the cell membrane surface of activated platelets, activated lymphocytes, macrophages, epithelial cells, endothelial cells, and some tumor cells. CD107a has been suggested to play a role in the protection of lysosomal membrane from lysosomal hydrolases which is involved in cell adhesion and regulation of tumor metastasis, and mediates autoimmune disease progression. CD107a is a ligand for galaptin and E-selectin. Surface expression of LAMP-1 has been shown to correlate with CD8+ T cell and NK cell cytotoxicity.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
This monoclonal antibody was raised against NIH/3T3 mouse embryonic fibroblast tissue culture cell membranes. It has been mapped to the N-terminus of LAMP-1.
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

FC - Quality tested
WB - Verified
ICC, IP - Reported in the literature, not verified in house
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 µg per million cells in 100 µl volume. For Western blotting, the suggested use of this reagent is 1.0 - 5.0 µg per ml. It is recommended that the reagent be titrated for optimal performance for each application.

Product Citations
  1. Zhou D, et al. 2018. Oncotarget. 9:5184. PubMed
  2. Miyatake Y, et al. 2018. J Immunol. 201:3051. PubMed
  3. Morisaki Y, et al. 2016. Sci Rep. 6:27354. PubMed
  4. Roblek M, et al. 2022. Front Oncol. 12:777634. PubMed
  5. Tersteegen A, et al. 2021. Infect Immun. 89:. PubMed
  6. Yan J, et al. 2020. Cancer Discov. 10:124. PubMed
  7. Blériot C, et al. 2020. Methods Mol Biol. 2164:87. PubMed
  8. Kim S, et al. 2023. Sci Rep. 13:5665. PubMed
  9. Zeng J, et al. 2023. Nat Commun. 14:2573. PubMed
  10. Wong AYW, et al. 2018. Front Immunol. 9:210. PubMed
  11. Warburg G, et al. 2014. J Cell Biol. 204:423. PubMed
  12. Steptoe R, et al. 2007. J Immunol . 178:2094. PubMed
  13. Reichenbach N, et al. 2018. J Exp Med. 215:1649. PubMed
  14. Hasegawa J, et al. 2016. EMBO J. 35: 1853 - 1867. PubMed
  15. Tan L, et al. 2017. Int J Nanomedicine. 12:3095. PubMed
  16. Reichenbach N, et al. 2019. EMBO Mol Med. 11:. PubMed
  17. Yan J, et al. 2020. Cancer Immunol Res. 8:356. PubMed
  18. Stacey MA, et al. 2017. J Clin Invest. 127:1463. PubMed
  19. Morioka S, et al. 2018. J Cell Sci. 131. PubMed
  20. Takemasu S, et al. 2019. J Biochem. 166:175. PubMed
  21. Hsiao CC, et al. 2021. Cells. 10:. PubMed
  22. Bussi C, et al. 2018. J Cell Sci. 131. PubMed
  23. Daley D, et al. 2017. Nat Med. 23:556. PubMed
  24. Blriot C, et al. 2021. Immunity. :. PubMed
  25. Tan CL, et al. 2018. Immunohorizons. 0.248611111. PubMed
  26. D'Agostino M, et al. 2011. J Cell Sci. 124:3545. PubMed
  27. Wagner AK, et al. 2017. Nat Commun. 8:15627. PubMed
  28. Stelekati E, et al. 2018. Cell Rep. 2.445833333. PubMed
  29. Stykel MG, et al. 2021. Cell Reports. 35(6):109099. PubMed
  30. Maier B, et al. 2020. Nature. 580:257. PubMed
RRID
AB_572020 (BioLegend Cat. No. 121601)
AB_572020 (BioLegend Cat. No. 121602)

Antigen Details

Structure
LAMP-1 is a 406 amino acid protein with a molecular mass of 44 kD.
Gene ID
16783 View all products for this Gene ID
UniProt
View information about CD107a on UniProt.org

Related FAQs

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Go To Top Version: 3    Revision Date: 02.27.2018

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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