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Purified anti-mouse CD154 Recombinant Antibody

Pricing & Availability
Clone
QA17A53 (See other available formats)
Regulatory Status
RUO
Other Names
CD40 ligand (CD40L), gp39, T-BAM, TRAP, Ly-62, TNFSF5
Isotype
Rat IgG1, κ
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Product Citations
publications
QA17A53_Purified_CD154_Antibody_1_022420
Enriched C57BL/6 mouse splenic T cells were stimulated with PMA + ionomycin (Cell Activation Cocktail w/o Brefeldin A) for 4 hours then stained with anti-mouse CD4 APC and CD154 (clone QA17A53) purified followed by anti-rat IgG1, κ PE secondary antibody (left) or rat IgG1, κ purified isotype control followed by anti-rat IgG1 PE secondary antibody (right).
  • QA17A53_Purified_CD154_Antibody_1_022420
    Enriched C57BL/6 mouse splenic T cells were stimulated with PMA + ionomycin (Cell Activation Cocktail w/o Brefeldin A) for 4 hours then stained with anti-mouse CD4 APC and CD154 (clone QA17A53) purified followed by anti-rat IgG1, κ PE secondary antibody (left) or rat IgG1, κ purified isotype control followed by anti-rat IgG1 PE secondary antibody (right).
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158102 500 µg 221€
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Description

CD154 is a 39 kD TNF superfamily member also known as CD40 ligand, gp39, T-BAM, TRAP, and Ly-62. CD154 is an accessory molecule expressed predominantly on activated CD4+ lymphocytes that bind CD40. CD154 plays an important role in T-B cell costimulation.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Mouse
Antibody Type
Recombinant
Host Species
Rat
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/mL
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 µg per million cells in 100 µL volume. It is recommended that the reagent be titrated for optimal performance for each application.

RRID
AB_2832561 (BioLegend Cat. No. 158102)

Antigen Details

Structure
TNF superfamily, 39kD
Distribution

Activated CD4+ T-cells

Function
T-B cell costimulation
Interaction
T-cells, Tregs
Ligand/Receptor
CD40
Cell Type
T cells, Tregs
Biology Area
Costimulatory Molecules, Immunology
Molecular Family
Adhesion Molecules, CD Molecules
Antigen References
  1. Barclay A, et al. 1997. The Leukocyte Antigen FactsBook Academic Press.
  2. Noelle RJ, et al. 1992. Proc. Natl. Acad. Sci. USA 89:6550.
  3. Banchereau J, et al. 1994. Annu. Rev. Immunol. 12:881.
  4. Clark EA and Ledbetter JA. 1986. Proc. Natl. Acad. Sci. USA 83:4494.
Gene ID
21947 View all products for this Gene ID
UniProt
View information about CD154 on UniProt.org
Go To Top Version: 1    Revision Date: 02.24.2020

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

BioLegend, the BioLegend logo, and all other trademarks are property of BioLegend, Inc. or their respective owners, and all rights are reserved.

 

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Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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