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Purified anti-mouse CD16/32 Antibody

Pricing & Availability
Clone
93 (See other available formats)
Regulatory Status
RUO
Other Names
Fcγ R III/II, Ly-17
Isotype
Rat IgG2a, λ
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Product Citations
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93_purified_051906
C57BL/6 mouse splenocytes stained with purified 93, followed by anti-rat IgGs FITC
  • 93_purified_051906
    C57BL/6 mouse splenocytes stained with purified 93, followed by anti-rat IgGs FITC
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101301 50 µg 29€
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101302 500 µg 92€
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Description

CD16 is low affinity IgG Fc receptor III (FcR III) and CD32 is FcR II. CD16/CD32 are expressed on B cells, monocytes/macrophages, NK cells, granulocytes, mast cells, and dendritic cells. The Fc receptors bind antibody-antigen immune complexes and mediate adaptive immune responses.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Sorted pre-B cells
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/ml
Storage & Handling
The CD16/32 antibody solution should be stored undiluted between 2°C and 8°C.
Application

FC - Quality tested
IP, Block - Reported in the literature, not verified in house
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 1.0 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

Clone 93 can be used for blocking of CD16/CD32 interactions with the Fc domain of immunoglobulins, but is not the same clone as 2.4G2.

The 93 mAb is specific to the common epitope of CD16/CD32. Additional reported applications (for the relevant formats) include: immunoprecipitation1 and blocking of Fc-mediated reactions in functional studies2,4,23. It is useful for blocking non-specific binding of immunoglobulin to Fc receptors. For blocking of Fc receptors in flow cytometric analysis, pre-incubate the cells with purified anti-CD16/CD32 antibody (=1.0 µg per 106 cells in 100 µL volume) for 5-10 minutes on ice prior to immunostaining. For highly sensitive assays, we recommend Ultra-LEAF™ purified antibody (Cat. No. 101330) (Endotoxin <0.01 EU/µg, Azide-Free, 0.2 µm filtered).

Application References
  1. Personal communication (IP)
  2. Oliver AM, et al. 1999. Hybridoma 18:113. (Block)
  3. Brummel R and Lenert P. 2005. J. Immunol. 174:2429.
  4. Terrazas LI, et al. 2005. Int. J. Parasitol. 35:1349. (Block)
  5. Clements JL, et al. 2006. J. Immunol. 177:905.
  6. Mohamed W, et al. 2010. Infect Immun. 78:3306. PubMed
  7. Ouchi T, et al. 2011. J. Exp Med. 208:2607. PubMed
  8. Kmieciak M, et al. 2011. J. Vis. Exp. 47:2381. PubMed
  9. Yamazaki S, et al. 2012. PLoS One. 7:e51665. PubMed
  10. Li J, et al. 2012. Arthritis Rheum. 64:1098. PubMed
  11. Azuma M, et al. 2012. Oncoimmunology. 1:581. PubMed
  12. Koon HW, et al. 2013. J. Vis. Exp. 68:4208. PubMed
  13. Hegde VL, et al. 2013. J Biol Chem. 288:36810. PubMed
  14. Huang J, et al. 2013. J. Immunol Methods. 387:254. PubMed
  15. Dutow P, et al. 2014. J Infect Dis. PubMed
  16. Fan Y, et al. 2014. J Exp Med. 211:313. PubMed
  17. Huang HN, et al. 2014. Antimicrob Agents Chemother. 58:1538. PubMed
  18. Takei S, et al. 2014. Vaccine. 32:3066. PubMed
  19. Richardson ML, et al. 2014. PLoS Negl Trop Dis. 8:2825. PubMed
  20. Cekanaviciute E, et al. 2014. J Immunol. 193:139. PubMed
  21. Kimura T, et al. 2014. Int Immunol. 26:697. PubMed
  22. Everad A, et al. 2014. Nat Commun. 5:5648. PubMed
  23. Cenci E, et al. 2006. J. Leuko. Biol. 79(1):40-5. (Block)
Product Citations
  1. Sade-Feldman M,et al. 2017. Nat Commun.. 10.1038/s41467-017-01062-w. PubMed
  2. Michela Miani et al. 2018. Cell metabolism. 28(4):557-572 . PubMed
  3. Gan T, et al. 2019. Front Microbiol. 9:3174. PubMed
  4. Furuhashi K, et al. 2017. Immunology. 151:227. PubMed
  5. Fomin ME, et al. 2017. Cell Med. 0.45625. PubMed
  6. Tomida S, et al. 2019. Sci Rep. 9:10751. PubMed
  7. Filippini D, et al. 2019. Sci Rep. 9:12286. PubMed
  8. Flamar AL, et al. 2020. Immunity. 52(4):606-619.e6.. PubMed
  9. Lee J, et al. 2015. Hum Reprod. 10.1093/humrep/dev222. PubMed
  10. Bussières-Marmen S, et al. 2017. Cell Mol Immunol. 10.1038/cmi.2016.72. PubMed
  11. Chida J, et al. 2020. PLoS Pathog. 16:e1008823. PubMed
  12. Iyer S, et al. 2021. Cancer Discov. 0.725. PubMed
  13. Park E, et al. 2021. Journal of Neuroscience Research. . PubMed
  14. Gabitova-Cornell L, et al. 2020. Cancer Cell. 38(4):567-583.e11. PubMed
  15. Kosti P, et al. 2021. Cell Reports Medicine. 2(4):100227. PubMed
  16. Barkaway A, et al. 2021. Immunity. . PubMed
  17. Shah D, et al. 2021. Oncoimmunology. 10:1939601. PubMed
  18. Hutton C, et al. 2021. Cancer Cell. 39:1227. PubMed
  19. Foster A, et al. 2021. EBioMedicine. 71:103571. PubMed
  20. Kiepura A, et al. 2021. Int J Mol Sci. 22:. PubMed
  21. Rajagopalan A, et al. 2021. Cell Rep Methods. 1:. PubMed
  22. Guo H, et al. 2020. Curr Protoc Immunol. 131:e107. PubMed
  23. Van Braeckel-Budimir N, et al. 2021. J Immunother Cancer. 9:. PubMed
  24. Wang Z, et al. 2022. Proc Natl Acad Sci U S A. 119:. PubMed
  25. Jebbawi F, et al. 2021. Parasite Immunol. 43:e12834. PubMed
  26. Thomas R, et al. 2021. Immunology. 163:478. PubMed
  27. Herz J, et al. 2021. Neuron. 109:3609. PubMed
  28. Li H, et al. 2022. Cancer Cell. 40:36. PubMed
  29. Glynn RA, et al. 2022. J Immunol. 208:371. PubMed
  30. Kozuki S, et al. 2022. Genes Cells. 27:192. PubMed
  31. Bettke JA, et al. 2022. Infect Immun. 90:e0007022. PubMed
  32. Guttman O, et al. 2022. J Cell Biol. 221: . PubMed
  33. Miyao T, et al. 2022. Elife. 11: . PubMed
  34. Yin K, et al. 2022. Genes Dev. 36:533. PubMed
  35. Malhi H, et al. 2022. Cell Rep Med. 3:100658. PubMed
  36. Zhang Y, et al. 2022. Vaccines (Basel). 10: . PubMed
  37. Sugimoto S, et al. 2022. Nat Metab. 4:775. PubMed
  38. Hong Y, et al. 2022. J Leukoc Biol. 112:425. PubMed
  39. Que W, et al. 2022. Sci Adv. 8:eabo4413. PubMed
  40. Li X, et al. 2022. Cell Mol Gastroenterol Hepatol. 14:1077. PubMed
  41. Akalu YT, et al. 2022. Elife. 11: . PubMed
  42. Zhang X, et al. 2022. EBioMedicine. 84:104275. PubMed
  43. Bottermann K, et al. 2022. Basic Res Cardiol. 117:48. PubMed
  44. Hao J, et al. 2022. Cell Rep. 41:111804. PubMed
  45. McNamara NB, et al. 2023. Nature. 613:120. PubMed
  46. Miró L, et al. 2022. Cancers (Basel). 14: . PubMed
  47. Liu P, et al. 2023. Clin Immunol. 246:109212. PubMed
  48. Ullah I, et al. 2023. Cell Rep Med. 4:100893. PubMed
  49. Al-Saafeen BH, et al. 2023. Front Immunol. 13:1017780. PubMed
  50. Cousin N, et al. 2022. Cells. 12: . PubMed
  51. Peng Q, et al. 2023. EMBO Rep. 24:e56034. PubMed
  52. Tichet M, et al. 2023. Immunity. 56:162. PubMed
  53. Zhou H, et al. 2023. Front Immunol. 13:1059687. PubMed
  54. Mandour MF, et al. 2023. Front Med (Lausanne). 9:1057252. PubMed
  55. Kemna J, et al. 2023. Nat Immunol. 24:414. PubMed
  56. Novaj A, et al. 2023. Int J Mol Sci. 24: . PubMed
  57. Ishikawa T, et al. 2023. Bio Protoc. 13:e4588. PubMed
  58. Liu X, et al. 2022. Pain. 163:508. PubMed
  59. Zhang M, et al. 2022. Mol Imaging Biol. 24:280. PubMed
  60. Zhang W, et al. 2022. Dev Cell. 57:329. PubMed
  61. Kodali S, et al. 2022. J Immunol. 208:1085. PubMed
  62. Chen K, et al. 2022. Adv Sci (Weinh). 9:e2104106. PubMed
  63. Zhang H, et al. 2022. Nat Cancer. 3:808. PubMed
  64. Fang Y, et al. 2022. Sci Immunol. 7:eabp9962. PubMed
  65. Koch MS, et al. 2022. Mol Ther Oncolytics. 26:275. PubMed
  66. Harapas CR, et al. 2022. Sci Immunol. 7:eabi4611. PubMed
  67. Ding P, et al. 2022. Elife. 11:. PubMed
  68. Deák P, et al. 2022. Cell Rep. 41:111563. PubMed
  69. Zhong J, et al. 2022. J Adv Res. 41:145. PubMed
  70. Melo-Silva CR, et al. 2022. Cell Rep. 41:111676. PubMed
  71. Li Y, et al. 2023. Adv Mater. 35:e2208923. PubMed
  72. Wu Q, et al. 2023. Front Immunol. 13:1081719. PubMed
  73. Li L, et al. 2023. iScience. 26:106095. PubMed
  74. Torrente D, et al. 2023. J Neuroinflammation. 20:56. PubMed
  75. Bai X, et al. 2023. Nat Commun. 14:1247. PubMed
  76. Rocca Y, et al. 2023. Methods Mol Biol. 2618:187. PubMed
  77. Dang YZ, et al. 2023. Neoplasma. 70:123. PubMed
  78. Senatus L, et al. 2023. Commun Biol. 6:280. PubMed
  79. Kudo T, et al. 2023. iScience. 26:106342. PubMed
  80. Liu KY, et al. 2023. Environ Health Perspect. 131:37014. PubMed
  81. Zhang YN, et al. 2023. Nat Commun. 14:1985. PubMed
  82. Iguchi A, et al. 2023. iScience. 26:106375. PubMed
  83. Feng Y, et al. 2023. J Transl Med. 21:290. PubMed
  84. Ji Y, et al. 2023. Nat Cell Biol. 25:726. PubMed
  85. Liu JC, et al. 2023. Int J Mol Sci. 24:. PubMed
  86. Petenkova A, et al. 2023. Nat Commun. 14:2761. PubMed
  87. Xu J, et al. 2023. Discov Oncol. 14:68. PubMed
  88. An R, et al. 2022. Cell Insight. 1:100047. PubMed
  89. Wang K, et al. 2023. Nat Commun. 14:2950. PubMed
  90. Amirova KM, et al. 2023. Int J Mol Sci. 24:. PubMed
  91. Li M, et al. 2023. BMC Cancer. 23:497. PubMed
  92. Mawambo G, et al. 2023. J Neuroinflammation. 20:145. PubMed
  93. Akasaka H, et al. 2023. JCI Insight. 8:. PubMed
  94. Xiang G, et al. 2022. Cell Death Dis. 13:451. PubMed
  95. Nederlof R, et al. 2022. Front Immunol. 13:908023. PubMed
  96. Cerrato G, et al. 2021. Cell Death Differ. 28:1733. PubMed
  97. Da Mesquita S, et al. 2021. Nature. 593:255. PubMed
  98. Liu C, et al. 2022. Nat Nanotechnol. 17:531. PubMed
  99. MacLean M, et al. 2022. Commun Biol. 5:177. PubMed
  100. Effern M, et al. 2022. STAR Protoc. 3:101038. PubMed
  101. Rødahl I, et al. 2021. STAR Protoc. 2:100842. PubMed
  102. Correa S, et al. 2020. ACS Nano. 14:2224. PubMed
  103. Wong Fok Lung T, et al. 2020. Nat Microbiol. 141:5. PubMed
  104. Qian Y, et al. 2021. Cell Reports. 36(8):109602. PubMed
  105. Benitez AA, et al. 2020. Commun Biol. 0.371527778. PubMed
  106. Castoldi Â, et al. 2020. Nat Commun. 3.310416667. PubMed
  107. Mevel R, et al. 2020. eLife. 9:00. PubMed
  108. Fan Y, et al. 2014. J Exp Med. 211:313. PubMed
  109. Berry CT, et al. 2020. Cell Reports. 31(2):107474.. PubMed
  110. Rendeiro AF, et al. 2020. Nat Commun. 11:577. PubMed
  111. Biljes D, et al. 2017. EXCLI J. 0.86875. PubMed
  112. Zhang R, et al. 2019. Cell Rep. 28:2647. PubMed
  113. McGill BE et al. 2018. The Journal of Neuroscience. 38(1):200-219 . PubMed
  114. Zou B, et al. 2017. PLoS Pathogens. 13(6):e1006436. PubMed
  115. Kitagawa S, et al. 2022. NPJ Vaccines. 7:115. PubMed
  116. Golden GJ, et al. 2021. MBio. 12:e0118121. PubMed
  117. Yu Y, et al. 2022. iScience. 25:105004. PubMed
  118. Huang Y, et al. 2021. Nat Immunol. 22:586. PubMed
  119. Tan H,et al. 2017. Mucosal Immunol. . 10.1038/mi.2017.89. PubMed
  120. Clarke F,et al. 2017. PLoS One. 10.1371/journal.pone.0186625. PubMed
  121. Postat J et al. 2018. Immunity. 49(4):654-665 . PubMed
  122. Minutti CM, et al. 2019. Immunity. 50:645. PubMed
  123. Farsakoglu Y et al. 2019. Cell reports. 26(9):2307-2315 . PubMed
  124. Takeda Y, et al. 2018. Innate Immun. 24:323. PubMed
  125. Goel S, et al. 2017. Nature. 548:471. PubMed
  126. Matsui S, et al. 2018. Am J Pathol. 188:2059. PubMed
  127. Bhatia D, et al. 2019. JCI Insight. 4:e132826. PubMed
  128. Noh JR, et al. 2018. Sci Rep. 8:15222. PubMed
  129. Flores M, et al. 2008. FASEB J. 3.459027778. PubMed
  130. Tong J, et al. 2011. J Immunol. 186:3686. PubMed
  131. Weng L, et al. 2016. Sci Rep. 6:38632. PubMed
  132. Browning D, et al. 2017. Gene Ther. 10.1038/gt.2016.88. PubMed
  133. Morris MG, et al. 2020. Comp Med. 70:336. PubMed
  134. Sano T, et al. 2021. Cell Reports. 36:109608. PubMed
  135. Martos-Rus C, et al. 2021. Sci Rep. 11:2974. PubMed
  136. Liu P, et al. 2021. Front Med (Lausanne). 8:625130. PubMed
  137. Song J, et al. 2021. J Am Heart Assoc. 10:e017329. PubMed
  138. Jiang W, et al. 2021. Oncol Lett. 22:625. PubMed
  139. Khamissi FZ, et al. 2022. Sci Adv. 8:eabm5900. PubMed
  140. Chen L, et al. 2022. Mol Ther Oncolytics. 24:522. PubMed
  141. Almeida AS, et al. 2021. Bio Protoc. 11:e4012. PubMed
  142. Feng J, et al. 2021. iScience. 24:103004. PubMed
  143. Wu L, et al. 2022. Front Immunol. 13:860761. PubMed
  144. Bauer KM, et al. 2022. JCI Insight. 7:. PubMed
  145. Roberto Raúl SG, et al. 2022. Breast Cancer (Auckl). 16:11782234211065154. PubMed
  146. Matthias J, et al. 2020. J Clin Invest. 130:4587. PubMed
  147. Menzel L, et al. 2021. Cell Rep. 37:109878. PubMed
  148. Liang F, et al. 2021. Front Immunol. 12:658715. PubMed
  149. Jenull S, et al. 2021. Cell Reports. 36(3):109406. PubMed
  150. Clark AL, et al. 2021. Diabetes Obesity and Metabolism. :. PubMed
  151. Stahnke S, et al. 2021. Current Biology. 31(10):2051-2064.e8. PubMed
  152. Yang SB, et al. 2021. Cancers (Basel). 13:00. PubMed
  153. Elisabeth Schrumpf, Xiaojun Jiang 2017. Physiol Rep. 10.14814/phy2.13117. PubMed
  154. Koon H, et al. 2012. J Vis Exp. 68: 4208. PubMed
  155. Azuma M, et al. 2012. Oncoimmunology. 1:581. PubMed
  156. Yamazaki S, et al. 2012. PLoS One. 7:e51665. PubMed
  157. Yang W, et al. 2020. Nat Commun. 3.553472222. PubMed
  158. Hays C, et al. 2020. eLife. 8:e48772.. PubMed
  159. Li Y, et al. 2020. Cell Rep. 30:1753. PubMed
  160. Gawden‐Bone CM et al. 2018. Immunity. 49(3):427-437 . PubMed
  161. Tyagi AM et al. 2018. Immunity. 49(6):1116-1131 . PubMed
  162. Oyarce C, et al. 2018. Front Immunol. 8:1794. PubMed
  163. Diao L, et al. 2022. iScience. 25:105511. PubMed
  164. Zhang Y, et al. 2022. Cancers (Basel). 14:. PubMed
  165. Mirshahi F, et al. 2022. Cell Rep. 38:110454. PubMed
  166. Sun SJ, et al. 2022. Commun Biol. 5:867. PubMed
  167. Chen X, et al. 2021. Cell Death Differ. 28:1237. PubMed
  168. Nakagawa S et al. 2017. Cell host & microbe. 22(5):667-677 . PubMed
  169. Mann M, et al. 2018. Cell Rep. 25:2992. PubMed
  170. Fiala GJ, et al. 2019. J Immunol. 203:569. PubMed
  171. Dave K et al. 2017. eLife. 6 pii: e23382. PubMed
  172. Kitashima DY, et al. 2018. EBioMedicine. 27:293. PubMed
  173. Li Y, et al. 2018. J Immunol. 201:2910. PubMed
  174. Carlson P, et al. 2019. Nat Cell Biol. 21:238. PubMed
  175. Wu J, et al. 2020. Sci Adv. 6:eaba3458. PubMed
  176. Guan H, et al. 2012. PLoS One. 7:e35650. PubMed
  177. Hazama D, et al. 2020. Cell Chemical Biology. 27(9):1181-1191.e7. PubMed
  178. Shemer A, et al. 2020. Immunity. 53(5):1033-1049.e7. PubMed
  179. Schiller M, et al. 2021. Immunity. 54(5):1022-1036.e8. PubMed
  180. Okuniewska M, et al. 2021. Cell Reports. 36(2):109368. PubMed
  181. Zhao B, et al. 2021. Cell Reports. 36(3):109401. PubMed
  182. Webster HC, et al. 2020. J Immunol Methods. 112702:477. PubMed
  183. Godbersen-Palmer C, et al. 2020. J Immunol. 204:2973. PubMed
  184. Yang C, et al. 2020. J Control Release. 326:324. PubMed
  185. Liu D, et al. 2021. JCI Insight. 6:. PubMed
  186. Armaka M, et al. 2022. Genome Med. 14:78. PubMed
  187. Hoffman D, et al. 2021. Immunity. 54:2712. PubMed
  188. Shen H, et al. 2022. Nat Commun. 13:6030. PubMed
  189. Ma J, et al. 2021. Aging (Albany NY). 13:7211. PubMed
  190. Yu M, et al. 2021. J Clin Invest. 131:. PubMed
  191. Cousin N, et al. 2021. Cancer Res. 81:4133. PubMed
  192. Haugh KA, et al. 2021. eLife. 0.416666666666667. PubMed
  193. Tal MC, et al. 2020. MBio. 11:00. PubMed
  194. Zhang X, et al. 2020. J Immunol. 205:1743. PubMed
  195. Kovacs L, et al. 2020. PLoS One. 15:e0240455. PubMed
  196. Vackova J, et al. 2020. Int J Mol Sci. 21:00. PubMed
  197. Terrazas LI, et al. 2005. Int J Parasit. 35:1349. PubMed
  198. Bhattacherjee A, et al. 2019. Commun Biol. 2:450. PubMed
  199. Lieschke S, et al. 2019. Cells. 0.420833333. PubMed
  200. Sereni L, et al. 2018. J Allergy Clin Immunol. 142:1272. PubMed
  201. Fransen F, et al. 2017. Front Immunol. 1.295138889. PubMed
  202. Nakamura‐Ishizu A et al. 2018. Cell reports. 25(7):1772-1785 . PubMed
  203. Wlodarska M et al. 2017. Cell host & microbe. 22(1):25-37 . PubMed
  204. Wang Y, et al. 2022. Front Immunol. 13:767630. PubMed
  205. Wang L, et al. 2017. Front Immunol.. 10.3389/fimmu.2017.01754. PubMed
  206. Fiaturi N, et al. 2018. J Cell Commun Signal. 314:L127. PubMed
  207. Barajas‐Mora EM et al. 2018. Molecular cell. 73(1):48-60 . PubMed
  208. Fujita Y et al. 2018. Cell reports. 24(12):3296-3311 . PubMed
  209. Uchil PD et al. 2018. Cell host & microbe. 25(1):87-100 . PubMed
  210. Koliaraki V et al. 2019. Cell reports. 26(3):536-545 . PubMed
  211. Finn J et al. 2019. Cell reports. 26(11):2942-2954 . PubMed
  212. Barger SR, et al. 2019. Nat Commun. 10:1249. PubMed
  213. Shimokawa C et al. 2017. Immunity. 46(5):863-874 . PubMed
  214. Caronni N, et al. 2018. Cancer Res. 78:1685. PubMed
  215. Weiner AI, et al. 2019. NPJ Regen Med. 4:17. PubMed
  216. Russell SL, et al. 2019. Cell Rep. 29:3564. PubMed
  217. Khaw YM, et al. 2020. J Neuroinflammation. 17:49. PubMed
  218. Chen C, et al. 2019. Cell Rep. 29:4200. PubMed
  219. Damle SR, et al. 2018. Allergy. 73:125. PubMed
  220. Cheng XS, et al. 2020. J Cell Mol Med. 24:8391. PubMed
  221. Kohn M, et al. 2021. Front Immunol. 580594:12. PubMed
  222. Liang Z, et al. 2020. Pain. 161:2089. PubMed
  223. Gui J, et al. 2020. Nat Cancer. 1:603. PubMed
  224. Hore ZL, et al. 2021. Wellcome Open Res. 6:68. PubMed
  225. Sheller-Miller S, et al. 2021. Sci Adv. 7:. PubMed
  226. Mao W, et al. 2021. Int J Mol Sci. 22:. PubMed
  227. Dang Y, et al. 2022. Cancer Cell Int. 22:88. PubMed
  228. Almeida AS, et al. 2021. Bio Protoc. 11:e4012. PubMed
  229. Cong M, et al. 2021. Methods Mol Biol. 2388:59. PubMed
  230. Menzel L, et al. 2022. STAR Protoc. 3:101267. PubMed
  231. Escudero-Lara A, et al. 2022. Front Behav Neurosci. 16:946975. PubMed
  232. Korangath P, et al. 2020. Bio Protoc. 10:e3822. PubMed
  233. Flommersfeld S, et al. 2021. Immunity. :. PubMed
  234. Wong HS, et al. 2021. Cell. . PubMed
  235. Kusuyama J, et al. 2021. Cell Metabolism. 33(5):939-956.e8. PubMed
  236. Alshetaiwi H, et al. 2020. Sci Immunol. 5:00. PubMed
  237. Gereke M, et al. 2012. J Vis Exp. 70: 4322. PubMed
  238. MacConmara M, et al. 2011. J Leukoc Biol. 89:137. PubMed
  239. Cassidy LD, et al. 2018. Autophagy. 1.455555556. PubMed
  240. Fransen F, et al. 2017. Front Immunol. 8:154. PubMed
  241. Yang SH, et al. 2017. Front Immunol. 8:1192. PubMed
  242. Lagares D, et al. 2017. Sci Transl Med. 9:eaal3765. PubMed
  243. McLaughlin PA et al. 2019. PLoS Pathog. 15(7):e1007847 . PubMed
  244. Mrdjen D et al. 2018. Immunity. 48(2):380-395 . PubMed
  245. Go MJ, et al. 2018. Mol Med Rep. 13:e0194423. PubMed
  246. McGrath-Morrow S, et al. 2017. Cytokine. 10.1016/j.cyto.2017.06.002. PubMed
  247. Lopez DA, et al. 2022. Cell Rep. 41:111677. PubMed
  248. Ruf B, et al. 2021. Cancer Immunol Res. 9:1024. PubMed
  249. Muñoz NM, et al. 2022. Sci Rep. 12:14449. PubMed
  250. Hu G, et al. 2022. Cell Death Dis. 13:640. PubMed
  251. Sun Y, et al. 2022. Nat Commun. 13:3916. PubMed
  252. Csóka B, et al. 2018. JCI Insight. 38:200. PubMed
  253. Proto JD et al. 2018. Immunity. 49(4):666-677 . PubMed
  254. Rashidi M, et al. 2019. J Immunol. 203:736. PubMed
  255. Deng M, et al. 2020. Nat Commun. 11:2193. PubMed
  256. Hashimoto M, et al. 2019. FASEB J. 33:8069. PubMed
  257. Everard A, et al. 2014. Nat Commun. 5:5648. PubMed
  258. Francis M, et al. 2017. Toxicol Sci. 155(1):182-195. PubMed
  259. Hu J, et al. 2020. Theranostics. 7.154166667. PubMed
  260. Baasch S, et al. 2021. Cell. . PubMed
  261. Sutherland TE, et al. 2021. Frontiers in Immunology. 12:715209. PubMed
  262. Zheng J, et al. 2020. bioRxiv. . PubMed
  263. Imani J, et al. 2021. JCI Insight. 6:. PubMed
  264. Dang Y, et al. 2021. J Cancer. 12:7158. PubMed
  265. Du Y, et al. 2022. Nat Commun. 13:231. PubMed
  266. Pishesha N, et al. 2021. Proc Natl Acad Sci U S A. 118:. PubMed
  267. Chin AL, et al. 2021. Nat Commun. 12:5138. PubMed
  268. Hong CH, et al. 2021. Cell Mol Gastroenterol Hepatol. 13:925. PubMed
  269. Zeng Q, et al. 2022. Front Immunol. 13:740805. PubMed
  270. Welte T, et al. 2021. iScience. 24:103349. PubMed
  271. Baier FA, et al. 2021. Cell Mol Gastroenterol Hepatol. 12:745. PubMed
  272. Li Y, et al. 2021. Sci Transl Med. 13:. PubMed
  273. Dobosz E, et al. 2021. Dis Model Mech. :14. PubMed
  274. Hubbard JJ, et al. 2020. J Exp Med. :217. PubMed
  275. Xu S, et al. 2021. Immunity. . PubMed
  276. Tacconi C, et al. 2021. Cell Reports. 35(2):108993. PubMed
  277. Dieterich LC, et al. 2020. Angiogenesis. 1.24375. PubMed
  278. Duvigneau S, et al. 2016. Sci Rep. 6:37045. PubMed
  279. Siggs O, et al. 2015. Proc Natl Acad Sci U S A. 112: E5706 - E5714. PubMed
  280. Moon H, et al. 2020. Nat Commun. 4.270138889. PubMed
  281. Kulkarni U, et al. 2019. Mucosal Immunol. 12:545. PubMed
  282. Tsubaki T, et al. 2018. Oncotarget. 9:11209. PubMed
  283. Qi S et al. 2016. eLife. 5 pii: e14756. PubMed
  284. Roy K, et al. 2019. Immunity. 50:616. PubMed
  285. Chen Y, et al. 2022. Front Immunol. 13:835690. PubMed
  286. Lin C, et al. 2022. Nat Commun. 13:6869. PubMed
  287. De Santi C, et al. 2022. Mol Ther Nucleic Acids. 29:643. PubMed
  288. Pereira M, et al. 2022. Cell Rep. 40:111225. PubMed
  289. Kim S, et al. 2022. Redox Biol. 54:102347. PubMed
  290. Matsumoto K, et al. 2017. PLoS One. 12(6):e0175406. PubMed
  291. Sauma D, et al. 2017. Immunol Res. 10.1007/s12026-017-8936-9. PubMed
  292. Gil-Pulido J, et al. 2017. PLoS One.. 10.1371/journal.pone.0181947. PubMed
  293. Yu L, et al. 2019. Elife. 8:e46677. PubMed
  294. Chen JC, et al. 2018. Front Immunol. 0.665277778. PubMed
  295. Peng L, et al. 2019. Mol Med Rep. 19:2876. PubMed
  296. Shin J, et al. 2018. Diabetes. 67:1068. PubMed
  297. Dutow P, et al. 2014. J Infect Dis. 209:1269. PubMed
  298. Huang H, et al. 2014. Antimicrob Agents Chemother. 58:1538. PubMed
  299. Lehmann GL, et al. 2020. J Exp Med. 217:00:00. PubMed
  300. Zhao L, et al. 2020. EBioMedicine. 60:103020. PubMed
  301. Riedelberger M, et al. 2020. Bio Protoc. e3552:10. PubMed
  302. Yan W, et al. 2021. Front Med (Lausanne). 8:752223. PubMed
  303. Sun C, et al. 2022. J Adv Res. 35:71. PubMed
  304. Lüönd F, et al. 2022. STAR Protoc. 3:101438. PubMed
  305. Germundson DL, et al. 2022. Front Allergy. 3:870513. PubMed
  306. Sundararaj K, et al. 2021. Immunology. 162:418. PubMed
  307. Jeong M, et al. 2022. JCI Insight. 7:. PubMed
  308. Moon S, et al. 2021. J Exp Med. 218:. PubMed
  309. Hu CF, et al. 2021. Front Immunol. 12:638381. PubMed
  310. Zheng J, et al. 2020. Nature. . PubMed
  311. Unudurthi SD, et al. 2020. Life Sci. 117440:247. PubMed
  312. Yamakawa D, et al. 2021. Cell Reports. 34(10):108817. PubMed
  313. Troutman TD, et al. 2021. STAR Protocols. 2(1):100363. PubMed
  314. Li X, et al. 2020. Nat Commun. 4.877777778. PubMed
  315. Siggs O, et al. 2016. Proc Natl Acad Sci U S A. 113: 3706 - 3715. PubMed
  316. Seki T, et al. 2015. J Biochem. 158: 485 - 495. PubMed
  317. Cekanaviciute E, et al. 2014. J Immunol. 193:139. PubMed
  318. Hegde V, et al. 2013. J Biol Chem. 288:36810. PubMed
  319. Kyburz A, et al. 2019. J Allergy Clin Immunol. 143:1496. PubMed
  320. Liu C et al. 2019. Immunity. 51(2):381-397 . PubMed
  321. Chu A, et al. 2021. J Reprod Immunol. 145:103309. PubMed
  322. Frost JN, et al. 2021. Med (N Y). 2:164. PubMed
  323. Enomoto D, et al. 2017. PLoS One.. 10.1371/journal.pone.0183584. PubMed
  324. Wang Z et al. 2018. Immunity. 49(1):80-92 . PubMed
  325. Zeng J, et al. 2018. J Immunol. 200:3407. PubMed
  326. Hsu BE et al. 2019. Cell Rep. 27(13):3902-3915 . PubMed
  327. Di Mitri D, et al. 2019. Cell Rep. 28:2156. PubMed
  328. Rebecca S Dickinson et al. 2017. Wellcome open research. 0.155555556 . PubMed
  329. Li B, et al. 2019. Oncol Rep. 41:608. PubMed
  330. Kretschmer L, et al. 2020. Nat Commun. 0.536805556. PubMed
  331. Fujimoto N, et al. 2020. PLoS Biol. 18:e3000704. PubMed
  332. Akk A, et al. 2019. Mol Immunol. 114:629. PubMed
  333. Kmieciak M, et al. 2011. J Vis Exp. 47: 2381. PubMed
  334. Huang BR, et al. 2020. Int J Mol Sci. 21:00. PubMed
  335. Lindborg JA, et al. 2021. Cell Reports. 34(9):108777. PubMed
  336. Ahn D, et al. 2021. Cell Reports. 35(9):109196. PubMed
  337. Tuong ZK, et al. 2021. iScience. 24:103326. PubMed
  338. Yu H, et al. 2021. Front Oncol. 11:736882. PubMed
  339. Shiozawa S, et al. 2022. iScience. 25:103537. PubMed
  340. Blanchard L, et al. 2022. STAR Protoc. 3:101444. PubMed
  341. Babagana M, et al. 2021. Aging (Albany NY). 13:19207. PubMed
  342. Han Y, et al. 2022. Int J Biol Sci. 18:5653. PubMed
  343. Saveljeva S, et al. 2022. Cell Metab. 34:106. PubMed
  344. Zhang YN, et al. 2021. Sci Adv. 7:eabj3107. PubMed
  345. Shibuya M, et al. 2021. iScience. 24:103131. PubMed
  346. Ben-Yehuda H, et al. 2021. Mol Neurodegener. 16:39. PubMed
  347. Mohammadi MR, et al. 2021. Commun Biol. 4:685. PubMed
  348. Vilcaes AA, et al. 2020. Int J Mol Sci. :21. PubMed
  349. Zhao L, et al. 2021. STAR Protocols. 2:100732. PubMed
  350. Desai P, et al. 2021. Cell. 184(5):1214-1231.e16. PubMed
  351. Lehmann ML, et al. 2020. Brain Behav Immun. 88:735. PubMed
  352. Xu G, et al. 2020. Cell Rep. 32:108170. PubMed
  353. Takei S, et al. 2014. Vaccine. 32:3066. PubMed
  354. Constantinides M, et al. 2011. J Immunol. 187:309. PubMed
  355. Lenert R 2005. J Immunol. 174:2429. PubMed
  356. Ramachandran P, et al. 2019. Nature. 575:512. PubMed
  357. Spranger S, et al. 2017. Cancer Cell. 31:711. PubMed
  358. Burgener SS, et al. 2019. Cell Rep. 27:3646. PubMed
  359. Sanchez M et al. 2017. Cell host & microbe. 22(4):543-551 . PubMed
  360. Shibamoto M, et al. 2019. Int Heart J. 60:944. PubMed
  361. Matsuda T, et al. 2022. iScience. 25:105324. PubMed
  362. Takahashi F, et al. 2022. iScience. 25:104278. PubMed
  363. Okuma A, et al. 2017. Nat Commun.. 10.1038/s41467-017-02281-x. PubMed
  364. Kang YH, et al. 2019. Nat Commun. 10:912. PubMed
  365. Tellechea M, et al. 2018. J Immunol. 200:305. PubMed
  366. Oliveira AC et al. 2017. eLife. 6 pii: e30883. PubMed
  367. Lyle C, et al. 2019. Sci Rep. 9:20257. PubMed
  368. Roussey JA, et al. 2017. J Immunol. 199:3535. PubMed
  369. Mandour MF, et al. 2020. Infect Agent Cancer. 15:30. PubMed
  370. Kolter J, et al. 2020. Immunity. 50(6):1482-1497. PubMed
  371. Nakav S, et al. 2009. Nephrol Dial Transplant. 25:931. PubMed
  372. Miranda-Hernandez S, et al. 2011. J Immunol. 187:791. PubMed
  373. Miura N, et al. 2017. Mol Ther. 10.1016/j.ymthe.2017.01.020. PubMed
  374. Liu P, et al. 2020. Mol Cell. 748:77. PubMed
  375. Zhang Y, et al. 2020. Cancer Res. 80:3345. PubMed
  376. Bi J, et al. 2022. Front Microbiol. 13:927122. PubMed
  377. Zhang MH, et al. 2020. Front Immunol. 11:560. PubMed
  378. Tomimatsu M, et al. 2022. Sci Rep. 12:16656. PubMed
  379. López-Sanz C, et al. 2022. STAR Protoc. 3:101755. PubMed
  380. Ma L, et al. 2022. Sci Adv. 8:eabm3470. PubMed
  381. Leary N, et al. 2022. J Extracell Vesicles. 11:e12197. PubMed
  382. Lee SA, et al. 2021. Cells. 10:. PubMed
  383. Chida K, et al. 2021. Biol Pharm Bull. 44:1551. PubMed
  384. Saxena M, et al. 2020. Cancer Res. 80:3631. PubMed
  385. Chen C, et al. 2020. Cell Rep. 2136:30. PubMed
  386. Ouchi T, et al. 2011. J Exp Med. 208:2607. PubMed
  387. Baumann D, et al. 2020. Nat Commun. 1.969444444. PubMed
  388. Forster R, et al. 2019. FASEB J. 33:11210. PubMed
  389. Heinen A, et al. 2019. Mol Ther. 27:46:00. PubMed
  390. Simmons S et al. 2019. Elife. 8 pii: e41239. PubMed
  391. William M, et al. 2018. J Immunol. 200:4102. PubMed
  392. Contreras NA, et al. 2019. PLoS Pathog. 15:e1007890. PubMed
  393. Miura Y et al. 2018. eLife. 7 pii: e36572. PubMed
  394. Zhao N, et al. 2018. J Clin Invest. 26:84. PubMed
  395. Alshetaiwi H, et al. 2020. Methods Mol Biol. 2236:177. PubMed
  396. Gaya M et al. 2018. Cell. 172(3):517-533 . PubMed
  397. Freeman SA et al. 2018. Cell. 172(1-2):305-317 . PubMed
  398. Glodde N et al. 2017. Immunity. 47(4):789-802 . PubMed
  399. Knox T, et al. 2019. Sci Rep. 9:6136. PubMed
  400. Rojo R, et al. 2019. Nat Commun. 10:3215. PubMed
  401. Schaftenaar FH, et al. 2019. Sci Rep. 9:17391. PubMed
  402. Deng M, et al. 2019. J Exp Med. 216:2838. PubMed
  403. Hong JP, et al. 2020. Cell Reports Medicine. 1(3):100035. PubMed
  404. Clements J, et al. 2006. J Immunol. 177:905. PubMed
  405. Kimura T, et al. 2014. Int Immunol. 26:697. PubMed
  406. Howe C, et al. 2016. Sci Rep. 6:28699. PubMed
  407. Blaszczak AM, et al. 2020. Immunometabolism. 2:00. PubMed
  408. Senatus L, et al. 2020. JCI Insight. 5:00. PubMed
  409. Matsumoto M, et al. 2021. Cell Host Microbe. 29(6):930-940.e4. PubMed
  410. Jin HS, et al. 2020. Cancer Immunol Res. 8:912. PubMed
  411. Sakurai Y, et al. 2021. Pharmaceutics. 13:. PubMed
  412. Banskota S, et al. 2021. Inflamm Bowel Dis. 27:914. PubMed
  413. Magaletta ME, et al. 2022. Nat Commun. 13:457. PubMed
  414. Tzeng TT, et al. 2022. NPJ Vaccines. 7:60. PubMed
  415. Dong H, et al. 2022. Nat Metab. 4:90. PubMed
  416. Chetty A, et al. 2021. Cell Host Microbe. 29:579. PubMed
  417. Qiu Z, et al. 2020. Cancer Genet. 245:6. PubMed
  418. Kohn M, et al. 2021. Front Immunol. 626627:12. PubMed
  419. Bonavita E, et al. 2020. Immunity. 1215:53. PubMed
  420. Luo X, et al. 2021. Neuron. 109:2691. PubMed
  421. McDonald MM, et al. 2021. Cell. 184(5):1330-1347.e13. PubMed
  422. Alam MM, et al. 2021. eneuro. 8(1):. PubMed
  423. Sitapara RA, et al. 2020. Mol Med. 26:63. PubMed
  424. Kim K, et al. 2015. Infect Immun . 83:1150. PubMed
  425. Penzo M, et al. 2010. J Immunol. 184:4497. PubMed
  426. Mehrpouya-Bahrami P, et al. 2019. J Biol Chem. 294:7669. PubMed
  427. Burton OT, et al. 2018. Clin Exp Allergy. 48:288. PubMed
  428. Blaszczak AM, et al. 2019. J Immunol. 202:2451. PubMed
  429. Sivaram N, et al. 2019. J Clin Invest. 130. PubMed
  430. Litwinoff EMS, et al. 2017. Obes Res Clin Pract. 12:174. PubMed
  431. Burl RB et al. 2018. Cell metabolism. 28(2):300-309 . PubMed
  432. Chryplewicz A, et al. 2022. Cancer Cell. 40:1111. PubMed
  433. Sheng D, et al. 2022. J Immunother Cancer. 10:. PubMed
  434. Li Z et al. 2018. Immunity. 49(4):640-653 . PubMed
  435. Lyons DO, et al. 2018. PLoS One. 13:e0207704. PubMed
  436. Li A, et al. 2018. Cancer Lett. 431:54. PubMed
  437. Steinmann S, et al. 2020. Sci Rep. 1.160416667. PubMed
  438. Li J, et al. 2012. Arthritis Rheum. 64:1098. PubMed
  439. Melero I, et al. 2015. Cancer Res. 75:497. PubMed
  440. Hegde V, et al. 2015. J Immunol. 194:5211. PubMed
  441. Katsuyama T, et al. 2021. Cell Reports. 36(1):109339. PubMed
  442. Goldberg EL, et al. 2021. Cell Metabolism. :. PubMed
  443. Phadke MS, et al. 2021. Cancer Immunol Res. 9:554. PubMed
  444. Sibler E, et al. 2021. Cells. 10:. PubMed
  445. Qin J, et al. 2020. Cancer Immunol Res. 8:1426. PubMed
  446. Guo Y, et al. 2021. Nat Immunol. 22:746. PubMed
  447. Zhang M, et al. 2022. iScience. 25:104490. PubMed
  448. Kastenschmidt JM, et al. 2021. Cell Rep. 35:108997. PubMed
  449. Sibler E, et al. 2022. Cancers (Basel). 14:. PubMed
  450. Effern M, et al. 2020. Immunity. 53(3):564-580.e9. PubMed
  451. Liu Q, et al. 2016. Cell Death Dis. 1.93125. PubMed
  452. Richardson M, et al. 2014. PLoS Negl Trop Dis. 8:2825. PubMed
  453. Huanga J, et al. 2013. J Immunol Methods. 387:254. PubMed
  454. Malinczak CA, et al. 2019. J Immunol. 10.4049/jimmunol.1900741. PubMed
  455. Wang X, et al. 2018. Front Immunol. 9:2615. PubMed
  456. Kabir AU, et al. 2018. JCI Insight. 3. PubMed
  457. Buschor S, et al. 2017. PLoS Pathogens. 13(6):e1006476. PubMed
RRID
AB_312800 (BioLegend Cat. No. 101301)
AB_312800 (BioLegend Cat. No. 101302)

Antigen Details

Structure
Ig superfamily, 40-60 kD
Distribution

B cells, monocyte/macrophages, NK cells, neutrophils, mast cells, dendritic cells

Function
Low affinity receptors for IgG
Ligand/Receptor
IgG
Cell Type
B cells, Dendritic cells, Macrophages, Mast cells, Monocytes, Neutrophils, NK cells
Biology Area
Immunology, Innate Immunity
Molecular Family
CD Molecules, Fc Receptors
Antigen References

1. Barclay AN, et al. 1997. The Leukocyte Antigen FactsBook Academic Press.
2. Unkeless JC. 1989. J. Clin. Invest. 83:355.
3. Qiu WQ, et al. 1990. Science 248:732.

Gene ID
14130 View all products for this Gene ID 14131 View all products for this Gene ID
UniProt
View information about CD16/32 on UniProt.org
Go To Top Version: 2    Revision Date: 01.23.2015

For Research Use Only. Not for diagnostic or therapeutic use.

 

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