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Purified anti-mouse CD45 Antibody

Pricing & Availability
Clone
S18009D (See other available formats)
Regulatory Status
RUO
Other Names
T200, Ly-5, LCA
Isotype
Rat IgG2a, κ
Ave. Rating
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Product Citations
publications
S18009D_PURE_CD45_Antibody_1_072220
C57BL/6 splenocytes were stained with purified CD45 (clone S18009D) (filled histogram) or purified rat IgG2a, κ isotype control (open histogram), followed by anti-rat IgG PE.
  • S18009D_PURE_CD45_Antibody_1_072220
    C57BL/6 splenocytes were stained with purified CD45 (clone S18009D) (filled histogram) or purified rat IgG2a, κ isotype control (open histogram), followed by anti-rat IgG PE.
  • S18009D_PURE_CD45_Antibody_2_072220
    C57BL/6 mouse frozen intestine section was fixed with 4% paraformaldehyde (PFA) for 10 minutes at room temperature and blocked with 5% FBS for 30 minutes at room temperature. Then the section was stained with 10 µg/mL of purified anti-mouse CD45 (clone S18009D) overnight at 4°C, followed by 2.5 µg/mL of Alexa Fluor® 647 anti-mouse IgG (clone Poly4054) (red) for two hours at room temperature. Nuclei were counterstained with DAPI (green). The image was captured by 10X objective.
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160302 500 µg 175€
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Description

CD45 is a 180-240 kD glycoprotein also known as the leukocyte common antigen (LCA), T200, or Ly-5. It is a member of the protein tyrosine phosphatase (PTP) family, expressed on all hematopoietic cells except mature erythrocytes and platelets. There are different isoforms of CD45 that arise from variable splicing of exons 4, 5, and 6, which encode A, B, and C determinants, respectively. CD45 plays a key role in TCR and BCR signal transduction. These isoforms are very specific to the activation and maturation state of the cell as well as cell type. The primary ligands for CD45 are galectin-1, CD2, CD3, CD4, TCR, CD22, and Thy-1.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Recombinant mouse CD45
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/mL
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

FC - Quality tested
IHC-F - Verified
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.125 µg per million cells in 100 µL volume. For immunohistochemistry on frozen tissue sections, a concentration range of 5.0 - 10 µg/mL is suggested. It is recommended that the reagent be titrated for optimal performance for each application.

Product Citations
  1. Zhu S, et al. 2022. Front Pharmacol. 13:996635. PubMed
RRID
AB_2876568 (BioLegend Cat. No. 160302)

Antigen Details

Structure
Protein tyrosine phosphatase (PTP) family, 180-240 kD
Distribution

All hematopoietic cells except mature erythrocytes and platelets

Function
Phosphatase, T and B cell activation
Interaction
Mesenchymal Stem Cells, B cells, Dendritic cells, Tregs
Ligand/Receptor
Galectin-1, CD2, CD3, CD4, TCR, CD22, Thy-1
Cell Type
B cells, Dendritic cells, Mesenchymal Stem Cells, Tregs
Biology Area
Cell Biology, Immunology, Innate Immunity
Molecular Family
CD Molecules
Antigen References
  1. Barclay A, et al. 1997. The Leukocyte Antigen FactsBook Academic Press.
  2. Trowbridge IS, et al. 1993. Annu Rev Immunol. 12:85-116.
  3. Kishihara K, et al. 1993. Cell. 74:143-56.
  4. Pulido R, et al. 1988. J Immunol. 140:3851-7.
Gene ID
19264 View all products for this Gene ID
UniProt
View information about CD45 on UniProt.org
Go To Top Version: 1    Revision Date: 06.24.2020

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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