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Purified anti-Parkin Antibody (Previously Covance catalog# SIG-39530)

Pricing & Availability
Clone
Prk 8 (See other available formats)
Regulatory Status
RUO
Other Names
PARK2, AR-JP, LPRS2, PDJ, PRKN, E3 ubiquitin-protein ligase parkin, Parkin 2, E3 ubiquitin ligase, parkinson disease protein 2, parkinson juvenile disease protein 2, Parkinson disease (autosomal recessive, juvenile) 2, parkin
Previously
Signet Catalog# 9530-02
Signet Catalog# 9530-05
Signet Catalog# 9530-10
Covance Catalog# SIG-39530
Isotype
Mouse IgG2b, κ
Ave. Rating
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Product Citations
publications
Prk8_Purified_Parkin_Antibody_1_031919
Western blot of purified anti-Parkin antibody (clone Prk 8). Lane 1: Molecular weight marker; Lane 2: 20 µg of human brain lysate; Lane 3: 20 µg of mouse brain lysate; Lane 4: 20 µg of rat brain lysate. The blot was incubated with 1 µg/mL of the primary antibody overnight at 4°C, followed by incubation with HRP labeled goat anti-mouse IgG (Cat. No. 405306). Enhanced chemiluminescence was used as the detection system.
  • Prk8_Purified_Parkin_Antibody_1_031919
    Western blot of purified anti-Parkin antibody (clone Prk 8). Lane 1: Molecular weight marker; Lane 2: 20 µg of human brain lysate; Lane 3: 20 µg of mouse brain lysate; Lane 4: 20 µg of rat brain lysate. The blot was incubated with 1 µg/mL of the primary antibody overnight at 4°C, followed by incubation with HRP labeled goat anti-mouse IgG (Cat. No. 405306). Enhanced chemiluminescence was used as the detection system.
  • Prk8_Purified_Parkin_Antibody_2_031919
    Western blot of purified anti-Parkin antibody (clone Prk 8). Lane 1: Molecular weight marker; Lane 2: 20 µg of mouse skeletal muscle lysate. The blot was incubated with 1 µg/mL of the primary antibody overnight at 4°C, followed by incubation with HRP labeled goat anti-mouse IgG (Cat. No. 405306). Enhanced chemiluminescence was used as the detection system.
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808503 1 mL 1214€
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808504 25 µL 85€
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808501 200 µL 312€
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808502 500 µL 687€
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Description

Parkin (also known as PARK2, AR-JP, PRKN) is a protein encoded by the PARK2 gene in humans. It is a component of the E3 ubiquitin ligase complex that mediates the targeting of proteins for degradation. Mutations in the PARK2 gene cause a familial form of Parkinson’s disease called autosomal recessive juvenile Parkinson’s disease (AR-JP).

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human, Mouse, Rat
Antibody Type
Monoclonal
Host Species
Mouse
Formulation
Phosphate-buffered solution (no preservatives or carrier proteins).
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/ml
Storage & Handling
This antibody should be handled aseptically as it is free of preservatives such as Sodium Azide. Store this antibody undiluted between 2°C and 8°C. Please note the storage condition for this antibody has been changed from -20°C to between 2°C and 8°C. You can also check the vial label or CoA to find the proper storage conditions.
Application

WB - Quality tested
ICC, IHC-P - Reported in the literature, not verified in house
Recommended Usage

Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 1.0 - 5.0 µg per ml. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

This antibody is reactive to the R2 domain of human and murine parkin.

Application References
  1. Tay SP, et al. 2018. J Biol Chem. 285: 29231-29238. (WB, ICC)
  2. Pawlyk AC, et al. 2003. J Biol Chem. 278(48):48120-8. (WB, IHC-P)
RRID
AB_2564743 (BioLegend Cat. No. 808503)
AB_2564743 (BioLegend Cat. No. 808504)
AB_2564743 (BioLegend Cat. No. 808501)
AB_2564743 (BioLegend Cat. No. 808502)

Antigen Details

Biology Area
Cell Biology, Mitochondrial Function, Neurodegeneration, Neuroinflammation, Neuroscience, Neuroscience Cell Markers, Protein Trafficking and Clearance
Molecular Family
Mitochondrial Markers
Gene ID
5071 View all products for this Gene ID
UniProt
View information about Parkin on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 4    Revision Date: 07.09.2024

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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