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Purified anti-PLCγ1 Antibody

Pricing & Availability
Clone
W23013A (See other available formats)
Regulatory Status
RUO
Other Names
PLCG1, 1-phosphatidylinositol 4, 5-bisphosphate phosphodiesterase gamma-1, phospholipase C, gamma 1 (PLCG1), phospholipase C-II (PLC-II)
Isotype
Rat IgG2a, κ
Ave. Rating
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Product Citations
publications
a. W23013A_PURE_PLCg1_WB_052824
Whole cell extracts (15 µg total protein per lane) from indicated cell lines and tissues were resolved by 4-12% Bis-Tris gel electrophoresis, transferred to a PVDF membrane, and probed with Purified anti-PLCγ1 (clone W23013A) overnight at 4°C. Proteins were visualized by chemiluminescence detection using HRP Goat anti-rat IgG (Cat. No. 405405). Direct-Blot™ HRP anti-GAPDH (Cat. No. 607903) was used as a loading control. Western-Ready™ ECL Substrate Premium Kit (Cat. No. 426319) was used as a detection agent. Lane M: Molecular weight marker
  • a. W23013A_PURE_PLCg1_WB_052824
    Whole cell extracts (15 µg total protein per lane) from indicated cell lines and tissues were resolved by 4-12% Bis-Tris gel electrophoresis, transferred to a PVDF membrane, and probed with Purified anti-PLCγ1 (clone W23013A) overnight at 4°C. Proteins were visualized by chemiluminescence detection using HRP Goat anti-rat IgG (Cat. No. 405405). Direct-Blot™ HRP anti-GAPDH (Cat. No. 607903) was used as a loading control. Western-Ready™ ECL Substrate Premium Kit (Cat. No. 426319) was used as a detection agent. Lane M: Molecular weight marker
  • b. W23013A_PURE_PLCg1_IHC-P_052824
    IHC staining of Purified anti-PLCγ1 (clone W23013A) on formalin-fixed paraffin-embedded human tonsil tissue. Following antigen retrieval using Sodium Citrate H.I.E.R., 1X (Cat. No. 928502), the tissue was incubated without (panel A) and with (panel B) Purified anti-PLCγ1 (clone W23013A) followed by incubation with Alexa Fluor® 647 Goat anti-rat IgG (Cat. No. 405416) for 1 hour at room temperature. Nuclei were counterstained with DAPI (Cat. No. 422801). Images were captured with a 40X objective and merged. Scale bar: 50 µm
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638051 25 µg 140€
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638052 100 µg 344€
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Description

Phospholipase C gamma 1 (PLCγ1) is a signaling enzyme involved in intracellular signal transduction. In response to activation of receptor-type tyrosine kinases, phospholipase C (PLC) enzymes cleave the phospholipid phosphatidylinositol 4,5-bisphosphate (PIP2) into second messengers, namely inositol 1,4,5-triposphate (IP3) and diacylglycerol (DAG). IP3 causes the release of calcium from the endoplasmic reticulum to begin an intracellular signaling cascade, while DAG activates Protein Kinase C.

There are four families of PLCs. The PLCγ family, consisting of PLCγ1 and PLCγ2 in humans, uniquely contains SRC domains SH2 and SH3. Receptor tyrosine kinases interact with these SRC domains to enzymatically activate PLCγ1 via phosphorylation. PLCγ1 is essential for cell proliferation, differentiation, and motility. Overexpression or activation of PLCγ1 has been implicated in breast and prostate cancers as well as cancer cell invasion.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Recombinant fragment of the C-terminus of human PLCγ1
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/mL
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

WB - Quality tested 
IHC-P - Verified
Recommended Usage

Each lot of this antibody is quality control tested by western blotting. For western blotting, the suggested use of this reagent is 0.25 - 1.0 µg/mL. For immunohistochemistry on formalin-fixed paraffin-embedded tissue sections, a concentration range of 5.0 - 10.0 µg/mL is suggested. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

This antibody (clone W23013A) does not cross-react with mouse PLCγ1 in Western Blot.

This antibody (clone W23013A) is not suitable for either immunocytochemistry (ICC) or intracellular flow cytometry (ICFC) due to dim signal.

For immunohistochemistry (IHC), we recommend antigen retrieval with Sodium Citrate (Cat. No. 928502). Antigen retrieval with Tris-EDTA (10 mM Tris, 1 mM EDTA, 0.05% Tween-20, pH 9.0) is not recommended due to non-specific staining.

RRID
AB_3106246 (BioLegend Cat. No. 638051)
AB_3106246 (BioLegend Cat. No. 638052)

Antigen Details

Structure
PLCγ1 is a 1290 amino acid protein with a predicted molecular weight of 148.5 kD.
Distribution

Cytoplasmic

Function
Enzyme
Interaction
GRB2, and various Receptor tyrosine kinases (RTKs) such as PDGFRA, PDGFRB, EGFR, RET, FLT1, TRKA, BLNK, etc.
Cell Type
T cells
Biology Area
Cell Proliferation and Viability, Signal Transduction
Molecular Family
Protein Kinases/Phosphatase
Antigen References
  1. Ji QS, et al. 1997. Proc Natl Acad Sci. 94:2999-3003. 
  2. Jang HJ, et al. 2018. Adv Biol Regul. 67:179-189. 
  3. Yang YR, et al. 2016. Adv Biol Regul. 61:80-5. 
Gene ID
5335 View all products for this Gene ID
UniProt
View information about PLCgamma1 on UniProt.org
Go To Top Version: 1    Revision Date: 05.29.2024

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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