- Clone
- 2E9C01 (See other available formats)
- Regulatory Status
- RUO
- Other Names
- POU domain class 2-associating factor 1, B-cell-specific coactivator OBF-1 (OBF1), BOB-1, OCA-B, OCT-binding factor 1
- Isotype
- Mouse IgG1, κ
- Ave. Rating
- Submit a Review
- Product Citations
- publications
Cat # | Size | Price | Quantity Check Availability | Save | ||
---|---|---|---|---|---|---|
682502 | 100 µg | 278€ |
POU2AF1, also known as OBF1, is a B lymphocyte specific transcriptional coactivator. POU2AF1 plays important regulatory roles in the function and maturation of B cells. It stimulates transcription of the immunoglobulin genes by interaction with Oct-1 or Oct-2. The POU2AF1 gene encodes a single mRNA that produces two isoforms, p34 and p35, by post-translational processing. Both isoforms are expressed at similar levels and are able to stimulate octamer-dependent transcription. The cytoplasmic p35 isoform of POU2AF1 regulates pre-B cell proliferation and BCR signaling through interacting with the tyrosine kinase Syk. POU2AF1 deficient mice have shown reduced numbers of circulating B cells in the bone marrow and failed to form germinal centers in the spleen.
Product DetailsProduct Details
- Verified Reactivity
- Human, Mouse
- Antibody Type
- Monoclonal
- Host Species
- Mouse
- Immunogen
- Full length human POU2AF1 recombinant protein expressed in E. coli.
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
- Preparation
- The antibody was purified by affinity chromatography.
- Concentration
- 0.5 mg/ml
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C.
- Application
-
WB - Quality tested
- Recommended Usage
-
Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 0.1 - 10.0 µg per ml. It is recommended that the reagent be titrated for optimal performance for each application.
- Application Notes
-
The POU2AF1 gene encodes a single mRNA that produces two isoforms, p34 and p35. This antibody recognizes both.
- RRID
-
AB_2566625 (BioLegend Cat. No. 682502)
Antigen Details
- Structure
- 256 amino acids with a predicted molecular weight of 27.4 kD.
- Distribution
-
Nucleus.
- Function
- POU2AF1 is a B-cell specific transcription factor that participates in the regulation of T cell dependent humoral immune response and formation of the germinal center in the spleen.
- Interaction
- POU2AF1 interacts with OCT1, OCT2, SIAH1, SIAH2, and SYK.
- Cell Type
- B cells
- Biology Area
- Cell Biology, Immunology, Transcription Factors
- Molecular Family
- Nuclear Markers
- Antigen References
-
1. Corcoran L, et al. 2014. Front Immunol. 5:108.
2. Bordon A, et al. 2008. PLoS One 3:e4007.
3. Zhao C, et al. 2008. Oncogene. 27:63.
4. Lins K, et al. 2003. EMBO. J. 22:2188.
5. Nielsen PJ, et al. 1996. Eur. J. Immunol. 26:3214.
6. Francesconi SC, et al. 1991. Proc. Natl. Acad. Sci. USA 88:4089. - Gene ID
- 5450 View all products for this Gene ID
- UniProt
- View information about POU2AF1 on UniProt.org
Related Pages & Pathways
Pages
Other Formats
View All POU2AF1 (OBF1) Reagents Request Custom ConjugationDescription | Clone | Applications |
---|---|---|
Purified anti-POU2AF1 (OBF1) | 2E9C01 | WB |
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Purified anti-POU2AF1 (OBF1)
Total lysates (15 µg protein) from Daudi (Human, Positive co...
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