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- Clone
- 2E2.D11 (See other available formats)
- Regulatory Status
- RUO
- Other Names
- Serine/threonine-protein phosphatase 2B catalytic subunit alpha isoform, calcineurin A alpha, CAM-PRP catalytic subunit, calmodulin-dependent calcineurin A subunit alpha isoform, protein phosphatase 2B, protein phosphatase 3
- Previously
-
Covance Catalog# MMS-5123
- Isotype
- Mouse IgG1, κ
- Ave. Rating
- Submit a Review
- Product Citations
- publications
-
Western blot of purified anti-PPP3CA (CnA1) antibody (clone 2E2.D11). Lane 1: Molecular weight marker; Lane 2: 20 µg of human brain lysate; Lane 3: 20 µg of mouse brain lysate; Lane 4: 20 µg of rat brain lysate. The blot was incubated with 0.5 µg/mL of the primary antibody overnight at 4°C, followed by incubation with HRP goat anti-mouse IgG antibody (Cat. No. 405306). Enhanced chemiluminescence was used as the detection system. -
IHC staining of purified anti-PPP3CA (CnA1) antibody (clone 2E2.D11) on formalin-fixed paraffin-embedded mouse brain tissue. Following antigen retrieval using Sodium Citrate H.I.E.R., the tissue was incubated with 1 µg/mL of the primary antibody for 60 minutes at room temperature. BioLegend's Ultra-Streptavidin (USA) HRP Detection Kit (Multi-Species, DAB, Cat. No. 929901) was used for detection followed by hematoxylin counterstaining, according to the protocol provided. The image was captured with a 40X objective. Scale bar: 50 µm -
IHC staining of purified anti-PPP3CA (CnA1) antibody (clone 2E2.D11) on formalin-fixed paraffin-embedded rat brain tissue. Following antigen retrieval using Sodium Citrate H.I.E.R., the tissue was incubated with 1 µg/mL of the primary antibody for 60 minutes at room temperature. BioLegend's Ultra-Streptavidin (USA) HRP Detection Kit (Multi-Species, DAB, Cat. No. 929901) was used for detection followed by hematoxylin counterstaining, according to the protocol provided. The image was captured with a 40X objective. Scale bar: 50 µm
| Cat # | Size | Price | Quantity Check Availability | Save | ||
|---|---|---|---|---|---|---|
| 817601 | 100 µL | 220€ | ||||
PPP3CA (Protein Phosphatase 3, Catalytic Subunit, Alpha Isozyme) is also known by a handful of other names, including CALNA, PPP2B, Calcineurin A Alpha, and CNA1. It is one of the subunits of the heterodimer that forms calcineurin. Calcineurin functions as a calcium-dependent, calmodulin-stimulated protein phosphatase that activates T cells via dephosphorylation of the transcription factor NFATc.
Product DetailsProduct Details
- Verified Reactivity
- Human, Mouse, Rat
- Antibody Type
- Monoclonal
- Host Species
- Mouse
- Immunogen
- This monoclonal antibody was raised against a peptide sequence conjugated to KLH corresponding to amino acids 478-492 of human CnA1.
- Formulation
- Phosphate-buffered solution.
- Preparation
- The antibody was purified by affinity chromatography.
- Concentration
- 1 mg/mL
- Storage & Handling
- This antibody should be handled aseptically as it is free of preservatives such as Sodium Azide. Store this antibody undiluted between 2°C and 8°C. Please note the storage condition for this antibody has been changed from -20°C to between 2°C and 8°C. You can also check the vial label or CoA to find the proper storage conditions.
- Application
-
WB - Quality tested
IHC-P - Verified - Recommended Usage
-
Each lot of this antibody is quality control tested by western blotting. For western blotting, the suggested use of this reagent is 0.5 - 1 µg/mL. For immunohistochemistry, a concentration range of 1 - 5 µg/mL is suggested. It is recommended that the reagent be titrated for optimal performance for each application.
- RRID
-
AB_2564812 (BioLegend Cat. No. 817601)
Antigen Details
- Structure
- Expected MW: 60 kD
- Biology Area
- Cell Biology, Neuroscience, Synaptic Biology
- Molecular Family
- Postsynaptic proteins
- Gene ID
- 5530 View all products for this Gene ID
- UniProt
- View information about PPP3CA on UniProt.org
Other Formats
View All PPP3CA Reagents Request Custom Conjugation| Description | Clone | Applications |
|---|---|---|
| Purified anti-PPP3CA (CnA1) | 2E2.D11 | WB,IHC-P |
Compare Data Across All Formats
This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.
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