- Clone
- W22030A (See other available formats)
- Regulatory Status
- RUO
- Other Names
- AKT1S1, AKT1 Substrate 1, PRAS40, 40 KDa Proline-Rich AKT Substrate, AKT1 Substrate 1, Proline-Rich AKT1 Substrate 1, MGC2865
- Isotype
- Rat IgG2a, κ
- Ave. Rating
- Submit a Review
- Product Citations
- publications
Cat # | Size | Price | Quantity Check Availability | Save | ||
---|---|---|---|---|---|---|
667651 | 25 µg | 132€ | ||||
667652 | 100 µg | 332€ |
Numerous growth factors and hormones have the capability to initiate the phosphoinositide 3-kinase signaling pathway, leading to the activation of downstream effector proteins such as the serine/threonine kinase AKT. A 40 kDa proline-rich protein called PRAS40 (also known as AKT1S1), which interacts with 14-3-3 proteins, is one known AKT substrate. In order to transduce AKT signals to the mTOR complex, PRAS40/AKT1S1 interacts with mTOR which regulates cell growth and cell cycle and acts as a negative regulator of autophagy. Inhibition of mTOR signaling promotes PRAS40/AKT1S1 binding to mTOR, which reduces mTOR activity. In PC12 cells, treatment with insulin-like growth factor-1 (IGF1) can cause phosphorylation of PRAS40/AKT1S1 by the PI3K/AKT signaling pathway. In insulin-deficient cells, PRAS40/AKT1S1 interacts with raptor in mTOR complex 1 (mTORC1) and prevents the cell cycle protein Rheb from activating the mTORC1 pathway. The inhibition of mTORC1 by PRAS40/AKT1S1 is relieved when AKT phosphorylates PRAS40/AKT1S1 at Thr246. Additionally, PRAS40/AKT1S1 is phosphorylated on Ser183, Ser212, and Ser221 by mTORC1, which releases it from mTORC1.
Product DetailsProduct Details
- Verified Reactivity
- Human, Mouse, Rat
- Antibody Type
- Monoclonal
- Host Species
- Rat
- Immunogen
- Recombinant human PRAS40/AKT1S1 protein
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
- Preparation
- The antibody was purified by affinity chromatography.
- Concentration
- 0.5 mg/mL
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C.
- Application
-
WB - Quality tested
ICC - Verified - Recommended Usage
-
Each lot of this antibody is quality control tested by western blotting. For western blotting, the suggested use of this reagent is 0.125 - 1.0 µg/mL. For immunocytochemistry, a concentration range of 1.0 - 5.0 μg/mL is recommended. It is recommended that the reagent be titrated for optimal performance for each application.
- Application Notes
-
For use of this antibody (clone W22030A) in immunocytochemistry (ICC), 4% PFA fixation followed by permeabilization with ice-cold methanol is recommended.
This antibody (clone W22030A) is not suitable for use in immunohistochemistry (IHC). - Additional Product Notes
-
This antibody has been tested in knockout/knockdown models for WB and ICC.
- RRID
-
AB_3662335 (BioLegend Cat. No. 667651)
AB_3662335 (BioLegend Cat. No. 667652)
Antigen Details
- Structure
- PRAS40/AKT1S1 is a 256 amino acid protein with a predicted molecular weight of 27 kD.
- Distribution
-
Widely expressed with highest levels of expression in the liver and heart
- Function
- Negative regulator of mTORC1 and plays a role in nerve growth factor-mediated neuroprotection
- Interaction
- mTORC1, MTOR, MLST8, RPTOR
- Cell Type
- Neurons
- Biology Area
- Cell Biology, Cell Proliferation and Viability, Neuroscience, Signal Transduction
- Molecular Family
- Adaptor Proteins
- Antigen References
-
- Cantley LC. 2002. Science. 296: 1655-7.
- Kovacina KS, et al. 2003. J Biol Chem. 278:10189-94.
- Vander Haar E, et al. 2007. Nat Cell Biol. 9:316-23.
- Sancak Y, et al. 2007. Mol Cell. 25:903-15.
- Oshiro N, et al. 2007. J Biol Chem. 282:20329-39.
- Wang L, et al. 2008. J Biol Chem. 283:15619-27.
- Wang H, et al. 2012. Neurosci Lett. 516:105-9.
- Gene ID
- 84335 View all products for this Gene ID
- UniProt
- View information about PRAS40 on UniProt.org
Other Formats
View All PRAS40 Reagents Request Custom ConjugationDescription | Clone | Applications |
---|---|---|
Purified anti-PRAS40/AKT1S1 | W22030A | WB,ICC |
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Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
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