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Purified anti-REEP1 Antibody

Pricing & Availability
Clone
N345/51 (See other available formats)
Regulatory Status
RUO
Other Names
Receptor Accessory Protein 1, Spastic Paraplegia 31 Protein, C2orf2, SPG31, Receptor Expression-Enhancing Protein 1, Chromosome 2 Open Reading Frame 23, HMN5B, Yip2a
Isotype
Mouse IgG2b, κ
Ave. Rating
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Product Citations
publications
N345-51_Purified_REEP1_Antibody_1_112018
Western blot of purified anti-REEP1 antibody (clone N345/51). Lane 1: Molecular weight marker; Lane 2: 40 µg of human brain lysate; Lane 3: 20 µg of mouse brain lysate; Lane 4: 20 µg of rat brain lysate. The blot was incubated with 1 µg /mL of the primary antibody overnight at 4°C, followed by incubation with HRP-labeled goat anti-mouse IgG (Cat. No. 405306). Enhanced chemiluminescence was used as the detection system.
  • N345-51_Purified_REEP1_Antibody_1_112018
    Western blot of purified anti-REEP1 antibody (clone N345/51). Lane 1: Molecular weight marker; Lane 2: 40 µg of human brain lysate; Lane 3: 20 µg of mouse brain lysate; Lane 4: 20 µg of rat brain lysate. The blot was incubated with 1 µg /mL of the primary antibody overnight at 4°C, followed by incubation with HRP-labeled goat anti-mouse IgG (Cat. No. 405306). Enhanced chemiluminescence was used as the detection system.
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857901 25 µg 67€
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857902 100 µg 132€
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Description

Receptor expression enhancing protein 1 (REEP1) is a multi-pass mitochondrion membrane protein that is part of the DP1 family. REEP1 has been reported to interact and enhance the cell surface expression of odorant receptors.  Interestingly, REEP1 mutations have been linked to neurodegenerative disorders of upper and lower motor neurons, hereditary spastic paraplegia (HSP), and distal hereditary motor neuropathy type V (dHMN-V) (1).

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human, Mouse, Rat
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Mouse recombinant protein fragment corresponding to amino acids 111-201.
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

WB - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 1.0 - 5.0 µg per ml. It is recommended that the reagent be titrated for optimal performance for each application.

RRID
AB_2801217 (BioLegend Cat. No. 857901)
AB_2801217 (BioLegend Cat. No. 857902)

Antigen Details

Structure
REEP1 is a 201 amino acid protein with a molecular mass of ~22 kD.
Distribution

Tissue Distribution: Cerebellum, smooth muscle, stomach, colon, and testis.
Cellular distribution: Endoplasmic reticulum, mitochondrion, and plasma membrane.

Function
REEP1 is required for endoplasmic reticulum formation, shaping and remodeling by linking endoplasmic reticulum tubules to the cytoskeleton. REEP1 may also enhance the cell surface expression of odorant receptors.
Interaction
REEP1 interacts with SPAST, ALT1, microtubules, odorant receptors, and ZFYVE27.
Biology Area
Cell Biology, Mitochondrial Function, Neuroscience
Antigen References
  1. Carl M Hurt, et al. 2014. Brain Res. 2014 1545: 12–22
  2. Pengli Zheng, et al. 2018. Cell Res. 28(8): 833–854.
  3. Lavie J, et al. 2017. Hum Mol Genet. 15;26(4):674-685.
  4. Youngshin Lim, et al. 2015. Ann Neurol. 78(5): 679–696.
Gene ID
65055 View all products for this Gene ID
UniProt
View information about REEP1 on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 1    Revision Date: 11.21.2018

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
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