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Purified anti-RET Antibody

Pricing & Availability
Clone
W22020E (See other available formats)
Regulatory Status
RUO
Other Names
CDHF12, CDHR16, PTC, RET51, Proto-oncogene tyrosine-protein kinase receptor Ret, RET receptor Tyrosine Kinase, RET tyrosine kinase receptor, proto-oncogene C-Ret, , RET-ELE1, RET9, Cadherin Family member 12
Isotype
Rat IgG2b, κ
Ave. Rating
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Product Citations
publications
W22020E_PURE_RET_WB_031924
Whole cell extracts (15 µg total protein) from indicated cell lines were resolved by 4-12% Bis-Tris gel electrophoresis, transferred to a PVDF membrane, and probed with Purified anti-RET (clone W22020E) overnight at 4°C. Proteins were visualized by chemiluminescence detection using HRP Goat anti-rat IgG (Cat. No. 405405). Direct-Blot™ HRP anti-GAPDH (Cat. No. 607903) was used as a loading control. Western-Ready™ ECL Substrate Premium Kit (Cat. No. 426319) was used as a detection agent. Lane M: Molecular weight marker
  • W22020E_PURE_RET_WB_031924
    Whole cell extracts (15 µg total protein) from indicated cell lines were resolved by 4-12% Bis-Tris gel electrophoresis, transferred to a PVDF membrane, and probed with Purified anti-RET (clone W22020E) overnight at 4°C. Proteins were visualized by chemiluminescence detection using HRP Goat anti-rat IgG (Cat. No. 405405). Direct-Blot™ HRP anti-GAPDH (Cat. No. 607903) was used as a loading control. Western-Ready™ ECL Substrate Premium Kit (Cat. No. 426319) was used as a detection agent. Lane M: Molecular weight marker
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635751 25 µg 140€
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635752 100 µg 392€
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Description

The proto-oncogene RET encodes a transmembrane tyrosine-protein kinase receptor that is involved in several cellular mechanisms such as proliferation, migration, neuronal navigation, and cell differentiation. RET is important in the development of the nervous system, as well as in the formation of organs and tissues derived from the neural crest. The interaction with the ligand glia cell-line derived neurotrophic factor (GDNF), is vital for promoting cell survival. Conversely, in the absence of GDNF the receptor becomes active and induces apoptosis. Oncogenic activation of RET by either cytogenetic rearrangement or activating mutations, is associated to various diseases such as Hirschsprung disease, central hypoventilation syndrome, and multiple endocrine neoplasia type IIA and type IIB.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Recombinant fragment of RET protein
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/mL
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

WB - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by western blotting. For western blotting, the suggested use of this reagent is 0.5 - 1.0 µg/mL. It is recommended that the reagent be titrated for optimal performance for each application.

RRID
AB_3106121 (BioLegend Cat. No. 635751)
AB_3106121 (BioLegend Cat. No. 635752)

Antigen Details

Structure
RET gene encodes a 1114 amino acid protein of around 124 kD.
Distribution

Cellular and endosomal membranes

Function
Receptor tyrosine-protein kinase
Interaction
PLCG1 and GRB7, PTK2/FAK1, AIP, ARTN, DOK2, DOK4, DOK5, GFRAL, GFRA1, GDNF
Ligand/Receptor
GDNF
Biology Area
Cancer Biomarkers, Cell Biology, Cell Death
Antigen References
  1. Novello S, et al. 2023. Oncologist. 28:402-413.
  2. Subbiah V, et al. 2020. J Clin Oncol. 38:1209-1221.
  3. Regua AT, et al. 2022. Front Oncol. 12:932353.
  4. Addeo A, et al. 2023. Pharmacol Ther. 242:108344.
Gene ID
5979 View all products for this Gene ID
UniProt
View information about RET on UniProt.org
Go To Top Version: 1    Revision Date: 03.19.2024

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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