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Purified anti-RGS14 Antibody (Previously Covance catalog# MMS-5248)

Pricing & Availability
Clone
N133/21 (See other available formats)
Regulatory Status
RUO
Other Names
Regulator of G-protein signaling 14, RAP1/RAP2-interacting protein
Previously
Covance Catalog# MMS-5248
Isotype
Mouse IgG2a, κ
Ave. Rating
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Product Citations
publications
N133.21_Purified_RGS14_IHC_020315.jpg
IHC staining of anti-RGS14 antibody (clone N133/21) on formalin-fixed paraffin-embedded rat brain tissue. Following antigen retrieval using Sodium Citrate H.I.E.R, the tissue was incubated with the primary antibody at 10 µg/mL overnight at 4°C. BioLegend's Ultra Streptavidin (USA) HRP Detection Kit (Multi-Species) was used for detection followed by hematoxylin and bluing solution counterstaining, according to the protocol provided.
  • N133.21_Purified_RGS14_IHC_020315.jpg
    IHC staining of anti-RGS14 antibody (clone N133/21) on formalin-fixed paraffin-embedded rat brain tissue. Following antigen retrieval using Sodium Citrate H.I.E.R, the tissue was incubated with the primary antibody at 10 µg/mL overnight at 4°C. BioLegend's Ultra Streptavidin (USA) HRP Detection Kit (Multi-Species) was used for detection followed by hematoxylin and bluing solution counterstaining, according to the protocol provided.
  • N133slash21_PURE_RGS14_Antibody_IHC_2_100517
    IHC staining of anti-RGS14 antibody (clone N133/21) on formalin-fixed paraffin-embedded mouse brain tissue. Following antigen retrieval using Sodium Citrate H.I.E.R, the tissue was incubated with the primary antibody at 10 µg/mL overnight at 4°C. BioLegend's Ultra Streptavidin (USA) HRP Detection Kit (Multi-Species) was used for detection followed by hematoxylin and bluing solution counterstaining, according to the protocol provided.
  • N133slash21_PURE_RGS14_Antibody_WB_100517
    Western blot of anti-RGS14 antibody (clone N133/21). Lane 1: molecular weight marker; Lane 2: 20µg of mouse brain lysate; Lane 3: 20µg of rat brain lysate. Blots were incubated with 10 µg/mL of primary antibody overnight at 4°C, followed by incubation with horseradish peroxidase labeled goat anti-mouse secondary antibody. Enhanced chemiluminescence was used as the detection system.
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821801 100 µL 132€
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Description

Regulator of G protein Signaling 14 (RGS14) modulates G-protein signaling by binding to G alpha subunits bound to GTP, converting GDP to GTP, and allowing G alpha subunits to bind to G beta/gamma subunits, which are inactive as a G protein.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Mouse, Rat
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
This monoclonal antibody was raised against a fusion protein corresponding to amino acids 1-544 (full-length) of rat RGS14.
Formulation
Phosphate-buffered solution.
Preparation
The antibody was purified by affinity chromatography.
Concentration
1 mg/ml
Storage & Handling
This antibody should be handled aseptically as it is free of preservatives such as Sodium Azide. Store this antibody undiluted between 2°C and 8°C. Please note the storage condition for this antibody has been changed from -20°C to between 2°C and 8°C. You can also check the vial label or CoA to find the proper storage conditions.
Application

IHC-P - Quality tested
WB - Verified
Recommended Usage

Each lot of this antibody is quality control tested by immunohistochemical staining. 

The optimal working dilution should be determined for each specific assay condition. 
• WB: 5-10 μg/ml
• IHC: 5-10 μg/ml
Tissue: Formalin-fixed Paraffin-embedded tissues 
Pre-treatment: Citrate 
Primary incubation: 24 hours at 4°C

Application Notes

This antibody is effective in immunoblotting (WB) and immunohistochemistry (IHC).

*Expected Molecular Weight = 60 kD

WB positive control: Rat and mouse brain membrane lysates. This clone does not cross-react with human RGS14 in WB.

IHC positive control: Rat and mouse brain tissue.

Product Citations
  1. Okamoto K, et al. 2023. Sci Rep. 13:323. PubMed
RRID
AB_2564854 (BioLegend Cat. No. 821801)

Antigen Details

Structure
Expected MW: 60kD
Biology Area
Cell Biology, Neuroscience, Synaptic Biology
Molecular Family
Postsynaptic proteins
Gene ID
114705 View all products for this Gene ID
UniProt
View information about RGS14 on UniProt.org
Go To Top Version: 7    Revision Date: 07.09.2024

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

BioLegend, the BioLegend logo, and all other trademarks are property of BioLegend, Inc. or their respective owners, and all rights are reserved.

 

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Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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