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Purified anti-RhoA Antibody

Pricing & Availability
Clone
A18230D (See other available formats)
Regulatory Status
RUO
Other Names
Ras Homolog Family Member A, Transforming protein RhoA, Ras Homolog Gene Family, Member A, Aplysia Ras-Related Homolog 12, RHOH12, ARH12, ARHA, RHO12, Epididymis Secretory Sperm Binding Protein
Isotype
Mouse IgG1, κ
Ave. Rating
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Product Citations
publications
A. A18230D_PURE_RhoA_WB_031924
Whole cell extracts (15 µg total protein) from indicated cell lines were resolved by 4-12% Bis-Tris gel electrophoresis, transferred to a PVDF membrane, and probed with 0.5 µg/mL of Purified anti-RhoA (clone A18230D) overnight at 4°C. Proteins were visualized by chemiluminescence detection using HRP Goat anti-mouse IgG (Cat. No. 405306) at a 1:3000 dilution. Direct-Blot™ HRP anti-GAPDH (Cat. No. 607904) was used as a loading control at a 1:50000 dilution. Western-Ready™ ECL Substrate Premium Kit (Cat. No. 426319) was used as a detection agent. Lane M: Molecular weight marker
  • A. A18230D_PURE_RhoA_WB_031924
    Whole cell extracts (15 µg total protein) from indicated cell lines were resolved by 4-12% Bis-Tris gel electrophoresis, transferred to a PVDF membrane, and probed with 0.5 µg/mL of Purified anti-RhoA (clone A18230D) overnight at 4°C. Proteins were visualized by chemiluminescence detection using HRP Goat anti-mouse IgG (Cat. No. 405306) at a 1:3000 dilution. Direct-Blot™ HRP anti-GAPDH (Cat. No. 607904) was used as a loading control at a 1:50000 dilution. Western-Ready™ ECL Substrate Premium Kit (Cat. No. 426319) was used as a detection agent. Lane M: Molecular weight marker
  • B. A18230D_PURE_RhoA_ICC_031924
    HeLa cells were fixed with Fixation Buffer (Cat. No. 420801) and permeabilized with ice-cold methanol for 10 minutes, and blocked with 5% FBS for 1 hour at room temperature. Cells were then stained with 5 µg/mL of Purified anti-RhoA (clone A18230D), followed by incubation with 2.5 µg/mL of Alexa Fluor® 647 Goat anti-mouse IgG (Cat. No. 405322) (panel A) for 1 hour at room temperature. Nuclei were counterstained with DAPI (Cat. No. 422801) (panel B). Images were merged (panel C). The images were captured by a Revvity Operetta CLS™ High Content Analysis System with a 63X objective. Scale bar: 50 µm
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635851 25 µg 136€
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635852 100 µg 324€
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Description

RhoA, also known as Transforming protein RhoA, is a small GTPase that plays a crucial role in various cellular functions, such as cytoskeletal dynamics, cell migration, cell cycle regulation, and cellular development. It alternates between active and inactive states by binding to either GTP or GDP, respectively. It is primarily associated with the regulation of the cytoskeleton, particularly in the formation of actin stress fibers and actomyosin contractility. RhoA influences cell shape, polarity, and locomotion through its effects on actin organization. In human mesenchymal stem cells, RhoA activity switches the differentiation between adipogenesis or osteogenesis under actin-myosin-generated cytoskeletal tension via effector, Rho-kinases (ROCK). RhoA is essential for cleavage furrow formation and apical junctions in keratinocyte cell-cell adhesion. In the developing nervous system, p75NTR induces RhoA strengthening on actin assembly which limits growth cone mobility and neuronal elongation. RhoA is found overexpressed or hyperactivate in many types of cancers, including prostate cancer, gastric cancer, testis cancer, breast cancer, and chronic myeloid leukemia. A RhoA inhibitor targeting RhoA/Rho GTPase pathway aims to prevent breast cancer cell growth as a potential cancer drug.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human, Mouse
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Recombinant fragment of human RhoA
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/mL
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

WB - Quality tested
ICC - Verified
Recommended Usage

Each lot of this antibody is quality control tested by western blotting. For western blotting, the suggested use of this reagent is 0.5 - 1.0 µg/mL. For immunocytochemistry, a concentration range of 1.0 - 10.0 μg/mL is recommended. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

For use of this antibody (clone A18230D) in immunocytochemistry (ICC), it is recommended to fix cells with Fixation Buffer (Cat. No. 420801) followed by permeabilization with ice-cold methanol or fixation and permeabilization with ice-cold methanol only. 

This antibody is not recommended for immunohistochemistry on formalin-fixed paraffin-embedded tissues (IHC-P).

RRID
AB_3106120 (BioLegend Cat. No. 635851)
AB_3106120 (BioLegend Cat. No. 635852)

Antigen Details

Structure
Contains two switch regions, Switch I and Switch II, modified by its activation status
Distribution

Expressed widely in most tissues/Located primarily in cytoplasm, may detect at low expression levels in nucleus

Function
GTPase which regulates cytoskeleton organization/Regulated by guanine nucleotide exchange factors (GEFs)
Interaction
ROCK1, ROCK2, ARHGEF2, ARHGEF3, ARHGDIA, ARHGDIB, ARHGEF28
Cell Type
Neurons, Platelets
Biology Area
Apoptosis/Tumor Suppressors/Cell Death, Cardiovascular Biology, Cell Adhesion, Cell Motility/Cytoskeleton/Structure, Cell Structure, DNA Repair/Replication, Neuroscience, Synaptic Biology
Molecular Family
GTPases, Microtubules
Antigen References
  1. Yamashita T, et al. 1999. Neuron. 24:585-93.
  2. McBeath R, et al. 2004. Dev Cell. 6:483-95.
  3. Pertz O, et al. 2006. Nature. 440:1069-72.
  4. Dubash AD, et al. 2011. PLoS One. 6:e17380.
  5. Shimokawa H, et al. 2016. Circ Res. 118:352-66.
  6. Tsubaki M, et al. 2021. Biomedicines. 9:35.
Gene ID
387 View all products for this Gene ID
UniProt
View information about RhoA on UniProt.org
Go To Top Version: 1    Revision Date: 03.19.2024

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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