- Regulatory Status
- RUO
- Other Names
- Red blood cell lysing solution
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Cat # | Size | Price | Quantity Check Availability | Save | ||
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422401 | 50 mL | 113€ |
BioLegend RBC Lysis/Fixation Solution has been designed and optimized for one-step lysing of red blood cells (RBCs) and fixation of the remaining blood elements (such as leukocytes) following immunofluorescent staining with fluorochrome-conjugated antibodies. It can be used for either lyse/wash or lyse/no wash immunofluorescent staining procedures. This solution is supplied as a 10X concentrate. Dilute to 1X working solution with room temperature dI H20 prior to use.
Product DetailsProduct Details
- Formulation
- This RBC Lysis/Fixation Solution is supplied as a 10X solution and should be diluted to 1X in room temperature deionized water.
- Preparation
- The pH of the 1X solution should fall within the range of pH 7.1 - 7.4. Adjust the pH if necessary. Warm the 1X solution to room temperature prior to use.
- Storage & Handling
- Store RBC lysis buffer between 2°C and 8°C.
- Application
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FC - Quality tested
- Recommended Usage
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Whole Blood Lyse/Wash Procedure:
1. Prepare 1X RBC Lysis/Fixation solution by diluting 1 part of BioLegend RBC Lysis/Fixation solution with 9 parts of room temperature dI H20.
2. Mix 100 µl of whole blood with fluorochrome-conjugated antibody.
3. Incubate at room temperature, in the dark, for 20 minutes.
4. Add 2.0 mL 1X RBC Lysis/Fixation solution, gently mixing each tube immediately.
5. Incubate at room temperature, in the dark, for 15 minutes.
6. Centrifuge at 350Xg for five minutes. Discard supernatant.
7. Add 2 mL BioLegend Cell Staining Buffer (Cat No 420201).
8. Centrifuge at 350Xg for five minutes. Discard supernatant.
9. Resuspend cell pellet in 0.3-0.5 mL Cell Staining Buffer.
10. Analyze cells immediately by flow cytometry or store at 4°C, in the dark, for no longer than 12 hours.
Whole Blood Lyse/No Wash Procedure:
1. Prepare 1X RBC Lysis/Fixation solution by diluting 1 part of BioLegend RBC Lysis/Fixation solution with 9 parts room temperature dI H20.
2. Mix 50 µl of whole blood with fluorochrome-conjugated antibody.
3. Incubate at room temperature, in the dark, for 20 minutes.
4. Add 0.45 mL 1X RBC Lysis/Fixation solution, gently mixing each tube immediately.
5. Incubate at room temperature, in the dark, for 15 minutes.
6. Analyze samples by flow cytometry. - Application Notes
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The 1X Lysis/Fixation solution can be used within 1 month from the date of preparation. It can be stored at room temperature or between 2°C and 8°C.
- Application References
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- Horvath P, et al. 2012. J Infect Dis. PubMed
- Product Citations
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Antigen Details
- Gene ID
- NA
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