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Recombinant Human BMP-4 (Animal-Free)

Pricing & Availability
Regulatory Status
RUO
Other Names
Bone morphogenetic protein 4 (BMP4), Bone morphogenetic protein 2B (BMP-2B)
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Product Citations
publications
Cat # Size Price Quantity Check Availability Save
710002 10 µg 203€
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Description

BMP-4 is a member of the bone morphogenetic protein (BMP) family of cytokines, which belong to the transforming growth factor beta (TGF-β) superfamily. BMP-4 plays a crucial function in the chondrogenic and osteogenic differentiation of mesenchymal stem cells as well as in the maintenance of adult cartilage and bone. Binding of BMP-4 to the BMPR-I/BMPR-II receptor complex was reported to activate several signaling pathways, such as the Smad, the MAPK, the PI3K/Akt, and the Wnt pathways, which are involved in bone formation, cell proliferation, and cell differentiation. Genetic defects in BMP-4 have been associated with microphthalmia and orofacial cleft.

Product Details
Technical Data Sheet (pdf)

Product Details

Source
Human BMP-4, amino acids (Lys303-Arg408) (Accession# NP_001193.1), was expressed in E. coli.
Molecular Mass
The 106 amino acid recombinant protein has a predicted molecular mass of approximately 11.9 kD. Recombinant BMP-4 is disulphide bond-linked homodimer. The predicted N-terminal amino acid is Lys.
Purity
>98%, as determined by Coomassie stained SDS-PAGE gel and HPLC analysis.
Formulation
Lyophilized, carrier-free.
Endotoxin Level
Less than 0.1 ng per µg of protein.
Storage & Handling
Unopened vial can be stored at -20°C or -70°C. For maximum results, quick spin vial prior to opening. Reconstitute in 5 mM HCl, pH 3.0 to a concentration of 0.1-1.0 mg/ml. Do not vortex. It is recommended to further dilute in a buffer, such as 5% Trehalose, and store working aliquots at -20°C to -80°C. Avoid repeated freeze/thaw cycles.
Activity
ED50 = 5 - 10 ng/ml, corresponding to a specific activity of 1 - 2 x 105 units/mg, as measured by its ability to induce alkaline phosphatase production by ATDC-5 cells in a dose dependent manner.
Application

Bioassay
Application Notes

This product is reactive with human, mouse, and rat.

Product Citations
  1. Yuka Morioka, Jin-Min Nam, Takashi Ohashi 2017. PLoS One. 12(2):e017150. PubMed

Antigen Details

Structure
Growth factor
Distribution
BMP-4 is widely expressed.
Function
BMP-4 is a multifunctional growth factor and is involved in bone formation, tooth development, and fracture repair.
Interaction
BMP-4 forms homodiner and interacts with GREM2, HTRA1, HTRA3, SOSTDC1.
Ligand/Receptor
BMP-4 binds to the receptor BMPR-I and BMPR-II.
Bioactivity
Human BMP-4 is able to induce alkaline phosphatase production in ATDC-5 cells.
Cell Type
Neural Stem Cells, Mesenchymal Stem Cells, Hematopoietic stem and progenitors, Embryonic Stem Cells
Biology Area
Cell Biology, Neuroscience, Stem Cells, Synaptic Biology
Molecular Family
Growth Factors, Cytokines/Chemokines
Antigen References

1. Kishimoto KN, et al. 2013. Biomed. Res. Int. 2013:143742.
2. Kuroda K, et al. 2013. Skelet. Muscle. 3:5.
3. Wei X, et al. 2013. Cell. Prolif. 46:416.
4. Yacqub-Usman K, et al. 2012. Endocrinology. 153:3603.
5. Tan TW, et al. 2012. J. Cell Physiol. 227:2531.
6. Labeur M, et al. 2010. Mol. Cell. Endocrinol. 326:85.

Gene ID
652 View all products for this Gene ID
UniProt
View information about BMP-4 on UniProt.org

Related FAQs

Why choose BioLegend recombinant proteins?

     • Each lot of product is quality-tested for bioactivity as indicated on the data sheet.
     • Greater than 95% Purity or higher, tested on every lot of product.
     • 100% Satisfaction Guarantee for quality performance, stability, and consistency.
     • Ready-to-use liquid format saves time and reduces challenges associated with reconstitution.
     • Bulk and customization available. Contact us.
     • Learn more about our Recombinant Proteins.

How does the activity of your recombinant proteins compare to competitors?

We quality control each and every lot of recombinant protein. Not only do we check its bioactivity, but we also compare it against other commercially available recombinant proteins. We make sure each recombinant protein’s activity is at least as good as or better than the competition’s. In order to provide you with the best possible product, we ensure that our testing process is rigorous and thorough. If you’re curious and eager to make the switch to BioLegend recombinants, contact your sales representative today!

What is the specific activity or ED50 of my recombinant protein?

The specific activity range of the protein is indicated on the product datasheets. Because the exact activity values on a per unit basis can largely fluctuate depending on a number of factors, including the nature of the assay, cell density, age of cells/passage number, culture media used, and end user technique, the specific activity is best defined as a range and we guarantee the specific activity of all our lots will be within the range indicated on the datasheet. Please note this only applies to recombinants labeled for use in bioassays. ELISA standard recombinant proteins are not recommended for bioassay usage as they are not tested for these applications.

Have your recombinants been tested for stability?

Our testing shows that the recombinant proteins are able to withstand room temperature for a week without losing activity. In addition the recombinant proteins were also found to withstand four cycles of freeze and thaw without losing activity.

Does specific activity of a recombinant protein vary between lots?

Specific activity will vary for each lot and for the type of experiment that is done to validate it, but all passed lots will have activity within the established ED50 range for the product and we guarantee that our products will have lot-to-lot consistency. Please conduct an experiment-specific validation to find the optimal ED50 for your system.

How do you convert activity as an ED50 in ng/ml to a specific activity in Units/mg?

Use formula Specific activity (Units/mg) = 10^6/ ED50 (ng/mL)

Go To Top Version: 3    Revision Date: 05.06.2014

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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