Recombinant Mouse IL-1α (ELISA Std.)

Product Details

Source

Mouse IL-1α, amino acids Ser115-Ser270 (Accession # BC003727) was expressed in E. coli.

Molecular Mass

The 156 amino acid recombinant protein has a predicted molecular mass of 17,990 Da. The DTT-reduced and the non-reduced protein migrate at approximately 18kDa by SDS-PAGE. The N-terminal amino acid is Serine.

Purity

Purity is >98%, as determined by Coomassie stained SDS-PAGE.

Formulation

Lyophilized in sterile-filtered PBS, pH 7.2, containing 1% BSA, 0.09% sodium azide, and protease inhibitors.

Endotoxin Level

Endotoxin level is <0.1 EU/µg (<0.01ng/µg) protein as determined by the LAL method.

Concentration

Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)

Storage & Handling

Unopened vials can be stored between 2°C and 8°C until the expiration date. Prior to use, reconstitute the lyophilized powder with 0.2 mL of PBS containing a carrier protein (e.g., 1% BSA, protease free), pH7.4. Re-cap vial, vortex. Allow the reconstituted standard to sit at room temperature for 15 minutes, vortex again to mix completely. The reconstituted standard stock solution can be aliquoted into polypropylene vials and stored at -70°C for up to one month. Do not re-use diluted standards. Avoid repeated freeze/thaw cycles.

Application

ELISA

Recommended Usage

Each lot of this protein is quality control tested by ELISA assay. For use as an ELISA standard, a standard curve comprised of doubling dilutions from 250 pg/ml to 4 pg/ml is suggested. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

This IL-1a protein is useful as a standard for a mouse IL-1a sandwich ELISA, using unlabeled BL1a-72 antibody (catalog #512402) for capture and biotinylated BL1a-89 antibody (catalog #512504) for detection.

Antigen Details

Structure

Cytokine

Distribution

IL-1α is produced by monocytes, macrophages, keratinocytes, stimulated B lymphocytes, fibroblasts, endothelial cells, and epithelial cells. In addition, IL-1α is localized in various ovarian cell types such as the oocyte, granulosa, and theca cells, and ovarian cancer cells (1).

Function

The cytokine IL-1α is a key molecule both in the innate and in the adaptive immune response. IL-1α induces autonomous proliferation in vascular smooth muscle cells, T cell activation during Ag presentation, up-regulation of monocyte/macrophage-mediated tumor cytotoxicity, stimulation of osteoclast formation, and expression of Cxcl1 (kC) chemokine mRNA and protein in mouse granulosa cells (1,2).

Ligand/Receptor

Type I and II IL-1 receptors (IL-1RI and IL-1RII)

Biology Area

Cell Biology, Immunology, Innate Immunity, Neuroinflammation, Neuroscience, Stem Cells

Molecular Family

Cytokines/Chemokines

Antigen References

1. Soo DS and Roby KF, et al. Mol Endocri 20:2999-3013 2006.
2. Yatabe T, et al. Ann Rheum Dis published online 28 Jul 2008.
3. Ledford H et al, Nature 450:29-30 2007.
4. Boraschi B Tagliabue A et al, Vitam Horm 74:229-254 2006.

Gene ID

16175 View all products for this Gene ID

UniProt

View information about IL-1alpha on UniProt.org

Documentation

• Technical Data Sheet (pdf)
• Certificate of Analysis
• Material Safety Data Sheet

Reviews

Related Protocols

• Sandwich ELISA Protocol

Related Pages & Pathways

Related FAQs

Certificate of Analysis