Spark Blue™ 550 anti-rat CD90/mouse CD90.1 (Thy1.1) (Flexi-Fluor™) Antibody, CD90/CD90.1, OX-7

Pricing & Availability

Clone: OX-7 (See other available formats)
Regulatory Status: RUO
Other Names: Thy-1, Thy-1.1
Isotype: Mouse IgG1, κ
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Product Citations: publications

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285271

50 µg

269€

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Description

CD90, also known as Thy-1, is a 28-30 kD GPI-linked membrane glycoprotein. It is a member of the immunoglobulin superfamily and has been shown to interact with CD45 in signal transduction during lymphocyte proliferation and differentiation. CD90 is expressed on hematopoietic stem cells, neurons, thymocytes, peripheral T cells, fibroblasts, stromal cells.

Product Details

Technical Data Sheet (pdf)

Product Details

Verified Reactivity: Rat
Reported Reactivity: Rabbit, Guinea Pig
Antibody Type: Monoclonal
Host Species: Mouse
Immunogen: Rat thymocyte Thy-1 antigen
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
Preparation: The antibody was purified by affinity chromatography and conjugated with Spark Blue™ 550 under optimal conditions.
Concentration: 0.2 mg/mL
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: FC
Recommended Usage: Flexi-Fluors™ are provided at a standard 0.2 mg/mL concentration. We recommend titrating this reagent to determine the optimal concentration for each application. For many flow cytometry applications, conjugated antibodies perform well at concentrations ranging from 0.03 to 1.0 µg per million cells in 100 µL. We recommend testing a range of concentrations starting from 10 µg/mL.

For example, make five 1:1 serial dilutions of the 0.2 mg/mL antibody. Add 5 µL of each dilution (including the undiluted antibody) to 100 µL of cells (at 10⁷ cells/mL) to test six concentrations -- 1.0, 0.5, 0.25, 0.125, 0.06, and 0.03 µg per million cells in 100 µL volume. Compare staining patterns or create a titration curve using the MFI or staining index to determine the optimal concentration.
Excitation Laser: Blue Laser (488 nm)
Application Notes: The OX-7 antibody reacts with rat CD90 and mouse CD90.1 (Thy-1.1) (which is expressed by mouse strains of AKR/J, PL, and FVB/N), but not mouse CD90.2.

Additional reported applications (for the relevant formats) include: immunohistochemical⁷ and immunofluorescent⁸ staining of acetone-fixed frozen sections and zinc-fixed paraffin-embedded sections, immunoprecipitation¹, Western blotting¹, in vitro activation of leukocytes², induction of endothelial cell permeability³, induction of apoptosis in glomerular mesangial cells, and induction of glomerulonephritis in vivo⁴.
Additional Product Notes: For more information about Flexi-Fluors™, visit our Flexi-Fluor™ page and review FAQs associated with this product line.

Antigen Details

Structure: Ig superfamily, GPI-anchored membrane glycoprotein, 28-30 kD
Distribution: Hematopoietic stem cells, thymocytes, a subset of peripheral blood T cells, early myeloid and erythroid progenitors, immature B cells, neurons, activated endothelium, mast cells, dendritic cells, glomeruli
Function: Adhesion, signal transduction, lymphocyte co-stimulation, proliferation and differentiation of hematopoietic stem cells
Ligand/Receptor: Interacts with CD45
Cell Type: B cells, Dendritic cells, Endothelial cells, Hematopoietic stem and progenitors, Mast cells, Neurons, T cells, Thymocytes
Biology Area: Immunology
Molecular Family: CD Molecules
Antigen References: 1. Campbell DG, et al. 1981. Biochem. J. 195:15.
2. Hosseinzadeh H, et al. 1993. J. Immunol. 150:1670.
Gene ID: 21838 View all products for this Gene ID 24832 View all products for this Gene ID
UniProt: View information about CD90/CD90.1 on UniProt.org

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Related Protocols

Cell Surface Flow Cytometry Staining Protocol

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Description	Clone	Applications

Related Pages & Pathways

Related FAQs

What are Flexi-Fluors?

Flexi-Fluors are rapidly made-to-order conjugated antibodies. The technology, manufacturing processes, and specifications used to create Flexi-Fluors are the same as our regular catalog products. However, the optimal concentration and performance of each Flexi-Fluor must be determined by the customer.

How quickly will I receive my order?

We aim to ship Flexi-Fluors within 2-3 weeks of receipt of your order. However, depending on your location, shipping times may vary.

How are Flexi-Fluors different from regular catalog products?

Flexi-Fluors are made on demand, specifically for you. Flexi-Fluors are manufactured using the same high-quality standards, and specifications as other catalog products. For faster delivery, Flexi-Fluors are not tested by flow cytometry to determine optimal concentrations or evaluate performance. This testing needs to be performed by the customer.

How do I determine the optimal concentration for using my Flexi-Fluor? How should I titrate my antibody?

Flexi-Fluors are provided at a standard 0.2 mg/mL concentration. We recommend that you titrate your antibody to determine the optimal concentration to use for your application. For many flow cytometry applications, conjugated antibodies perform well at concentrations ranging from 0.03 to 1.0 µg per million cells in 100 µL volume. We recommend that you test a range of concentrations starting from 10 µg/mL.

For example, make five 1:1 serial dilutions of your 0.2 mg/mL antibody. Add 5 µL of each dilution (including the undiluted antibody) to 100 µL of cells (at 10⁷ cells/ml) to test six concentrations - 1.0, 0.5, 0.25, 0.125, 0.06, and 0.03 µg per million cells in 100 µL volume. Compare staining patterns or create a titration curve using the MFI or staining index to determine the optimal concentration.

I can’t find the antibody-dye combination that I need. When will it be available?

We continuously update our catalog, introducing scores of new products every month. Please get in touch with our Technical Service team for an update on new products or recommendations for suitable alternatives to complete your panel. Or contact Custom Solutions to inquire about our affordable custom conjugation services.

I need help to validate the performance of my Flexi-Fluor. Who should I contact?

Please get in touch with Technical Service for assistance.

Can I order more than 50 μg of a Flexi-Fluor?

Yes, you can order multiple vials of the same Flexi-Fluor products. We cannot guarantee, however, that these vials will be bottled from the same lot. For bulk single-lot orders, contact our Custom Solutions team.

What is the expiration date of my Flexi-Fluor?

Expiration dates can be found on the vial label or by using our CoA lookup tool.

Other Formats

View All CD90/CD90.1 Reagents Request Custom Conjugation

Description	Clone	Applications
PE/Cyanine7 anti-rat CD90/mouse CD90.1 (Thy1.1)	OX-7	FC
Purified anti-rat CD90/mouse CD90.1 (Thy1.1)	OX-7	FC,IHC-F,ICC,IHC-P,IP,WB
FITC anti-rat CD90/mouse CD90.1 (Thy1.1)	OX-7	FC
Alexa Fluor® 488 anti-rat CD90/mouse CD90.1 (Thy1.1)	OX-7	FC,IHC-F
Alexa Fluor® 647 anti-rat CD90/mouse CD90.1 (Thy1.1)	OX-7	FC,IHC-F
PerCP/Cyanine5.5 anti-rat CD90/mouse CD90.1 (Thy1.1)	OX-7	FC
APC/Cyanine7 anti-rat CD90/mouse CD90.1 (Thy1.1)	OX-7	FC
Pacific Blue™ anti-rat CD90/mouse CD90.1 (Thy1.1)	OX-7	FC
PerCP anti-rat CD90/mouse CD90.1 (Thy1.1)	OX-7	FC
PE anti-rat CD90/mouse CD90.1 (Thy1.1)	OX-7	FC
APC anti-rat CD90/mouse CD90.1 (Thy1.1)	OX-7	FC
Alexa Fluor® 700 anti-rat CD90/mouse CD90.1 (Thy1.1)	OX-7	FC
Biotin anti-rat CD90/mouse CD90.1 (Thy1.1)	OX-7	FC
Brilliant Violet 421™ anti-rat CD90/mouse CD90.1 (Thy1.1)	OX-7	FC
Brilliant Violet 650™ anti-rat CD90/mouse CD90.1 (Thy1.1)	OX-7	FC
Brilliant Violet 510™ anti-rat CD90/mouse CD90.1 (Thy1.1)	OX-7	FC
Brilliant Violet 605™ anti-rat CD90/mouse CD90.1 (Thy1.1)	OX-7	FC
Brilliant Violet 711™ anti-rat CD90/mouse CD90.1 (Thy1.1)	OX-7	FC
PE/Dazzle™ 594 anti-rat CD90/mouse CD90.1 (Thy1.1)	OX-7	FC
APC/Fire™ 750 anti-rat CD90/mouse CD90.1 (Thy1.1)	OX-7	FC
Alexa Fluor® 594 anti-rat CD90/mouse CD90.1 (Thy1.1)	OX-7	IHC-F
TotalSeq™-A0380 anti-rat CD90/mouse CD90.1 (Thy1.1)	OX-7	PG
TotalSeq™-B0380 anti-rat CD90/mouse CD90.1 (Thy1.1)	OX-7	PG
TotalSeq™-C0380 anti-rat CD90/mouse CD90.1 (Thy1.1)	OX-7	PG
Brilliant Violet 785™ anti-rat CD90/mouse CD90.1 (Thy1.1)	OX-7	FC
Spark Blue™ 550 anti-rat CD90/mouse CD90.1 (Thy1.1) (Flexi-Fluor™)	OX-7	FC
Spark Blue™ 574 anti-rat CD90/mouse CD90.1 (Thy1.1) (Flexi-Fluor™)	OX-7	FC
Spark Red™ 718 anti-rat CD90/mouse CD90.1 (Thy1.1) (Flexi-Fluor™)	OX-7	FC

Certificate of Analysis

Go To Top Version: 1 Revision Date: 01.17.2025

For Research Use Only. Not for diagnostic or therapeutic use.

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.