Spark NIR™ 685 anti-human CD206 (MMR) Antibody, CD206, 15-2

Pricing & Availability

Clone: 15-2 (See other available formats)
Regulatory Status: RUO
Other Names: MMR (macrophage mannose receptor), MR (mannose receptor), CD206, MRC1
Isotype: Mouse IgG1, κ
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15-2_Spark-NIR-685_CD206_Antibody_060723 — GM-CSF stimulated (3 days) human peripheral blood monocytes were stained with anti-human CD206 (MMR) (clone 15-2) Spark NIR™ 685 (filled histogram) or mouse IgG1, κ Spark NIR™ 685 isotype control (open histogram).

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321157

25 tests

100€

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321158

100 tests

212€

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Description

Macrophage mannose receptor (MMR) is a 162-175 kD type I membrane protein also known as CD206, MRC1, or mannose receptor (MR). It is a pattern recognition receptor (PRR) that belongs to C-type lectin superfamily. MMR is expressed on macrophages, dendritic cells, and hepatic or lymphatic endothelial cells, but not on monocytes. MMR recognizes a range of microbial carbohydrates bearing mannose, fucose, or N-acetyl glucosamine. MMR mediates endocytosis and phagocytosis, induces activation of macrophages and antigen presentation, plays an important role in host defense, and provides a link between innate and adaptive immunity.

Product Details

Technical Data Sheet (pdf)

Product Details

Verified Reactivity: Human
Antibody Type: Monoclonal
Host Species: Mouse
Immunogen: Purified human mannose receptor
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation: The antibody was purified by affinity chromatography and conjugated with Spark NIR™ 685 under optimal conditions.
Concentration: Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: FC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µL per million cells in 100 µL staining volume or 5 µL per 100 µL of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.

* Spark NIR™ 685 has a maximum excitation of 665 nm and a maximum emission of 685 nm.
Excitation Laser: Red Laser (633 nm)
Application Notes: The 15-2 antibody blocks the interaction of MMR with its ligand, and inhibits mannose receptor-mediated degradation of t-PA by macrophages. Additional reported applications of this antibody (for the relevant formats) include: Western blotting¹, blocking of ligand binding^1,2, immunofluorescence³, and immunohistochemical staining of acetone-fixed frozen tissue sections¹. The Utra-LEAF™ purified antibody (Endotoxin < 0.01 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (Cat. No. 321149 and 321150).
RRID: AB_2941490 (BioLegend Cat. No. 321157)
AB_2941490 (BioLegend Cat. No. 321158)

Antigen Details

Structure: Type I membrane protein, Pattern Recognition Receptor (PRR) family, C-type lectin superfamily, 162-175 kD
Distribution: Macrophages, dendritic cells, hepatic and lymphatic endothelial cells
Function: Pathogen binding, facilitate phagocytosis and endocytosis, macrophage activation and antigen presentation
Ligand/Receptor: Mannose, fucose, N-acetyl glucosamine
Cell Type: Dendritic cells, Endothelial cells, Macrophages
Biology Area: Cell Biology, Immunology, Neuroscience, Neuroscience Cell Markers
Molecular Family: CD Molecules
Antigen References: 1. Mason D, et al. Eds. 2002. Leukocyte Typing VII. Oxford University Press. p303
2. Wileman TE, et al. 1986. P. Natl. Acad. Sci. USA 83:2501.
3. Apostolopoulos V and McKenzie IF. 2001. Curr. Mol. Med. 1:469.
4. Le Cabec V, et al. 2005. J. Leukocyte Biol. 77:934.
5. Barrett-Bergshoeff M, et al. 1997. Thromb. Haemostatis 77:718.
Gene ID: 4360 View all products for this Gene ID
UniProt: View information about CD206 on UniProt.org

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Cell Surface Flow Cytometry Staining Protocol

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Other Formats

View All CD206 Reagents Request Custom Conjugation

Description	Clone	Applications
Purified anti-human CD206 (MMR)	15-2	FC,WB,ICC,IHC-F
FITC anti-human CD206 (MMR)	15-2	FC
PE anti-human CD206 (MMR)	15-2	FC
PE/Cyanine5 anti-human CD206 (MMR)	15-2	FC
APC anti-human CD206 (MMR)	15-2	FC
Alexa Fluor® 488 anti-human CD206 (MMR)	15-2	FC
Alexa Fluor® 647 anti-human CD206 (MMR)	15-2	FC
Biotin anti-human CD206 (MMR)	15-2	FC
APC/Cyanine7 anti-human CD206 (MMR)	15-2	FC
PerCP/Cyanine5.5 anti-human CD206 (MMR)	15-2	FC
PE/Cyanine7 anti-human CD206 (MMR)	15-2	FC
Brilliant Violet 421™ anti-human CD206 (MMR)	15-2	FC
Purified anti-human CD206 (MMR) (Maxpar® Ready)	15-2	FC,CyTOF®
Alexa Fluor® 700 anti-human CD206 (MMR)	15-2	FC
PE/Dazzle™ 594 anti-human CD206 (MMR)	15-2	FC
APC/Fire™ 750 anti-human CD206 (MMR)	15-2	FC
Brilliant Violet 711™ anti-human CD206 (MMR)	15-2	FC
Brilliant Violet 510™ anti-human CD206 (MMR)	15-2	FC
Brilliant Violet 605™ anti-human CD206 (MMR)	15-2	FC
Brilliant Violet 785™ anti-human CD206 (MMR)	15-2	FC
TotalSeq™-A0205 anti-human CD206 (MMR)	15-2	PG
TotalSeq™-B0205 anti-human CD206 (MMR)	15-2	PG
TotalSeq™-C0205 anti-human CD206 (MMR)	15-2	PG
Ultra-LEAF™ Purified anti-human CD206 (MMR)	15-2	FC,WB,ICC,IHC-F
Pacific Blue™ anti-human CD206 (MMR)	15-2	FC
PE/Fire™ 700 anti-human CD206 (MMR)	15-2	FC
TotalSeq™-D0205 anti-human CD206 (MMR)	15-2	PG
Spark NIR™ 685 anti-human CD206 (MMR)	15-2	FC
Spark Red™ 718 anti-human CD206 (MMR) (Flexi-Fluor™)	15-2	FC
Brilliant Violet 650™ anti-human CD206 (MMR)	15-2	FC
Spark Blue™ 574 anti-human CD206 (MMR) (Flexi-Fluor™)	15-2	FC
Spark Blue™ 550 anti-human CD206 (MMR) (Flexi-Fluor™)	15-2	FC

Certificate of Analysis

Go To Top Version: 1 Revision Date: 05.26.2023

For Research Use Only. Not for diagnostic or therapeutic use.

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

BioLegend, the BioLegend logo, and all other trademarks are property of BioLegend, Inc. or their respective owners, and all rights are reserved.

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Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
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