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Spark PLUS UV395™ anti-human CD31 Antibody

Pricing & Availability
Clone
W18200D (See other available formats)
Regulatory Status
RUO
Other Names
PECAM-1, EndoCAM
Isotype
Rat IgG2a, λ
Ave. Rating
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Product Citations
publications
W18200D_SparkPlusUV395_CD31_Antibody_1_112124
Human peripheral blood lymphocytes were stained with anti-human CD19 (clone HCD19) PerCP/Fire™ 806 and anti-human CD31 (clone W18200D) Spark PLUS UV395™ (left) or rat IgG2a, κ Spark PLUS UV395™ isotype control (right).
  • W18200D_SparkPlusUV395_CD31_Antibody_1_112124
    Human peripheral blood lymphocytes were stained with anti-human CD19 (clone HCD19) PerCP/Fire™ 806 and anti-human CD31 (clone W18200D) Spark PLUS UV395™ (left) or rat IgG2a, κ Spark PLUS UV395™ isotype control (right).
  • W18200D_SparkPlusUV395_CD31_Antibody_2_112124-2
    Human peripheral blood granulocytes were stained with anti-human CD31 (clone W18200D) Spark PLUS UV395™ (filled histogram) or rat IgG2a, κ Spark PLUS UV395™ isotype control (open histogram).
See Spark PLUS UV™ 395 spectral data
Cat # Size Price Quantity Check Availability Save
375903 25 tests 148€
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375904 100 tests 299€
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Description

CD31 is a 130-140 kD type I transmembrane glycoprotein also known as platelet endothelial cell adhesion molecule-1 (PECAM-1) or Endocam. It is expressed on monocytes, platelets, granulocytes, endothelial cells and lymphocyte subsets. CD31 has been reported to bind CD38 and be involved in wound healing, angiogenesis, and cellular migration in an inflammatory situation.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Recombinant human CD31 protein
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation
The antibody was purified by affinity chromatography and conjugated with Spark PLUS UV395™ under optimal conditions.
Concentration
Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µL per million cells in 100 µL staining volume or 5 µL per 100 µL of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.

* Spark PLUS UV395™ has a maximum excitation of 355 nm and a maximum emission of 385 nm.

Excitation Laser
Ultraviolet Laser (355 nm)
Application Notes

Clone W18200D antibody does not affect target binding of clone WM59 and vice versa.

Antigen Details

Structure
Ig superfamily, type I transmembrane glycoprotein, 130-140 kD
Distribution

Monocytes, platelets, granulocytes, endothelial cells, lymphocyte subset

Function
Cell adhesion, signal transduction
Ligand/Receptor
CD38
Cell Type
Endothelial cells, Granulocytes, Lymphocytes, Monocytes, Neutrophils, Platelets
Biology Area
Angiogenesis, Cell Adhesion, Cell Biology, Immunology, Neuroinflammation, Neuroscience
Molecular Family
Adhesion Molecules, CD Molecules
Antigen References

1. DeLisser H, et al. 1994. Immunol. Today 15:490.
2. Newman P, 1997. J. Clin. Invest. 99:3.
3. Fawcett J, et al. 1995. J. Cell Biol. 128:1229.

Gene ID
5175 View all products for this Gene ID
UniProt
View information about CD31 on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 1    Revision Date: 11.21.2024

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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