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- Clone
- S19004C (See other available formats)
- Regulatory Status
- RUO
- Other Names
- Killer Cell Lectin Like Receptor C1, KLRC1, NK Cell Receptor A
- Isotype
- Mouse IgG1, κ
- Ave. Rating
- Submit a Review
- Product Citations
- publications
CD159a, also known as NKG2A or KLRC1, is a type II transmembrane protein. It belongs to the killer cell lectin-like receptor family also known as the NKG2 family. It is expressed on NK and NKT cells and T cell subsets. NKG2A is an inhibitory NK cell receptor and it is expressed as a heterodimer with CD94. In human, NKG2A/CD94 complex interacts HLA-E on target cells and inhibits T cells and NK cell effector functions. Recent studies showed that NKG2A blockade enhances the anti-tumor effect of NK cell and CD8+ T cells.
Product DetailsProduct Details
- Verified Reactivity
- Human
- Antibody Type
- Monoclonal
- Host Species
- Mouse
- Immunogen
- Human NKG2A/CD94-transfectants
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
- Preparation
- The antibody was purified by affinity chromatography and conjugated with Spark Red™ 718 under optimal conditions.
- Concentration
- 0.2 mg/mL
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
- Application
-
FC
- Recommended Usage
-
Flexi-Fluors™ are provided at a standard 0.2 mg/mL concentration. We recommend titrating this reagent to determine the optimal concentration for each application. For many flow cytometry applications, conjugated antibodies perform well at concentrations ranging from 0.03 to 1.0 µg per million cells in 100 µL. We recommend testing a range of concentrations starting from 10 µg/mL.
For example, make five 1:1 serial dilutions of the 0.2 mg/mL antibody. Add 5 µL of each dilution (including the undiluted antibody) to 100 µL of cells (at 107 cells/mL) to test six concentrations -- 1.0, 0.5, 0.25, 0.125, 0.06, and 0.03 µg per million cells in 100 µL volume. Compare staining patterns or create a titration curve using the MFI or staining index to determine the optimal concentration. - Excitation Laser
-
Red Laser (633 nm)
- Application Notes
-
Clone S19004C does not cross-react with mouse CD159a.
- Additional Product Notes
-
For more information about Flexi-Fluors™, visit our Flexi-Fluor™ page and review FAQs associated with this product line.
- RRID
-
AB_3106358 (BioLegend Cat. No. 285106)
Antigen Details
- Structure
- Type II transmembrane proteins of the C-type lectin superfamily
- Distribution
-
NK cells, NKT cells, T cell subsets
- Function
- Inhibiting NK cell receptor
- Ligand/Receptor
- HLA-E
- Cell Type
- Lymphocytes, NK cells, NKT cells, T cells
- Biology Area
- Immuno-Oncology, Immunology
- Molecular Family
- Immune Checkpoint Receptors
- Antigen References
-
- Brooks AG, et al. 1999. J Immunol. 162:305-13.
- Kaiser BK, et al. 2008. Proc Natl Acad Sci U S A. 105: 6696-701.
- Braud VM, et al. 2003. Trends Immunol. 24:162-4.
- Kamiya T, et al. 2019. J Clin Invest. 129:2094.
- André P, et al. 2018. Cell. 175:1731.
- Gene ID
- 3821 View all products for this Gene ID
- UniProt
- View information about CD159a on UniProt.org
Related Pages & Pathways
Pathways
Related FAQs
- What are Flexi-Fluors?
-
Flexi-Fluors are rapidly made-to-order conjugated antibodies. The technology, manufacturing processes, and specifications used to create Flexi-Fluors are the same as our regular catalog products. However, the optimal concentration and performance of each Flexi-Fluor must be determined by the customer.
- How quickly will I receive my order?
-
We aim to ship Flexi-Fluors within 2-3 weeks of receipt of your order. However, depending on your location, shipping times may vary.
- How are Flexi-Fluors different from regular catalog products?
-
Flexi-Fluors are made on demand, specifically for you. Flexi-Fluors are manufactured using the same high-quality standards, and specifications as other catalog products. For faster delivery, Flexi-Fluors are not tested by flow cytometry to determine optimal concentrations or evaluate performance. This testing needs to be performed by the customer.
- How do I determine the optimal concentration for using my Flexi-Fluor? How should I titrate my antibody?
-
Flexi-Fluors are provided at a standard 0.2 mg/mL concentration. We recommend that you titrate your antibody to determine the optimal concentration to use for your application. For many flow cytometry applications, conjugated antibodies perform well at concentrations ranging from 0.03 to 1.0 µg per million cells in 100 µL volume. We recommend that you test a range of concentrations starting from 10 µg/mL.
For example, make five 1:1 serial dilutions of your 0.2 mg/mL antibody. Add 5 µL of each dilution (including the undiluted antibody) to 100 µL of cells (at 107 cells/ml) to test six concentrations - 1.0, 0.5, 0.25, 0.125, 0.06, and 0.03 µg per million cells in 100 µL volume. Compare staining patterns or create a titration curve using the MFI or staining index to determine the optimal concentration.
- I can’t find the antibody-dye combination that I need. When will it be available?
-
We continuously update our catalog, introducing scores of new products every month. Please get in touch with our Technical Service team for an update on new products or recommendations for suitable alternatives to complete your panel. Or contact Custom Solutions to inquire about our affordable custom conjugation services.
- I need help to validate the performance of my Flexi-Fluor. Who should I contact?
-
Please get in touch with Technical Service for assistance.
- Can I order more than 50 μg of a Flexi-Fluor?
-
Yes, you can order multiple vials of the same Flexi-Fluor products. We cannot guarantee, however, that these vials will be bottled from the same lot. For bulk single-lot orders, contact our Custom Solutions team.
- What is the expiration date of my Flexi-Fluor?
-
Expiration dates can be found on the vial label or by using our CoA lookup tool.
Other Formats
View All CD159a Reagents Request Custom ConjugationCompare Data Across All Formats
This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.
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Purified anti-human CD159a (NKG2A)
Human peripheral blood lymphocytes were stained with purifie... -
PE anti-human CD159a (NKG2A)
Human peripheral blood lymphocytes were stained with CD3 FIT... -
APC anti-human CD159a (NKG2A) Antibody
Human peripheral blood lymphocytes were stained with CD3 FIT... -
Pacific Blue™ anti-human CD159a (NKG2A) Antibody
Human peripheral blood lymphocytes were stained with CD3 APC... -
PE/Cyanine7 anti-human CD159a (NKG2A) Antibody
Human peripheral blood lymphocytes were stained with CD3 FIT... -
Alexa Fluor® 647 anti-human CD159a (NKG2A) Antibody
Human peripheral blood lymphocytes were stained with CD3 FIT... -
PE/Cyanine5 anti-human CD159a (NKG2A)
Human peripheral blood lymphocytes were stained with CD3 FIT... -
APC/Fire™ 750 anti-human CD159a (NKG2A)
Human peripheral blood lymphocytes were stained with CD3 FIT... -
Alexa Fluor® 700 anti-human CD159a (NKG2A) Antibody
Human peripheral blood lymphocytes were stained with CD3 FIT... -
PE/Dazzle™ 594 anti-human CD159a (NKG2A) Antibody
Human peripheral blood lymphocytes were stained with CD3 APC... -
Biotin anti-human CD159a (NKG2A) Antibody
Human peripheral blood lymphocytes were stained with CD3 APC... -
Alexa Fluor® 488 anti-human CD159a (NKG2A)
Human peripheral blood lymphocytes were stained with anti-hu... -
PerCP/Cyanine5.5 anti-human CD159a (NKG2A)
Human peripheral blood lymphocytes were stained with anti-hu... -
FITC anti-human CD159a (NKG2A)
Human peripheral blood lymphocytes were stained with anti-hu... -
TotalSeq™-C1374 anti-human CD159a (NKG2A)
-
TotalSeq™-D1374 anti-human CD159a (NKG2A)
-
TotalSeq™-A1374 anti-human CD159a (NKG2A)
-
APC/Fire™ 810 anti-human CD159a (NKG2A)
Human peripheral blood lymphocytes were surface stained with... -
Brilliant Violet 421™ anti-human CD159a (NKG2A)
Human peripheral blood lymphocytes were surface stained with... -
Brilliant Violet 605™ anti-human CD159a (NKG2A)
Human peripheral blood lymphocytes were surface stained with... -
Brilliant Violet 785™ anti-human CD159a (NKG2A)
Human peripheral blood lymphocytes were stained with anti-hu... -
TotalSeq™-B1374 anti-human CD159a (NKG2A)
-
PerCP/Fire™ 806 anti-human CD159a (NKG2A)
Human peripheral blood lymphocytes were stained with anti-hu... -
PerCP/Fire™ 780 anti-human CD159a (NKG2A)
Human peripheral blood lymphocytes were stained with anti-hu... -
PE/Fire™ 810 anti-human CD159a (NKG2A)
Human peripheral blood lymphocytes were stained with anti-hu... -
APC/Cyanine7 anti-human CD159a (NKG2A)
Human peripheral blood lymphocytes were stained with anti-hu... -
Brilliant Violet 650™ anti-human CD159a (NKG2A)
Human peripheral blood lymphocytes were stained with anti-hu... -
Spark Red™ 718 anti-human CD159a (NKG2A) (Flexi-Fluor™)
-
Brilliant Violet 711™ anti-human CD159a (NKG2A)
Human peripheral blood lymphocytes were stained with anti-hu...
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