Spark Red™ 718 anti-human CD197 (CCR7) Antibody, CD197, G043H7

Pricing & Availability

Clone: G043H7 (See other available formats)
Regulatory Status: RUO
Other Names: BLR2, CDw197, EBI1, CMKBR7
Isotype: Mouse IgG2a, κ
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G043H7_SparkRed718_CD197_Antibody_1_052224 — Human peripheral blood lymphocytes were stained with anti-human CD3 (clone UCHT1) PE/Dazzle™ 594 and anti-human CD197 (CCR7) (clone G043H7) Spark Red™ 718 (left) or mouse IgG2a, κ Spark Red™ 718 isotype control (right).

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353277

25 tests

120€

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353278

100 tests

248€

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Description

CCR7, also known as CD197, is a chemokine receptor that binds CCL19 and CCL21. CCR7 and its ligands link innate and adaptive immunity by affecting interactions between T cells and dendritic cells and their downstream effect. Naïve T cells enter the lymph node through high endothelial venules, which express CCL21. Dendritic cells and macrophages enter the lymph node through afferent lymphatics. The encounter of T cells and dendritic cells in the T cell zone is CCR7-dependent. In addition, during immunological surveillance, B cells recirculate between B-cell-rich compartments (follicles or B cell zones) in secondary lymphoid organs, surveying for antigen. After antigen binding, B cells move to the boundary of B and T zones to interact with T-helper cells; this B cell migration is directed by CCR7 and its ligands. CCR7-positive cancer cell expression has been associated with lymph node metastasis.

Product Details

Technical Data Sheet (pdf)

Product Details

Verified Reactivity: Human
Reported Reactivity: African Green, Baboon, Cynomolgus, Rhesus
Antibody Type: Monoclonal
Host Species: Mouse
Immunogen: CCR7-transfected cells
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation: The antibody was purified by affinity chromatography and conjugated with Spark Red™ 718 under optimal conditions.
Concentration: Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: FC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µL per million cells in 100 µL staining volume or 5 µL per 100 µL of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.

* Spark Red™ 718 has a maximum excitation of 697 nm and a maximum emission of 718 nm.
Excitation Laser: Red Laser (633 nm)
RRID: AB_3106207 (BioLegend Cat. No. 353277)
AB_3106207 (BioLegend Cat. No. 353278)

Antigen Details

Structure: Chemokine receptor, G protein-coupled receptors (GPCR), seven transmembrane receptor.
Distribution: T cells, B cells, NK, dendritic cells.
Function: The chemokine receptor CCR7 plays a pivotal role in the homing of naïve T cells and regulatory T cells to secondary lymphoid organs, and the migration of dendritic cells into afferent lymphatic vessels.
Ligand/Receptor: CCL19 and CCL21.
Cell Type: B cells, Dendritic cells, NK cells, T cells
Biology Area: Immunology
Molecular Family: CD Molecules, Cytokine/Chemokine Receptors, GPCR
Antigen References: 1. Yanagihara S, et al. 1998. J. Immunol. 161:3096.
2. Charo IF, et al. 2006. N. Engl. J. Med. 354:610.
3. Reif K, et al. 2002. Nature 416:94.
4. Nakata B, et al. 2008. Oncology 74:69.
5. Brodie T. et al. 2013. Cytometry A. 6: 530-2. PubMed
6. Graves A.J. et al. 2014. Cytometry A. 7: 576–9 PubMed
7. Moncunill G. et al. 2014. Cytometry A. 12: 995-8 PubMed
Gene ID: 1236 View all products for this Gene ID
UniProt: View information about CD197 on UniProt.org

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Related Protocols

Cell Surface Flow Cytometry Staining Protocol

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Related Pages & Pathways

Pathways

Related FAQs

Does staining at room temperature or even at 37°C help for checking chemokine receptors expression?: Due to continuous recycling of many chemokine receptors, it may be worthwhile to consider staining at room temperature or at 37°C if the staining at lower temperature (which can potentially reduce receptor turnover) is not optimal.

Other Formats

View All CD197 Reagents Request Custom Conjugation

Description	Clone	Applications
Purified anti-human CD197 (CCR7)	G043H7	FC
Alexa Fluor® 488 anti-human CD197 (CCR7)	G043H7	FC
Brilliant Violet 421™ anti-human CD197 (CCR7)	G043H7	FC
PE anti-human CD197 (CCR7)	G043H7	FC
APC/Cyanine7 anti-human CD197 (CCR7)	G043H7	FC
Pacific Blue™ anti-human CD197 (CCR7)	G043H7	FC
APC anti-human CD197 (CCR7)	G043H7	FC
FITC anti-human CD197 (CCR7)	G043H7	FC
Alexa Fluor® 647 anti-human CD197 (CCR7)	G043H7	FC
PerCP/Cyanine5.5 anti-human CD197 (CCR7)	G043H7	FC
Brilliant Violet 605™ anti-human CD197 (CCR7)	G043H7	FC
PE/Cyanine7 anti-human CD197 (CCR7)	G043H7	FC
Brilliant Violet 711™ anti-human CD197 (CCR7)	G043H7	FC
Brilliant Violet 785™ anti-human CD197 (CCR7)	G043H7	FC
Brilliant Violet 510™ anti-human CD197 (CCR7)	G043H7	FC
Brilliant Violet 650™ anti-human CD197 (CCR7)	G043H7	FC
PE/Dazzle™ 594 anti-human CD197 (CCR7)	G043H7	FC
Biotin anti-human CD197 (CCR7)	G043H7	FC
Purified anti-human CD197 (CCR7) (Maxpar® Ready)	G043H7	FC,CyTOF®
PerCP anti-human CD197 (CCR7)	G043H7	FC
Alexa Fluor® 700 anti-human CD197 (CCR7)	G043H7	FC
APC/Fire™ 750 anti-human CD197 (CCR7)	G043H7	FC
TotalSeq™-A0148 anti-human CD197 (CCR7)	G043H7	PG
TotalSeq™-B0148 anti-human CD197 (CCR7)	G043H7	PG
TotalSeq™-C0148 anti-human CD197 (CCR7)	G043H7	PG
Brilliant Violet 750™ anti-human CD197 (CCR7)	G043H7	FC
Ultra-LEAF™ Purified anti-human CD197 (CCR7)	G043H7	FC,Block
Spark NIR™ 685 anti-human CD197 (CCR7)	G043H7	FC
KIRAVIA Blue 520™ anti-human CD197 (CCR7)	G043H7	FC
PE/Fire™ 640 anti-human CD197 (CCR7)	G043H7	FC
Spark YG™ 581 anti-human CD197 (CCR7)	G043H7	FC
APC/Fire™ 810 anti-human CD197 (CCR7) Antibody	G043H7	FC
TotalSeq™-D0148 anti-human CD197 (CCR7)	G043H7	PG
PE/Fire™ 810 anti-human CD197 (CCR7) Antibody	G043H7	FC
PE/Cyanine5 anti-human CD197 (CCR7)	G043H7	FC
Spark YG™ 593 anti-human CD197 (CCR7)	G043H7	FC
PE/Fire™ 744 anti-human CD197 (CCR7)	G043H7	FC
Spark Red™ 718 anti-human CD197 (CCR7) (Flexi-Fluor™)	G043H7	FC
Spark Red™ 718 anti-human CD197 (CCR7)	G043H7	FC
PE anti-human CD197	G043H7	FC
Spark PLUS UV395™ anti-human CD197 (CCR7)	G043H7	FC

Certificate of Analysis

Go To Top Version: 1 Revision Date: 05.22.2024

For Research Use Only. Not for diagnostic or therapeutic use.

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

BioLegend, the BioLegend logo, and all other trademarks are property of BioLegend, Inc. or their respective owners, and all rights are reserved.

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