Spark YG™ 593 anti-human CD4 antibody, CD4, SK3

Pricing & Availability

Clone: SK3 (See other available formats)
Regulatory Status: RUO
Other Names: T4, Leu3a
Isotype: Mouse IgG1, κ
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Product Citations: publications

Human peripheral blood lymphocytes were stained with anti-human CD3 FITC and anti-human CD4 (clone SK3) Spark YG™ 593 (left) or cells stained only with anti-human CD3 FITC (right).

Compare all formats See Spark YG™ 593 spectral data

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344671

25 tests

90€

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344672

100 tests

224€

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Description

CD4, also known as T4, is a 55 kD single-chain type I transmembrane glycoprotein expressed on most thymocytes, a subset of T cells, and monocytes/macrophages. CD4, a member of the Ig superfamily, recognizes antigens associated with MHC class II molecules and participates in cell-cell interactions, thymic differentiation, and signal transduction. CD4 acts as a primary receptor for HIV, binding to HIV gp120. CD4 has also been shown to interact with IL-16.

Product Details

Technical Data Sheet (pdf)

Product Details

Verified Reactivity: Human
Antibody Type: Monoclonal
Host Species: Mouse
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation: The antibody was purified by affinity chromatography and conjugated with Spark YG™ 593 under optimal conditions.
Concentration: Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: FC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µL per million cells in 100 µL staining volume or 5 µL per 100 µL of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.

* Spark YG™ 593 has a maximum excitation of 573 nm and a maximum emission of 593 nm.
Excitation Laser: Green Laser (532 nm)/Yellow-Green Laser (561 nm)
RRID: AB_2894479 (BioLegend Cat. No. 344671)
AB_2894479 (BioLegend Cat. No. 344672)

Antigen Details

Structure: Ig superfamily, type I transmembrane glycoprotein, 55 kD
Distribution: T cell subset, majority of thymocytes, monocytes/macrophages
Function: MHC class II co-receptor, lymphocyte adhesion, thymic differentiation, HIV receptor
Ligand/Receptor: MHC class II molecules, HIV gp120, IL-16
Cell Type: Macrophages, Monocytes, T cells, Thymocytes, Tregs
Biology Area: Immunology
Molecular Family: CD Molecules
Antigen References: 1. Center D et al. 1996. Immunol. Today 17:476.
2. Gaubin M et al. 1996. Eur. J. Clin. Chem. Clin. Biochem. 34:723.
Gene ID: 920 View all products for this Gene ID
UniProt: View information about CD4 on UniProt.org

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Cell Surface Flow Cytometry Staining Protocol

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PerCP anti-human CD4	SK3	FC
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FITC anti-human CD4	SK3	FC
PE anti-human CD4	SK3	FC
PerCP/Cyanine5.5 anti-human CD4	SK3	FC
Biotin anti-human CD4	SK3	FC
PE/Cyanine7 anti-human CD4	SK3	FC
APC anti-human CD4	SK3	FC
APC/Cyanine7 anti-human CD4	SK3	FC
Alexa Fluor® 488 anti-human CD4	SK3	FC
Pacific Blue™ anti-human CD4	SK3	FC
Alexa Fluor® 700 anti-human CD4	SK3	FC
Purified anti-human CD4 (Maxpar® Ready)	SK3	FC,CyTOF®
PE anti-human CD4	SK3	FC
Brilliant Violet 421™ anti-human CD4	SK3	FC
Brilliant Violet 510™ anti-human CD4	SK3	FC
Alexa Fluor® 647 anti-human CD4	SK3	FC
FITC anti-human CD4	SK3	FC
APC/Fire™ 750 anti-human CD4	SK3	FC
PE/Dazzle™ 594 anti-human CD4	SK3	FC
Pacific Blue™ anti-human CD4	SK3	FC
Brilliant Violet 785™ anti-human CD4	SK3	FC
Brilliant Violet 750™ anti-human CD4	SK3	FC
Brilliant Violet 605™ anti-human CD4	SK3	FC
Brilliant Violet 711™ anti-human CD4	SK3	FC
PE/Cyanine7 anti-human CD4	SK3	FC
TotalSeq™-A0045 anti-human CD4	SK3	PG
TotalSeq™-C0045 anti-human CD4	SK3	PG
PE/Cyanine5 anti-human CD4	SK3	FC
PerCP/Cyanine5.5 anti-human CD4	SK3	FC
Spark Blue™ 550 anti-human CD4	SK3	FC
Spark NIR™ 685 anti-human CD4	SK3	FC
APC anti-human CD4	SK3	FC
KIRAVIA Blue 520™ anti-human CD4	SK3	FC
APC/Fire™ 810 anti-human CD4	SK3	FC
PE/Fire™ 640 anti-human CD4	SK3	FC
PE/Fire™ 700 anti-human CD4	SK3	FC
PerCP anti-human CD4	SK3	FC
Spark Violet™ 538 anti-human CD4 Antibody	SK3	FC
Spark YG™ 581 anti-human CD4	SK3	FC
Alexa Fluor® 660 anti-human CD4	SK3	FC
Spark YG™ 593 anti-human CD4	SK3	FC
PE/Fire™ 810 anti-human CD4 Antibody	SK3	FC
APC/Fire™ 750 anti-human CD4	SK3	FC
Spark Blue™ 574 anti-human CD4	SK3	FC
GMP APC anti-human CD4	SK3	FC
TotalSeq™-B0045 anti-human CD4 Antibody	SK3	PG
Spark Violet™ 423 anti-human CD4 Antibody	SK3	FC
GMP Pacific Blue™ anti-human CD4	SK3	FC
Spark UV™ 387 anti-human CD4	SK3	FC
GMP PE anti-human CD4	SK3	FC
GMP FITC anti-human CD4	SK3	FC
Spark Red™ 718 anti-human CD4	SK3	FC
GMP PerCP anti-human CD4	SK3	FC
GMP PE/Cyanine7 anti-human CD4	SK3	FC
Spark Violet™ 500 anti-human CD4	SK3	FC
GMP PerCP/Cyanine5.5 anti-human CD4	SK3	FC
Brilliant Violet 650™ anti-human CD4	SK3	FC
PerCP/Fire™ 806 anti-human CD4	SK3	FC
Spark Blue™ 515 anti-human CD4	SK3	FC
GMP APC/Fire™ 750 anti-human CD4	SK3	FC
Spark Violet™ 423 anti-human CD4	SK3	FC
PerCP/Fire™ 780 anti-human CD4	SK3	FC
PE/Fire™ 744 anti-human CD4	SK3	FC
Spark PLUS UV395™ anti-human CD4	SK3	FC
Spark PLUS B550™ anti-human CD4	SK3	FC

Certificate of Analysis

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Go To Top Version: 1 Revision Date: 06.10.2021

For Research Use Only. Not for diagnostic or therapeutic use.

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Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
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