TotalSeq-D0120 anti-mouse TCR β chain Antibody, TCR β chain, H57-597

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Clone: H57-597 (See other available formats)
Regulatory Status: RUO
Other Names: TCR-β chain, TCR-β, β-TCR
Isotype: Armenian Hamster IgG
Barcode Sequence: TCCTATGGGACTCAG
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Product Citations: publications

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109265

10 µg

296€

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Description

T cell receptor (TCR) is a heterodimer consisting of an α and a β chain (TCR α/β) or a γ and a δ chain (TCR γ/δ). TCR-β is a member of the immunoglobulin superfamily and a component of the CD3/TCR complex (along with TCR-α). It is expressed on α/β TCR-bearing T cells and thymocytes. The CD3/TCR complex plays a key role in antigen recognition, signal transduction, and T cell activation.

Product Details

Technical Data Sheet (pdf)

Product Details

Verified Reactivity: Mouse
Antibody Type: Monoclonal
Host Species: Armenian Hamster
Immunogen: Affinity purified TCR from mouse DO-11.10 cells
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and EDTA
Preparation: The antibody was purified by chromatography and conjugated with TotalSeq™-D oligomer under optimal conditions.
Concentration: 0.5 mg/mL
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C. Do not freeze.
Application: PG - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis and the oligomer sequence is confirmed by sequencing. TotalSeq™-D antibodies are compatible with Mission Bio’s Tapestri Single-Cell Sequencing Platform for simultaneous detection of DNA and Protein. To maximize performance, it is strongly recommended that the reagent be titrated for each application, and that you centrifuge the antibody dilution before adding to the cells at 14,000xg at 2 - 8°C for 10 minutes. Carefully pipette out the liquid avoiding the bottom of the tube and add to the cell suspension. For Proteogenomics analysis, the suggested starting amount of this reagent for titration is ≤ 1.0 µg per million cells in 100 µL volume. Refer to the corresponding TotalSeq™ protocol for specific staining instructions.

Buyer is solely responsible for determining whether Buyer has all intellectual property rights that are necessary for Buyer's intended uses of the BioLegend TotalSeq™ products. For example, for any technology platform Buyer uses with TotalSeq™, it is Buyer's sole responsibility to determine whether it has all necessary third party intellectual property rights to use that platform and TotalSeq™ with that platform.
Application Notes: H57-597 is a hamster mAb directed to an epitope of the C region of TCR ß chain¹². The H57-597 antibody does not cross-react with ?/d TCR-bearing T cells. Immobilized or soluble H57-597 antibody can activate a/ß TCR-bearing T cells. Additional reported applications (for the relevant formats) for this antibody include: immunoprecipitation², in vitro stimulation^2,3, in vivo depletion^4-6, and immunohistochemical staining of acetone-fixed frozen sections^7,8,9. The Ultra-LEAF™ purified antibody (Endotoxin <0.01 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (Cat. No. 109253-109258).
Additional Product Notes: TotalSeq™-D reagents are designed to profile protein expression at single cell level. The Mission Bio Tapestri platform and sequencer (e.g. Illumina analyzers) are required. Please contact technical support for more information, or visit biolegend.com/totalseq/single-cell-dna

The barcode flanking sequences are CGAGATGACTACGCTACTCATGG (PCR handle), and GAGCCGATCTAGTATCTCAGT*C*G (capture sequence). * indicates a phosphorothioated bond, to prevent nuclease degradation.

View more applications data for this product in our Application Technical Notes.

Antigen Details

Structure: Ig superfamily, CD3/TCR complex with CD3 and TCR α subunit
Distribution: Majority of T cells and thymocytes (correlated to differentiation)
Function: Antigen recognition, T cell activation
Ligand/Receptor: Peptide bound-MHC class I and II
Antigen References: 1. Davis MM, et al. 1998. Ann. Rev. Immunol. 16:523.
2. Huppa JB, et al. 2003. Nat. Immunol. 4:749.
3. Kubo R, et al. 1989. J. Immunol. 142:2736.
Gene ID: 21577 View all products for this Gene ID
UniProt: View information about TCR beta chain on UniProt.org

Documentation

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Related Protocols

TotalSeq™-D with Mission Bio Tapestri® Single-Cell DNA + Protein Protocol

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Other Formats

View All TCR β chain Reagents Request Custom Conjugation

Description	Clone	Applications
APC anti-mouse TCR β chain	H57-597	FC
Biotin anti-mouse TCR β chain	H57-597	FC,IHC-F
FITC anti-mouse TCR β chain	H57-597	FC,IHC-F
PE anti-mouse TCR β chain	H57-597	FC
PE/Cyanine5 anti-mouse TCR β chain	H57-597	FC
Purified anti-mouse TCR β chain	H57-597	FC,CyTOF®,IHC-F,IP,Costim,Depletion
Alexa Fluor® 488 anti-mouse TCR β chain	H57-597	FC,IHC-F
Alexa Fluor® 647 anti-mouse TCR β chain	H57-597	FC,IHC-F
APC/Cyanine7 anti-mouse TCR β chain	H57-597	FC
PE/Cyanine7 anti-mouse TCR β chain	H57-597	FC
Alexa Fluor® 700 anti-mouse TCR β chain	H57-597	FC
Pacific Blue™ anti-mouse TCR β chain	H57-597	FC
Brilliant Violet 421™ anti-mouse TCR β chain	H57-597	FC,IHC-F
PerCP/Cyanine5.5 anti-mouse TCR β chain	H57-597	FC
Brilliant Violet 570™ anti-mouse TCR β chain	H57-597	FC
Brilliant Violet 510™ anti-mouse TCR β chain	H57-597	FC,IHC-F
Purified anti-mouse TCR β chain (Maxpar® Ready)	H57-597	FC,CyTOF®
Alexa Fluor® 594 anti-mouse TCR β chain	H57-597	FC,IHC-F
PE/Dazzle™ 594 anti-mouse TCR β chain	H57-597	FC
Brilliant Violet 605™ anti-mouse TCR β chain	H57-597	FC
Brilliant Violet 711™ anti-mouse TCR β chain	H57-597	FC
APC/Fire™ 750 anti-mouse TCR β chain	H57-597	FC
TotalSeq™-A0120 anti-mouse TCR β chain	H57-597	PG
Brilliant Violet 785™ anti-mouse TCR β chain	H57-597	FC
Brilliant Violet 650™ anti-mouse TCR β chain	H57-597	FC
Ultra-LEAF™ Purified anti-mouse TCR β chain	H57-597	FC,CyTOF®,IHC-F,IP,Costim,Depletion
TotalSeq™-C0120 anti-mouse TCR β chain	H57-597	PG
TotalSeq™-B0120 anti-mouse TCR β chain	H57-597	PG
Spark Red™ 718 anti-mouse TCR β chain (Flexi-Fluor™)	H57-597	FC
Spark PLUS UV395™ anti-mouse TCR β chain	H57-597	FC
TotalSeq™-D0120 anti-mouse TCR β chain	H57-597	PG
Spark Blue™ 574 anti-mouse TCR β chain (Flexi-Fluor™)	H57-597	FC
Spark Blue™ 550 anti-mouse TCR β chain (Flexi-Fluor™)	H57-597	FC

Certificate of Analysis

Go To Top Version: 1 Revision Date: 09.12.2024

For Research Use Only. Not for diagnostic or therapeutic use.

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

BioLegend, the BioLegend logo, and all other trademarks are property of BioLegend, Inc. or their respective owners, and all rights are reserved.

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
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