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True-Stain™ Multi-Fluor Buffer

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True-Stain-Multi-Fluor-Buffer_091724
Human peripheral blood lymphocytes were stained with anti-human CD4 (clone SK3) Brilliant Violet 421™ and anti-human CD8 (clone SK1) Brilliant Violet 605™ with (right) or without (left) True-Stain™ Multi-Fluor Buffer. Red circles indicate instances of staining artifacts caused by polymer dye interactions.
  • True-Stain-Multi-Fluor-Buffer_091724
    Human peripheral blood lymphocytes were stained with anti-human CD4 (clone SK3) Brilliant Violet 421™ and anti-human CD8 (clone SK1) Brilliant Violet 605™ with (right) or without (left) True-Stain™ Multi-Fluor Buffer. Red circles indicate instances of staining artifacts caused by polymer dye interactions.
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426105 100 tests 100€
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426106 1000 tests 512€
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Description

True-Stain™ Multi-Fluor Buffer helps prevent interactions between fluorescent reagents when staining cells with multiple Brilliant Violet™ or Brilliant Ultraviolet™ dyes. Fluorescent dye interactions can cause staining artifacts which may affect data interpretation. Use True-Stain™ Multi-Fluor Buffer when combining multiple Brilliant™ polymer dyes to minimize possible nonspecific interactions and increase confidence in your results.

This buffer is compatible with the use of conjugates of traditional fluorochromes and tandems, as well as Alexa Fluor®, Spark™, and Fire™ dyes. It is also compatible with commonly used BioLegend flow cytometry reagents and protocols, including fixation and permeabilization reagents.

True-Stain™ Multi-Fluor Buffer can be used with BioLegend Compensation Beads. It has not been tested for compatibility with compensation particles from other vendors.

Product Details
Technical Data Sheet (pdf)

Product Details

Formulation
True-Stain™ Multi-Fluor Buffer is supplied as a ready-to-use buffer for immunofluorescent staining of cells.
Storage & Handling
Store between 2°C and 8°C, and protected from prolonged exposure to light.
Application

FC - Quality tested
ICFC - Verified
Recommended Usage

Use 15 μL per tube or well per 100 µL of cells

Application Notes
True-Stain™ Multi-Fluor Buffer can be used for surface and intracellular staining of human or mouse cells.
 
Protocol
  1. Add 15 μL* of True-Stain™ Multi-Fluor Buffer to each empty tube/well.
  2. Add the recommended amount of each fluorescent reagent one by one. If needed, add Cell Staining Buffer (Cat. No. 420201) to increase the volume in each tube/well to 50 μL.
  3. Add 100 μL of cells.
  4. Follow standard staining protocol appropriate to tissue type as usual (see Related Protocols).​

​​​*Note: For final staining volumes greater than 150 μL, use True-Stain™ Multi-Fluor Buffer as a 10X solution.  For example, for a final volume of 200 μL, use 20 μL of True-Stain™ Multi-Fluor Buffer.

Antigen Details

Gene ID
NA
Go To Top Version: 1    Revision Date: 08.23.2024

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

BioLegend, the BioLegend logo, and all other trademarks are property of BioLegend, Inc. or their respective owners, and all rights are reserved.

 

8999 BioLegend Way, San Diego, CA 92121 www.biolegend.com
Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

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