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Ultra-LEAF™ Purified anti-human CD134 (OX40) Antibody

Pricing & Availability
Clone
W22033K (See other available formats)
Regulatory Status
RUO
Other Names
Tumor necrosis factor receptor superfamily member 4, TNFRSF4, ACT35, OX40L receptor, TAX transcriptionally-activated glycoprotein 1 receptor, CD134
Isotype
Rat IgG2b, κ
Ave. Rating
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Product Citations
publications
W22033K_ULEAF_CD234_Antibody_022624
Recombinant human OX40/TNFRSF4-Fc chimera (Cat. No. 780804) (black circles) binds to immobilized recombinant human OX40L (Cat. No. 555706). Ultra-LEAF™ purified anti-human CD134 (OX40) (clone W22033K) (purple squares) inhibits the binding in a dose-dependent manner, whereas the Ultra-LEAF™ purified rat IgG2b, κ isotype control (Cat. No. 400644) (gray triangles) does not have the effect. This antibody blocks the binding of 0.05 µg/mL OX40/TNFRSF4 to immobilized 1 µg/mL recombinant human OX40L. ND50 range: 4 - 24 ng/mL.
  • W22033K_ULEAF_CD234_Antibody_022624
    Recombinant human OX40/TNFRSF4-Fc chimera (Cat. No. 780804) (black circles) binds to immobilized recombinant human OX40L (Cat. No. 555706). Ultra-LEAF™ purified anti-human CD134 (OX40) (clone W22033K) (purple squares) inhibits the binding in a dose-dependent manner, whereas the Ultra-LEAF™ purified rat IgG2b, κ isotype control (Cat. No. 400644) (gray triangles) does not have the effect. This antibody blocks the binding of 0.05 µg/mL OX40/TNFRSF4 to immobilized 1 µg/mL recombinant human OX40L. ND50 range: 4 - 24 ng/mL.
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632653 100 µg 300€
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632654 1 mg 756€
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Select size of product is eligible for a 40% discount! Promotion valid until December 31, 2024. Exclusions apply. To view full promotion terms and conditions or to contact your local BioLegend representative to receive a quote, visit our webpage.

Description

OX40 (ACT35, CD134, TNFRSF4) is an approximately 48 kD transmembrane protein belonging to the TNF receptor superfamily. It is a 249 amino acid glycoprotein with a 41 amino acid cytoplasmic tail, 20 amino acid transmembrane domain, and a 185 amino acid extracellular domain. OX40 is a costimulatory molecule implicated in long-term T cell immunity. It is a receptor for TNFSF4, OX40L, GP34, and also acts as a receptor for human herpesvirus 6B/HHV-6B. OX40 is primarily expressed on activated T cells, including CD4 and CD8 T cells, Th1, Th2, and Th17 cells, and regulatory T cells. The main source of OX40L is antigen presenting cells. OX40L can also be induced on activated B cells, mature dendritic cells, Langerhans cells, and macrophages. OX40 and OX40L interactions regulate the division and survival of T cells. It inhibits the conversion of effector T cells into regulatory T cells (Tregs) and promote the recall response in memory T cells. OX40 and OX40L interactions are also involved in the development of multiple diseases including allergic airway inflammation, graft-versus-host disease and autoimmune disease.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Recombinant human OX40/TNFRSF4-Fc
Formulation
0.2 µm filtered in phosphate-buffered solution, pH 7.2, containing no preservative.
Endotoxin Level
< 0.01 EU/μg of the protein (< 0.001 ng/μg of the protein) as determined by the LAL test
Preparation
The Ultra-LEAF™ (Low Endotoxin, Azide-Free) antibody was purified by affinity chromatography.
Concentration
The antibody is bottled at the concentration indicated on the vial, typically between 2 mg/mL and 3 mg/mL. To obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C. This Ultra-LEAF™ solution contains no preservative; handle under aseptic conditions.
Application

Block - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by blocking the binding of 0.05 µg/mL recombinant human OX40/TNFRSF4-Fc chimera (Cat. No. 780804) to immobilized 1 µg/mL recombinant human OX40L (Cat. No. 555706).  ND50 = 4 - 24 ng/mL. It is recommended that the reagent be titrated for optimal performance for each application.

RRID
AB_3097584 (BioLegend Cat. No. 632653)
AB_3097584 (BioLegend Cat. No. 632654)

Antigen Details

Structure
Disulfide linked homodimer
Distribution

Primarily expressed on the activated T cells, including CD4 and CD8 T cells, Th1, Th2, and Th17 cells, and regulatory T cells

Function
Is a costimulatory molecule implicated in long-term T-cell immunity
Interaction
Antigen presenting cells
Ligand/Receptor
TNFSF4, OX40L, GP34, human herpesvirus 6B/HHV-6B
Cell Type
T cells, Tregs
Biology Area
Apoptosis/Tumor Suppressors/Cell Death, Cell Biology, Costimulatory Molecules, Immunology
Molecular Family
CD Molecules, Immune Checkpoint Receptors
Antigen References
  1. Baum PR, et al. 1994. Circ Shock. 44:30-4.
  2. Bansal-Pakala P, et al. 2001. Nat Med. 7:907-12.
  3. Humphreys IR, et al. 2003. J Exp Med. 198:1237-42.
  4. Shimojima M, et al. 2004. Science. 303:1192-5.
  5. Tang H, et al. 2013. Proc Natl Acad Sci USA. 110:9096-9.
  6. Jacquemin C, et al. 2015. Immunity. 42:1159-70.
Gene ID
7293 View all products for this Gene ID
UniProt
View information about CD134 on UniProt.org

Related FAQs

Do you guarantee that your antibodies are totally pathogen free?

BioLegend does not test for pathogens in-house aside from the GoInVivo™ product line. However, upon request, this can be tested on a custom basis with an outside, independent laboratory.

Does BioLegend test each Ultra-LEAF™ antibody by functional assay?

No, BioLegend does not test Ultra-LEAF™ antibodies by functional assays unless otherwise indicated. Due to the possible complexities and variations of uses of biofunctional antibodies in different assays and because of the large product portfolio, BioLegend does not currently perform functional assays as a routine QC for the antibodies. However, we do provide references in which the antibodies were used for functional assays and we do perform QC to verify the specificity and quality of the antibody based on our strict specification criteria.

Does BioLegend test each Ultra-LEAF™ antibody for potential pathogens?

No, BioLegend does not test for pathogens in-house unless otherwise indicated.  However, we can recommend an outside vendor to perform this testing as needed.

Have you tested this Ultra-LEAF™ antibody for in vivo or in vitro applications?

We don't test our antibodies for in vivo or in vitro applications unless otherwise indicated. Depending on the product, the TDS may describe literature supporting usage of a particular product for bioassay. It may be best to further consult the literature to find clone specific information.

Go To Top Version: 1    Revision Date: 02.26.2024

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

BioLegend, the BioLegend logo, and all other trademarks are property of BioLegend, Inc. or their respective owners, and all rights are reserved.

 

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Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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