- Clone
- 621 (See other available formats)
- Regulatory Status
- RUO
- Workshop
- HCDM listed
- Other Names
- Activation-inducible TNFR, AITR, Glucocorticoid-inducible TNFR, TNFRSF18
- Isotype
- Mouse IgG1, κ
- Ave. Rating
- Submit a Review
- Product Citations
- publications
Cat # | Size | Price | Quantity Check Availability | Save | ||
---|---|---|---|---|---|---|
311611 | 100 µg | 216€ | ||||
311612 | 1 mg | 595€ |
Select size of product is eligible for a 40% discount! Promotion valid until December 31, 2024. Exclusions apply. To view full promotion terms and conditions or to contact your local BioLegend representative to receive a quote, visit our webpage.
GITR (glucocorticoid-induced TNF receptor family-regulated gene) is a 25 kD TNF receptor superfamily member (also known as AITR and TNFRSF18). GITR is expressed on activated lymphocytes and is upregulated by T cell receptor engagement. The cytoplasmic domain of GITR is homologous to CD40, 4-1BB and CD27 and has been shown to interact with TRAF 1-3, but not TRAF 5 or 6. GITR signaling has been shown to regulate T cell proliferation and TCR-mediated apoptosis, and to break immunological self-tolerance. GITR binds GITRL and is involved in the development of regulatory T cells and to regulate the activity of Th1 subsets.
Product DetailsProduct Details
- Verified Reactivity
- Human
- Reported Reactivity
- African Green, Baboon, Cynomolgus, Rhesus
- Antibody Type
- Monoclonal
- Host Species
- Mouse
- Formulation
- 0.2 µm filtered in phosphate-buffered solution, pH 7.2, containing no preservative.
- Endotoxin Level
- Less than 0.01 EU/µg of the protein (< 0.001 ng/µg of the protein) as determined by the LAL test.
- Preparation
- The Ultra-LEAF™ (Low Endotoxin, Azide-Free) antibody was purified by affinity chromatography.
- Concentration
- The antibody is bottled at the concentration indicated on the vial, typically between 2 mg/mL and 3 mg/mL. Older lots may have also been bottled at 1 mg/mL. To obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C. This Ultra-LEAF™ solution contains no preservative; handle under aseptic conditions.
- Application
-
FC - Quality tested
- Recommended Usage
-
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 2.0 µg per million cells in 100 µL volume. It is recommended that the reagent be titrated for optimal performance for each application.
- Application Notes
-
The LEAF™ purified antibody (Endotoxin <0.1 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (Cat. No. 311606).
- Application References
-
- Kwon B, et al. 1999. J. Biol. Chem. 274:6056.
- Zhou Z, et al. 2008. Proc Natl Acad Sci USA . 105:5465 PubMed
- RRID
-
AB_2860827 (BioLegend Cat. No. 311611)
AB_2860827 (BioLegend Cat. No. 311612)
Antigen Details
- Structure
- TNFR superfamily, 234-amino acid type I transmembrane protein, 25 kD
- Distribution
-
Activated lymphocytes
- Function
- Interacts with TRAF's 1-3, but not TRAF 5 or 6, mediates NF-κB activation through the TRAF2/NIK pathway
- Ligand/Receptor
- GITRL
- Cell Type
- Lymphocytes, Tregs
- Biology Area
- Costimulatory Molecules, Immunology
- Molecular Family
- CD Molecules
- Antigen References
-
1. Kwon B, et al. 1999. J. Biol. Chem. 274:6056.
- Gene ID
- 8784 View all products for this Gene ID
- UniProt
- View information about CD357 on UniProt.org
Related FAQs
- I see dim staining with anti human CD357 (GITR) clone 621 in blood and PBMCs.
- Our testing has found that anti human CD357 (GITR) clone 621 stains dim with blood and PBMCs and may only be suitable for situations where GITR levels are high, i.e. stimulated cells or specific cell lines expressing high levels of GITR.
- Do you guarantee that your antibodies are totally pathogen free?
-
BioLegend does not test for pathogens in-house aside from the GoInVivo™ product line. However, upon request, this can be tested on a custom basis with an outside, independent laboratory.
- Does BioLegend test each Ultra-LEAF™ antibody by functional assay?
-
No, BioLegend does not test Ultra-LEAF™ antibodies by functional assays unless otherwise indicated. Due to the possible complexities and variations of uses of biofunctional antibodies in different assays and because of the large product portfolio, BioLegend does not currently perform functional assays as a routine QC for the antibodies. However, we do provide references in which the antibodies were used for functional assays and we do perform QC to verify the specificity and quality of the antibody based on our strict specification criteria.
- Does BioLegend test each Ultra-LEAF™ antibody for potential pathogens?
-
No, BioLegend does not test for pathogens in-house unless otherwise indicated. However, we can recommend an outside vendor to perform this testing as needed.
- Have you tested this Ultra-LEAF™ antibody for in vivo or in vitro applications?
-
We don't test our antibodies for in vivo or in vitro applications unless otherwise indicated. Depending on the product, the TDS may describe literature supporting usage of a particular product for bioassay. It may be best to further consult the literature to find clone specific information.
Other Formats
View All CD357 Reagents Request Custom ConjugationDescription | Clone | Applications |
---|---|---|
PE anti-human CD357 (GITR) | 621 | FC |
Purified anti-human CD357 (GITR) | 621 | FC |
PE/Cyanine5 anti-human CD357 (GITR) | 621 | FC |
APC anti-human CD357 (GITR) | 621 | FC |
Ultra-LEAF™ Purified anti-human CD357 (GITR) | 621 | FC |
Customers Also Purchased
Compare Data Across All Formats
This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.
-
PE anti-human CD357 (GITR)
Human T lymphoma cell line HUT-78 stained with 621 PE -
Purified anti-human CD357 (GITR)
Human T lymphoma cell line HUT-78 stained with purified 621,... -
PE/Cyanine5 anti-human CD357 (GITR)
Human T lymphoma cell line HUT-78 stained with 621 PE/Cyanin... -
APC anti-human CD357 (GITR)
Human T lymphoma cell line HUT-78 stained with 621 APC -
Ultra-LEAF™ Purified anti-human CD357 (GITR)
Human T lymphoma cell line HUT-78 stained with Ultra-LEAF™ p...
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