- Clone
- 3/23 (See other available formats)
- Regulatory Status
- RUO
- Other Names
- Bp50, TNFRSF5
- Isotype
- Rat IgG2a, κ
- Ave. Rating
- Submit a Review
- Product Citations
- publications
Cat # | Size | Price | Quantity Check Availability | Save | ||
---|---|---|---|---|---|---|
124627 | 100 µg | 172€ | ||||
124628 | 1 mg | 498€ |
Select size of product is eligible for a 40% discount! Promotion valid until December 31, 2024. Exclusions apply. To view full promotion terms and conditions or to contact your local BioLegend representative to receive a quote, visit our webpage.
CD40 is a 48 kD type I transmembrane glycoprotein also known as Bp50. It is a member of the tumor necrosis factor receptor (TNFR) superfamily and is expressed on B cells, basal epithelial cells, macrophages, follicular dendritic cells, endothelial cells, and a subset of CD34+ hematopoietic progenitors. CD40 regulates B cell development/maturation, Ig isotype switching and, in combination with other signals such as IL-4, protects B cells from surface Ig-induced apoptosis and promotes proliferation. Interaction of CD40 with its ligand CD154 (gp39), which is expressed on activated T cells, is important in costimulation and immune regulation.
Product DetailsProduct Details
- Verified Reactivity
- Mouse
- Antibody Type
- Monoclonal
- Host Species
- Rat
- Immunogen
- Recombinant mouse CD40 protein
- Formulation
- 0.2 µm filtered in phosphate-buffered solution, pH 7.2, containing no preservative.
- Endotoxin Level
- Less than 0.01 EU/µg of the protein (< 0.001 ng/µg of the protein) as determined by the LAL test.
- Preparation
- The Ultra-LEAF™ (Low Endotoxin, Azide-Free) antibody was purified by affinity chromatography.
- Concentration
- The antibody is bottled at the concentration indicated on the vial, typically between 2 mg/mL and 3 mg/mL. Older lots may have also been bottled at 1 mg/mL. To obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C. This Ultra-LEAF™ solution contains no preservative; handle under aseptic conditions.
- Application
-
FC - Quality tested
- Recommended Usage
-
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 1.0 µg per million cells in 100 µl volume or 100 µl of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.
- Application Notes
-
For highly sensitive assays, we recommend Ultra-LEAF™ purified antibody (Cat. No. 124627 &124628) with a low endotoxin limit (Endotoxin < 0.01 EU/µg).
- Application References
-
- Hasbold J, et al. 1994. Eur. J. Immunol. 24:1835.
- Bourgeois C, et al. 2002. Science 297:2060.
- RRID
-
AB_2561490 (BioLegend Cat. No. 124627)
AB_2561490 (BioLegend Cat. No. 124628)
Antigen Details
- Structure
- 48 kD type I transmembrane glycoprotein, a member of the tumor necrosis factor receptor (TNFR) superfamily.
- Distribution
-
Expressed on B lymphocytes and other antigen-presenting cells, such as macrophages, follicular dendritic cells, etc.
- Function
- Regulates B cell growth and differentiation and Ig isotype switching. Interaction of CD40 with its ligand, CD154, on activated T cells is important for immune response.
- Ligand/Receptor
- CD154 on T cells
- Cell Type
- Antigen-presenting cells, B cells, Dendritic cells, Macrophages
- Biology Area
- Cell Biology, Costimulatory Molecules, Immunology, Neuroscience, Neuroscience Cell Markers
- Molecular Family
- CD Molecules
- Antigen References
-
1. Grewal IS, et al. 1998. Annu Rev Immunol 16:111.
- Gene ID
- 21939 View all products for this Gene ID
- UniProt
- View information about CD40 on UniProt.org
Related FAQs
- Do you guarantee that your antibodies are totally pathogen free?
-
BioLegend does not test for pathogens in-house aside from the GoInVivo™ product line. However, upon request, this can be tested on a custom basis with an outside, independent laboratory.
- Does BioLegend test each Ultra-LEAF™ antibody by functional assay?
-
No, BioLegend does not test Ultra-LEAF™ antibodies by functional assays unless otherwise indicated. Due to the possible complexities and variations of uses of biofunctional antibodies in different assays and because of the large product portfolio, BioLegend does not currently perform functional assays as a routine QC for the antibodies. However, we do provide references in which the antibodies were used for functional assays and we do perform QC to verify the specificity and quality of the antibody based on our strict specification criteria.
- Does BioLegend test each Ultra-LEAF™ antibody for potential pathogens?
-
No, BioLegend does not test for pathogens in-house unless otherwise indicated. However, we can recommend an outside vendor to perform this testing as needed.
- Have you tested this Ultra-LEAF™ antibody for in vivo or in vitro applications?
-
We don't test our antibodies for in vivo or in vitro applications unless otherwise indicated. Depending on the product, the TDS may describe literature supporting usage of a particular product for bioassay. It may be best to further consult the literature to find clone specific information.
Other Formats
View All CD40 Reagents Request Custom ConjugationDescription | Clone | Applications |
---|---|---|
Purified anti-mouse CD40 | 3/23 | FC,IHC-F |
Biotin anti-mouse CD40 | 3/23 | FC |
FITC anti-mouse CD40 | 3/23 | FC |
PE anti-mouse CD40 | 3/23 | FC |
APC anti-mouse CD40 | 3/23 | FC |
Alexa Fluor® 647 anti-mouse CD40 | 3/23 | FC |
PE/Cyanine5 anti-mouse CD40 | 3/23 | FC |
PE/Cyanine7 anti-mouse CD40 | 3/23 | FC |
Ultra-LEAF™ Purified anti-mouse CD40 | 3/23 | FC |
PerCP/Cyanine5.5 anti-mouse CD40 | 3/23 | FC |
Pacific Blue™ anti-mouse CD40 | 3/23 | FC |
PE/Dazzle™ 594 anti-mouse CD40 | 3/23 | FC |
APC/Fire™ 750 anti-mouse CD40 | 3/23 | FC |
TotalSeq™-C0903 anti-mouse CD40 | 3/23 | PG |
TotalSeq™-A0903 anti-mouse CD40 | 3/23 | PG |
APC/Cyanine7 anti-mouse CD40 | 3/23 | FC |
TotalSeq™-B0903 anti-mouse CD40 Antibody | 3/23 | PG |
Brilliant Violet 421™ anti-mouse CD40 | 3/23 | FC |
Brilliant Violet 650™ anti-mouse CD40 | 3/23 | FC |
Brilliant Violet 785™ anti-mouse CD40 | 3/23 | FC |
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Compare Data Across All Formats
This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.
-
Purified anti-mouse CD40
BALB/c mouse splenocytes stained with purified 3/23, followe... Balb/C mouse frozen spleen tissue slices were fixed with 4% ... -
Biotin anti-mouse CD40
BALB/c splenocytes stained with biotinylated 3/23, followed ... -
FITC anti-mouse CD40
BALB/c splenocytes stained with 3/23 FITC -
PE anti-mouse CD40
BALB/c splenocytes stained with 3/23 PE -
APC anti-mouse CD40
BALB/c splenocytes stained with 3/23 APC -
Alexa Fluor® 647 anti-mouse CD40
BALB/c splenocytes stained with 3/23 Alexa Fluor® 647 -
PE/Cyanine5 anti-mouse CD40
C57BL/6 splenocytes stained with 3/23 PE/Cyanine5 -
PE/Cyanine7 anti-mouse CD40
BALB/c splenocytes stained with CD40 (clone 3/23) PE/Cyanine... -
Ultra-LEAF™ Purified anti-mouse CD40
BALB/c mouse splenocytes stained with Ultra-LEAF™ purified C... -
PerCP/Cyanine5.5 anti-mouse CD40
C57BL/6 mouse splenocytes were stained with CD40 (clone 3/23... -
Pacific Blue™ anti-mouse CD40
C57BL/6 mouse splenocytes were stained with CD40 (clone 3/23... -
PE/Dazzle™ 594 anti-mouse CD40
C57BL/6 mouse splenocytes were stained with CD40 (clone 3/23... -
APC/Fire™ 750 anti-mouse CD40
C57BL/6 mouse splenocytes were stained with CD40 (clone 3/23... -
TotalSeq™-C0903 anti-mouse CD40
-
TotalSeq™-A0903 anti-mouse CD40
-
APC/Cyanine7 anti-mouse CD40
C57BL/6 mouse splenocytes were stained with CD40 (clone 3/23... -
TotalSeq™-B0903 anti-mouse CD40 Antibody
-
Brilliant Violet 421™ anti-mouse CD40
C57BL/6 mouse splenocytes were stained with anti-mouse CD19 ... -
Brilliant Violet 650™ anti-mouse CD40
C57BL/6 mouse splenocytes were stained with anti-mouse CD19 ... -
Brilliant Violet 785™ anti-mouse CD40
C57BL/6 mouse splenocytes were stained with anti-mouse CD19 ...
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