Ultra-LEAF™ Purified anti-mouse IFN-γ Antibody

Product Details

Verified Reactivity

Mouse

Antibody Type

Monoclonal

Host Species

Armenian Hamster

Immunogen

Recombinant full-length murine IFN-γ

Formulation

0.2 µm filtered in phosphate-buffered solution, pH 7.2, containing no preservative.

Endotoxin Level

Less than 0.01 EU/µg of the protein (< 0.001 ng/µg of the protein) as determined by the LAL test.

Preparation

The Ultra-LEAF™ (Low Endotoxin, Azide-Free) antibody was purified by affinity chromatography.

Concentration

The antibody is bottled at the concentration indicated on the vial, typically between 2 mg/mL and 3 mg/mL. Older lots may have also been bottled at 1 mg/mL. To obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.

Storage & Handling

The antibody solution should be stored undiluted between 2°C and 8°C. This Ultra-LEAF™ solution contains no preservative; handle under aseptic conditions.

Application

WB - Quality tested
Neut, ELISA, ICC, IP - Reported in the literature, not verified in house

Recommended Usage

Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 0.5 µg per mL. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

The purified H22 antibody is useful in mouse IFN-γ ELISA, immunofluorescence and immunoprecipitation applications. For in vitro or in vivo neutralization applications, it is suggested to use LEAF™ Purified H22. It is recommended that the reagent be titrated for optimal performance for each application.

Application References

1. Schreiber RD, et al. 1985. J. Immunol. 134:1609.
2. Williams RO, et al. 1993. Clin. Exp. Immunol. 92:323
3. Steed AL, et al. 2006. J. Virol. 80:192.
4. Wu LL, et al. 2014. Am J Pathol. 184:2260. PubMed

Product Citations

1. Do-Thi VA, et al. 2023. Cancer Res Commun. 3:80. PubMed

RRID

AB_2810645 (BioLegend Cat. No. 513212)
AB_2810645 (BioLegend Cat. No. 513210)
AB_2810645 (BioLegend Cat. No. 513211)
AB_2810645 (BioLegend Cat. No. 513207)
AB_2810645 (BioLegend Cat. No. 513208)
AB_2810645 (BioLegend Cat. No. 513209)

Antigen Details

Structure

Cytokine; dimer; 40-80 kD (Mammalian)

Function

Upregulated by IL-2, FGF-basic, EGF; downregulated by 1-α-25-Dihydroxy vitamin D3, dexamethasone

Interaction

T cells, B cells, macrophages, NK cells, endothelial cells, fibroblasts

Ligand/Receptor

IFN-γRα (CDw119) dimerized with IFN-γRβ (AF-1)

Bioactivity

Antiviral/antiparasitic activities; inhibits proliferation; enhances MHC class I and II expression on APC

Cell Sources

CD8⁺ and CD4⁺ T cells, NK cells

Biology Area

Immunology

Molecular Family

Cytokines/Chemokines

Antigen References

1. Fitzgerald K, et al. Eds. 2001. The Cytokine FactsBook. Academic Press San Diego.
2. De Maeyer E, et al. 1992. Curr. Opin. Immunol. 4:321
3. Farrar M, et al. 1993. Annu. Rev. Immunol. 11:571
4. Gray P, et al. 1987. Lymphokines 13:151

Gene ID

15978 View all products for this Gene ID

UniProt

View information about IFN-gamma on UniProt.org

Documentation

• Technical Data Sheet (pdf)
• Certificate of Analysis
• Material Safety Data Sheet

Reviews

Related Protocols

• Western Blotting Protocol

Related Pages & Pathways

Related FAQs

Do you guarantee that your antibodies are totally pathogen free?

BioLegend does not test for pathogens in-house aside from the GoInVivo™ product line. However, upon request, this can be tested on a custom basis with an outside, independent laboratory.

Does BioLegend test each Ultra-LEAF™ antibody by functional assay?

No, BioLegend does not test Ultra-LEAF™ antibodies by functional assays unless otherwise indicated. Due to the possible complexities and variations of uses of biofunctional antibodies in different assays and because of the large product portfolio, BioLegend does not currently perform functional assays as a routine QC for the antibodies. However, we do provide references in which the antibodies were used for functional assays and we do perform QC to verify the specificity and quality of the antibody based on our strict specification criteria.

Does BioLegend test each Ultra-LEAF™ antibody for potential pathogens?

No, BioLegend does not test for pathogens in-house unless otherwise indicated.  However, we can recommend an outside vendor to perform this testing as needed.

Have you tested this Ultra-LEAF™ antibody for in vivo or in vitro applications?

We don't test our antibodies for in vivo or in vitro applications unless otherwise indicated. Depending on the product, the TDS may describe literature supporting usage of a particular product for bioassay. It may be best to further consult the literature to find clone specific information.

Other Formats

Certificate of Analysis