- Clone
- TC11-18H10.1 (See other available formats)
- Regulatory Status
- RUO
- Other Names
- Interleukin-17, Cytotoxic T lymphocyte-associated antigen 8 (CTLA-8)
- Isotype
- Rat IgG1, κ
- Ave. Rating
- Submit a Review
- Product Citations
- publications
Cat # | Size | Price | Quantity Check Availability | Save | ||
---|---|---|---|---|---|---|
506945 | 100 µg | 146€ | ||||
506946 | 1 mg | 480€ |
Select size of product is eligible for a 40% discount! Promotion valid until December 31, 2024. Exclusions apply. To view full promotion terms and conditions or to contact your local BioLegend representative to receive a quote, visit our webpage.
IL-17, also known as CTLA-8, is a T cell-expressed pleiotropic cytokine that exhibits a high degree of homology to a protein encoded by the ORF13 gene of herpes virus Saimiri. IL-17 is produced by Th cells (Th17) that are distinct from the traditional Th1- and Th2-cell subsets. IL-23 plays an important role in triggering IL-17 production. Both recombinant and natural IL-17 have been shown to exist as disulfide linked homodimers. IL-17 exhibits multiple biological activities on a variety of cells including: the induction of IL-6 and IL-8 production in fibroblasts, activation of NF-κB, and costimulation of T cell proliferation. IL-17 is an essential inflammatory mediator in the development of autoimmune diseases. Neutralization of IL-17 with monoclonal antibody is able to ameliorate the disease course.
Product DetailsProduct Details
- Verified Reactivity
- Mouse
- Antibody Type
- Monoclonal
- Host Species
- Rat
- Immunogen
- E. coli expressed, recombinant mouse IL-17A
- Formulation
- 0.2 µm filtered in phosphate-buffered solution, pH 7.2, containing no preservative.
- Endotoxin Level
- Less than 0.01 EU/µg of the protein (< 0.001 ng/µg of the protein) as determined by the LAL test.
- Preparation
- The Ultra-LEAF™ (Low Endotoxin, Azide-Free) antibody was purified by affinity chromatography.
- Concentration
- The antibody is bottled at the concentration indicated on the vial, typically between 2 mg/mL and 3 mg/mL. Older lots may have also been bottled at 1 mg/mL. To obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C. This Ultra-LEAF™ solution contains no preservative; handle under aseptic conditions.
- Application
-
ELISA Capture - Quality tested
CyTOF® - Verified
ELISPOT Capture, ICFC - Reported in the literature, not verified in house - Recommended Usage
-
Each lot of this antibody is quality control tested by ELISA assay. For ELISA capture, a concentration range of 2-6 µg/ml is recommended. To obtain a linear standard curve, serial dilutions of IL-17 recombinant protein ranging from 4000 to 30 pg/ml are recommended for each ELISA plate. It is recommended that the reagent be titrated for optimal performance for each application.
- Application Notes
-
ELISA Capture3,4 and ELISPOT Capture5: The purified TC11-18H10.1 antibody is useful as the capture antibody in a sandwich ELISA, when used in conjunction with the biotinylated TC11-8H4 antibody (Cat. No. 507002) as the detecting antibody and recombinant mouse IL-17 (Cat. No. 576009) as the standard.
Flow Cytometry2-4,7,8,11,12: The TC11-18H10.1 antibody is useful for intracellular immunofluorescent staining and flow cytometric analysis to identify IL-17-producing cells within mixed cell populations.
Neutralization6,9: The LEAF™ purified antibody (Endotoxin <0.1 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for neutralization of mouse IL-17 bioactivity in vivo and in vitro (Cat. No. 506906). - Application References
-
- Kennedy J, et al. 1996. J. Interferon Cytokine Res. 16:611.
- Schubert D, et al. 2004. J. Immunol. 172:4503. (ICFC)
- Infante-Duarte C, et al. 2000. J. Immunol. 165:6107. (ICFC, ELISA Capture)
- Harrington LE, et al. 2005. Nature Immunol. doi:10.1038/ni1254. (ICFC, ELISA Capture)
- Nekrasova T, et al. 2005. J. Immunol. 175:2734. (ELISPOT Capture)
- Yen D, et al. 2006. J. Clin. Invest. 116:1310. (Neut)
- Ehirchiou D, et al. 2007. J. Exp. Med. 204:1519. (ICFC)
- Kang SG, et al. 2007. J. Immunol. 179:3724. (ICFC)
- Smith E, et al. 2008. J. Immunol. 181:1357. (Neut) PubMed
- Neufert C, et al. 2007. Eur. J. Immunol. 37:1809. PubMed
- Wang C, et al. 2009. Mucosal Immunol 2:173. (ICFC) PubMed
- Cui Y, et al. 2009. Invest. Ophth. Vis. Sci. 50:5811. (ICFC) PubMed
- Kivisäkk P, et al. 2009. Ann. Neurol. 65:457. PubMed
- Cooney LA, et al. 2011. J. Immunol. 187:4440. PubMed
- Ma Y, et al. 2012. PLoS One. 7:e40763. PubMed
- Murakami R, et al. 2013. PLoS One. 8:73270. PubMed
- Product Citations
-
- RRID
-
AB_2783334 (BioLegend Cat. No. 506945)
AB_2783334 (BioLegend Cat. No. 506946)
Antigen Details
- Structure
- Cytokine; dimer; 15 kD (Mammalian).
- Bioactivity
- Secretion of IL-6, IL-8, G-CSF, prostaglandin E2 by epithelial, endothelial or fibroblastic cells; stimulates cell migration, cord formation, and IL-6 secretion by stromal cells
- Cell Sources
- CD4+ memory T cells
- Cell Targets
- Fibroblasts, epithelial and endothelial cells, stromal cells
- Receptors
- IL-17R (CD217)
- Biology Area
- Cell Biology, Immunology, Neuroinflammation, Neuroscience
- Molecular Family
- Cytokines/Chemokines
- Antigen References
-
1. Fitzgerald K, et al. Eds. 2001. The Cytokine FactsBook. Academic Press San Diego.
2. Numasaki M, et al. 2002. Blood 101:2620.
3. Fossiez F, et al. 1996. J. Exp. Med. 183:2593.
4. Yao Z, et al. 1997. Cytokine 9:794.
5. Dong C. 2006. Nat. Rev. Immunol. 6:329.
6. Hofstetter HH, et al. 2005 Cell. Immunol. 237:123. - Gene ID
- 16171 View all products for this Gene ID
- UniProt
- View information about IL-17A on UniProt.org
Related FAQs
- Does the anti-mouse IL-17 antibody (clone TC11-18H10.1) recognize the A or F isoform?
- Clone TC11-18H10.1 recognizes IL-17A isoform, but it also recognizes the IL-17A/F heterodimer via IL-17A binding.
- Do you guarantee that your antibodies are totally pathogen free?
-
BioLegend does not test for pathogens in-house aside from the GoInVivo™ product line. However, upon request, this can be tested on a custom basis with an outside, independent laboratory.
- Does BioLegend test each Ultra-LEAF™ antibody by functional assay?
-
No, BioLegend does not test Ultra-LEAF™ antibodies by functional assays unless otherwise indicated. Due to the possible complexities and variations of uses of biofunctional antibodies in different assays and because of the large product portfolio, BioLegend does not currently perform functional assays as a routine QC for the antibodies. However, we do provide references in which the antibodies were used for functional assays and we do perform QC to verify the specificity and quality of the antibody based on our strict specification criteria.
- Does BioLegend test each Ultra-LEAF™ antibody for potential pathogens?
-
No, BioLegend does not test for pathogens in-house unless otherwise indicated. However, we can recommend an outside vendor to perform this testing as needed.
- Have you tested this Ultra-LEAF™ antibody for in vivo or in vitro applications?
-
We don't test our antibodies for in vivo or in vitro applications unless otherwise indicated. Depending on the product, the TDS may describe literature supporting usage of a particular product for bioassay. It may be best to further consult the literature to find clone specific information.
Other Formats
View All IL-17A Reagents Request Custom ConjugationCustomers Also Purchased
Compare Data Across All Formats
This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.
-
PE anti-mouse IL-17A
PMA (20 ng/ml) + ionomycin (1 µg/ml) -stimulated (6 ho... PMA/ionomycin-stimulated (5 hours) Th17 polarized C57BL/6 mo... -
Purified anti-mouse IL-17A
-
FITC anti-mouse IL-17A
PMA (20 ng/ml) + ionomycin (1 µg/ml) -stimulated (6 ho... -
Alexa Fluor® 488 anti-mouse IL-17A
PMA (20 ng/ml) + ionomycin (1 µg/ml) -stimulated (6 ho... -
Alexa Fluor® 647 anti-mouse IL-17A
PMA (20 ng/ml) + ionomycin (1 µg/ml) -stimulated (6 ho... PMA/ionomycin-stimulated (5 hours) Th17 polarized C57BL/6 mo... -
Alexa Fluor® 700 anti-mouse IL-17A
6 hours PMA (20 ng/ml) + ionomycin (1 µg/ml) -stimulat... -
APC anti-mouse IL-17A
PMA (20 ng/ml) + ionomycin (1 µg/ml) -stimulated (6 ho... -
Pacific Blue™ anti-mouse IL-17A
PMA (20 ng/ml) + ionomycin (1 µg/ml) -stimulated (6 ho... -
PerCP/Cyanine5.5 anti-mouse IL-17A
PMA + ionomycin-stimulated (6 hours) mouse lymphoma cell lin... -
PE/Cyanine7 anti-mouse IL-17A
PMA (20 ng/ml) + ionomycin (1 µg/ml) -stimulated (6 ho... -
Brilliant Violet 421™ anti-mouse IL-17A
Th17-polarized C57BL/6 mouse CD4+ lymphocytes were stimulate... -
Brilliant Violet 605™ anti-mouse IL-17A
Th17-polarized mouse CD4+ lymphocytes were stimul... -
Brilliant Violet 650™ anti-mouse IL-17A
Th17-polarized C57BL/6 mouse CD4+ lymphocytes were stimulate... -
Brilliant Violet 785™ anti-mouse IL-17A
PMA + ionomycin-stimulated (six hours) mouse thymoma cell li... -
Brilliant Violet 510™ anti-mouse IL-17A
PMA + ionomycin-stimulated (6 hours) mouse thymoma cell lin... -
Purified anti-mouse IL-17A (Maxpar® Ready)
Mouse EL4 T cells were incubated for 5 hours in media alone ... -
PE/Dazzle™ 594 anti-mouse IL-17A
PMA + ionomycin-stimulated (6 hours) mouse thymoma cell line... -
APC/Cyanine7 anti-mouse IL-17A
PMA + ionomycin-stimulated (six hours) mouse thymoma cell li... -
Brilliant Violet 711™ anti-mouse IL-17A
Th17-polarized mouse CD4+ lymphocytes were stimulated with P... -
PerCP anti-mouse IL-17A
PMA + ionomycin-stimulated in the presence of monensin (6 ho... -
Ultra-LEAF™ Purified anti-mouse IL-17A
-
PE/Cyanine5 anti-mouse IL-17A
Th17-polarized C57BL/6 mouse CD4+ lymphocytes were stimulate... PMA+ ionomycin-stimulated (6 hours) mouse thymoma cell line ...
Follow Us