- Clone
- MECA-367 (See other available formats)
- Regulatory Status
- RUO
- Other Names
- Mucosal addressin cell adhesion molecule-1
- Isotype
- Rat IgG2a, κ
- Ave. Rating
- Submit a Review
- Product Citations
- publications
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MAdCAM-1 is a 58-66kD type I glycoprotein, also known as Mucosal addressin cell adhesion molecule-1. This mucosal vascular addressin is a member of the Ig superfamily found on fetus and neonatal endothelial cells. In adults, MAdCAM-1 is predominately expressed on high endothelial venule (HEV) of Peyer's patches, mesenteric lymph nodes and gut lamina propria. It is also expressed on vascular endothelium in mammary glands and pancreas. MAdCAM-1, through its interaction with integrin α4β7 or CD62L, is involved in lymphocyte tethering, rolling, and homing. It has been reported that immobilized MAdCAM-1 is able to co-stimulate T cell proliferation. The MECA-367 antibody blocks the interaction of MAdCAM-1 with its counter-receptor both in vitro and in vivo. In vivo administration of the mAb is able to reduce T-cell mediated inflammation in some gastrointestinal diseases.
Product DetailsProduct Details
- Verified Reactivity
- Mouse
- Antibody Type
- Monoclonal
- Host Species
- Rat
- Immunogen
- Endothelial cells
- Formulation
- 0.2 µm filtered in phosphate-buffered solution, pH 7.2, containing no preservative.
- Endotoxin Level
- Less than 0.01 EU/µg of the protein (< 0.001 ng/µg of the protein) as determined by the LAL test.
- Concentration
- The antibody is bottled at the concentration indicated on the vial, typically between 2 mg/mL and 3 mg/mL. Older lots may have also been bottled at 1 mg/mL. To obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C. This Ultra-LEAF™ solution contains no preservative; handle under aseptic conditions.
- Application
-
FC - Quality tested
- Recommended Usage
-
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 µg per million cells in 100 µL volume. It is recommended that the reagent be titrated for optimal performance for each application.
- Application Notes
-
Additional reported applications (for the relevant formats) include: in vitro and in vivo blocking of lymphocyte adhesion and in vivo blocking of lymphocyte homing1-4,7, immunohistochemical staining1,5,6 of acetone-fixed frozen sections, immunoprecipitation, and Western blotting1.
- Application References
-
- Streeter PR, et al. 1988. Nature 331:41.
- Briskin MJ, et al. 1993. Nature 363:461.
- Berlin C, et al. 1993. Cell 74:185.
- Bargatze RF, et al. 1995. Immunity 3:99.
- Tanneau GM, et al. 1999. J. Histochem. Cytochem. 47:1581.
- Savinov AY, et al. 2003. J. Exp. Med. 197:643.
- Rivera-Nieves J, et al. 2005. J. Immunol. 174:2343.
- Hindley JP, et al. 2012. Cancer Res. 72:5473. PubMed.
- RRID
-
AB_2832426 (BioLegend Cat. No. 120720)
AB_2832426 (BioLegend Cat. No. 120718)
AB_2832426 (BioLegend Cat. No. 120719)
AB_2832426 (BioLegend Cat. No. 120715)
AB_2832426 (BioLegend Cat. No. 120716)
AB_2832426 (BioLegend Cat. No. 120717)
Antigen Details
- Structure
- Type I glycoprotein, Ig superfamily
- Distribution
-
HEV of mucosal lymphoid organ and lamina propia, endothelial cells in mammary glands and pancreas
- Function
- Lymphocyte tethering, rolling and homing, costimulate T cell proliferatioon
- Ligand/Receptor
- Integrin a4ß7, CD62L
- Cell Type
- Endothelial cells
- Biology Area
- Cell Adhesion, Cell Biology, Immunology
- Molecular Family
- Adhesion Molecules
- Antigen References
-
1. Streeter PR, et al. 1988. Nature 331:41
2. Briskin MJ, et al. 1993.Nature 363:461.
3. Berlin C, et al. 1993. Cell 74:185.
4. Lehnert K, et al. 1998. Eur. J. Immunol. 28:3605.
5. Picarella D, et al. 1997. J. Immunol. 158:2099. - Gene ID
- 17123 View all products for this Gene ID
- UniProt
- View information about MAdCAM-1 on UniProt.org
Related Pages & Pathways
Pages
Related FAQs
- Do you guarantee that your antibodies are totally pathogen free?
-
BioLegend does not test for pathogens in-house aside from the GoInVivo™ product line. However, upon request, this can be tested on a custom basis with an outside, independent laboratory.
- Does BioLegend test each Ultra-LEAF™ antibody by functional assay?
-
No, BioLegend does not test Ultra-LEAF™ antibodies by functional assays unless otherwise indicated. Due to the possible complexities and variations of uses of biofunctional antibodies in different assays and because of the large product portfolio, BioLegend does not currently perform functional assays as a routine QC for the antibodies. However, we do provide references in which the antibodies were used for functional assays and we do perform QC to verify the specificity and quality of the antibody based on our strict specification criteria.
- Does BioLegend test each Ultra-LEAF™ antibody for potential pathogens?
-
No, BioLegend does not test for pathogens in-house unless otherwise indicated. However, we can recommend an outside vendor to perform this testing as needed.
- Have you tested this Ultra-LEAF™ antibody for in vivo or in vitro applications?
-
We don't test our antibodies for in vivo or in vitro applications unless otherwise indicated. Depending on the product, the TDS may describe literature supporting usage of a particular product for bioassay. It may be best to further consult the literature to find clone specific information.
Other Formats
View All MAdCAM-1 Reagents Request Custom ConjugationDescription | Clone | Applications |
---|---|---|
Purified anti-mouse MAdCAM-1 | MECA-367 | FC,IHC-F,IP,WB |
Biotin anti-mouse MAdCAM-1 | MECA-367 | FC |
Alexa Fluor® 488 anti-mouse MAdCAM-1 | MECA-367 | FC |
PE anti-mouse MAdCAM-1 | MECA-367 | FC |
APC anti-mouse MAdCAM-1 | MECA-367 | FC |
Ultra-LEAF™ Purified anti-mouse MAdCAM-1 | MECA-367 | FC |
TotalSeq™-C0232 anti-mouse MAdCAM-1 | MECA-367 | PG |
TotalSeq™-B0232 anti-mouse MAdCAM-1 | MECA-367 | PG |
Compare Data Across All Formats
This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.
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