- Clone
- MOPC-173 (See other available formats)
- Regulatory Status
- RUO
- Isotype
- Mouse IgG2a, κ
- Ave. Rating
- Submit a Review
- Product Citations
- publications
Select size of product is eligible for a 40% discount! Promotion valid until September 30, 2024. Exclusions apply. To view full promotion terms and conditions or to contact your local BioLegend representative to receive a quote, visit our webpage.
The MOPC-173 immunoglobulin has unknown specificity. The isotype of this antibody is mouse IgG2a, κ. This antibody was chosen as an isotype control after screening on a variety of resting, activated, live, and fixed mouse, rat, and human tissues.
Product DetailsProduct Details
- Antibody Type
- Monoclonal
- Host Species
- Mouse
- Formulation
- 0.2 µm filtered in phosphate-buffered solution, pH 7.2, containing no preservative.
- Endotoxin Level
- Less than 0.01 EU/µg of the protein (< 0.001 ng/µg of the protein) as determined by the LAL test.
- Preparation
- The Ultra-LEAF™ (Low Endotoxin, Azide-Free) antibody was purified by affinity chromatography.
- Concentration
- The antibody is bottled at the concentration indicated on the vial, typically between 2 mg/mL and 3 mg/mL. Older lots may have also been bottled at 1 mg/mL. To obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C. This Ultra-LEAF™ solution contains no preservative; handle under aseptic conditions.
- Application
-
FC - Quality tested
ICFC, ICC, IHC, IP, WB, FA - Reported in the literature, not verified in house - Recommended Usage
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Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis as negative control. Use at concentrations comparable to those of the specific antibody of interest.
- Application Notes
-
Additional reported applications (for the relevant formats) include: Intracellular Flow Cytometry (ICFC), Immunocytochemistry (ICC), Immunohistochemistry (IHC), Immunoprecipitation (IP), Western Blotting (WB), Functional Assay (FA).
- Product Citations
-
- RRID
-
AB_11148947 (BioLegend Cat. No. 400282)
AB_11148947 (BioLegend Cat. No. 400289)
AB_11148947 (BioLegend Cat. No. 400290)
AB_11149166 (BioLegend Cat. No. 400263)
AB_11148947 (BioLegend Cat. No. 400264)
AB_11148947 (BioLegend Cat. No. 400281)
Antigen Details
- Gene ID
- NA
Related Pages & Pathways
Pages
Related FAQs
- IgG2a gene is deleted in some mouse strains, which ones are they?
-
IgG2a gene is deleted in C57Bl/6, C57Bl/10, SJL, and NOD mice. It is replaced with IgG2c gene instead.
- Do you guarantee that your antibodies are totally pathogen free?
-
BioLegend does not test for pathogens in-house aside from the GoInVivo™ product line. However, upon request, this can be tested on a custom basis with an outside, independent laboratory.
- Does BioLegend test each Ultra-LEAF™ antibody by functional assay?
-
No, BioLegend does not test Ultra-LEAF™ antibodies by functional assays unless otherwise indicated. Due to the possible complexities and variations of uses of biofunctional antibodies in different assays and because of the large product portfolio, BioLegend does not currently perform functional assays as a routine QC for the antibodies. However, we do provide references in which the antibodies were used for functional assays and we do perform QC to verify the specificity and quality of the antibody based on our strict specification criteria.
- Does BioLegend test each Ultra-LEAF™ antibody for potential pathogens?
-
No, BioLegend does not test for pathogens in-house unless otherwise indicated. However, we can recommend an outside vendor to perform this testing as needed.
- Have you tested this Ultra-LEAF™ antibody for in vivo or in vitro applications?
-
We don't test our antibodies for in vivo or in vitro applications unless otherwise indicated. Depending on the product, the TDS may describe literature supporting usage of a particular product for bioassay. It may be best to further consult the literature to find clone specific information.
Other Formats
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Compare Data Across All Formats
This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.
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APC Mouse IgG2a, κ Isotype Ctrl
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Biotin Mouse IgG2a, κ Isotype Ctrl
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FITC Mouse IgG2a, κ Isotype Ctrl
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PE Mouse IgG2a, κ Isotype Ctrl
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PE/Cyanine5 Mouse IgG2a, κ Isotype Ctrl
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Purified Mouse IgG2a, κ Isotype Ctrl
Chromatin Immunoprecipitations (ChIP) were performed with cr... -
APC/Cyanine7 Mouse IgG2a, κ Isotype Ctrl
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PE/Cyanine7 Mouse IgG2a, κ Isotype Ctrl
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Alexa Fluor® 647 Mouse IgG2a, κ Isotype Ctrl
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Alexa Fluor® 488 Mouse IgG2a, κ Isotype Ctrl
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Pacific Blue™ Mouse IgG2a, κ Isotype Ctrl
-
PE Mouse IgG2a, κ Isotype Ctrl (ICFC)
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FITC Mouse IgG2a, κ Isotype Ctrl (FC)
-
PE Mouse IgG2a, κ Isotype Ctrl (FC)
-
APC Mouse IgG2a, κ Isotype Ctrl (FC)
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Brilliant Violet 605™ Mouse IgG2a, κ Isotype Ctrl
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Alexa Fluor® 700 Mouse IgG2a, κ Isotype Ctrl
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PerCP Mouse IgG2a, κ Isotype Ctrl
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PerCP/Cyanine5.5 Mouse IgG2a, κ Isotype Ctrl
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Brilliant Violet 421™ Mouse IgG2a, κ Isotype Ctrl
-
Brilliant Violet 570™ Mouse IgG2a, κ Isotype Ctrl
-
Ultra-LEAF™ Purified Mouse IgG2a, κ Isotype Ctrl
-
Brilliant Violet 510™ Mouse IgG2a, κ Isotype Ctrl
-
Brilliant Violet 650™ Mouse IgG2a, κ Isotype Ctrl
-
Brilliant Violet 711™ Mouse IgG2a, κ Isotype Ctrl
-
Brilliant Violet 785™ Mouse IgG2a, κ Isotype Ctrl
-
PE/Dazzle™ 594 Mouse IgG2a, κ Isotype Ctrl
-
Alexa Fluor® 594 Mouse IgG2a, κ Isotype Ctrl
-
FITC Mouse IgG2a, κ Isotype Ctrl
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APC/Fire™ 750 Mouse IgG2a, κ Isotype Ctrl
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Pacific Blue™ Mouse IgG2a, κ Isotype Ctrl
-
APC Mouse IgG2a, κ Isotype Ctrl
-
PE/Dazzle™ 594 Mouse IgG2a, κ Isotype Ctrl
-
PE Mouse IgG2a, κ Isotype Ctrl
-
TotalSeq™-A0091 Mouse IgG2a, κ isotype Ctrl
-
TotalSeq™-B0091 Mouse IgG2a, κ isotype Ctrl
-
TotalSeq™-C0091 Mouse IgG2a, κ isotype Ctrl
-
PE/Cyanine7 Mouse IgG2a, κ Isotype Ctrl
-
APC/Fire™ 750 Mouse IgG2a, κ Isotype Ctrl
-
PerCP/Cyanine5.5 Mouse IgG2a, κ Isotype Ctrl
-
KIRAVIA Blue 520™ Mouse IgG2a, κ Isotype Ctrl
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Spark Blue™ 550 Mouse IgG2a, κ Isotype Ctrl
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Spark NIR™ 685 Mouse IgG2a, κ Isotype Ctrl
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Spark Violet™ 538 Mouse IgG2a, κ Isotype Ctrl
-
Spark YG™ 581 Mouse IgG2a, κ Isotype Ctrl
-
TotalSeq™-D0091 Mouse IgG2a, κ Isotype Ctrl
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Spark YG™ 593 Mouse IgG2a, κ Isotype Ctrl
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GMP PE Mouse IgG2a, κ Isotype Ctrl
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GMP FITC Mouse IgG2a, κ Isotype Ctrl
-
GMP APC Mouse IgG2a, κ Isotype Ctrl
-
GMP PE/Dazzle™ 594 Mouse IgG2a, κ Isotype Ctrl
-
GMP APC/Fire™ 750 Mouse IgG2a, κ Isotype Ctrl
-
GMP PerCP/Cyanine5.5 Mouse IgG2a, κ Isotype Ctrl
-
PE/Fire™ 700 Mouse IgG2a, κ Isotype Ctrl
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PE/Fire™ 810 Mouse IgG2a, κ Isotype Ctrl
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PE/Fire™ 640 Mouse IgG2a, κ Isotype Ctrl
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GMP PE/Cyanine7 Mouse IgG2a, κ Isotype Ctrl
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GMP Pacific Blue™ Mouse IgG2a, κ Isotype Ctrl
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Spark Violet™ 423 Mouse IgG2a, κ Isotype Ctrl
-
APC/Fire™ 810 Mouse IgG2a, κ Isotype Ctrl
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PerCP/Fire™ 806 Mouse IgG2a, κ Isotype Ctrl
-
PerCP/Fire™ 780 Mouse IgG2a, κ Isotype Ctrl
-
Spark Red™ 718 Mouse IgG2a, κ Isotype Ctrl
-
Spark Blue™ 515 Mouse IgG2a, κ Isotype Ctrl
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Spark Blue™ 574 Mouse IgG2a, κ Isotype Ctrl
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PE/Fire™ 744 Mouse IgG2a, κ Isotype Ctrl
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Alexa Fluor® 660 Mouse IgG2a, κ Isotype Ctrl
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Spark UV™ 387 Mouse IgG2a, κ Isotype Ctrl
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