Anti-Alpha Smooth Muscle Actin Antibody anti-Alpha Smooth Muscle Actin

Pricing & Availability

Clone: 1A4 (See other available formats)
Regulatory Status: RUO
Workshop: HCDM listed
Other Names: Actin, aortic smooth muscle, alpha 2 actin, alpha-actin-2, alpha-cardiac actin, growth-inhibiting gene 46, cell growth-inhibiting gene 46 protein
Previously: Covance Catalog# MMS-466S
Isotype: Mouse IgG2a
Ave. Rating: Submit a Review
Product Citations: publications

1A4_Smooth-Muscle-Actin_Antibody_IHC_041515 — Immunohistochemical staining of Smooth Muscle Actin (1A4) Monoclonal Antibody, on formalin fixed paraffin embedded human breast tissue at 1/50 dilution.

Compare all formats

Cat #

Size

Price

Quantity Check Availability

Save

904601

250 µL

282€

Check Availability

Need larger quantities of this item?
Request Bulk Quote

This product is scheduled for discontinuation. Please use the availability checker to determine current availability. Cat. Nos. 614851 & 614852 are available as an alternative.

Description

Cytoplasmic actins, which belong to the microfilament system of cytoskeleton proteins, are some of the most conserved eukaryotic proteins being expressed in mammals and birds. The actin protein consists of six isoforms, varying in their amino acid sequence, but all having the same molecular mass of 42 kD. The isoforms show more than 90% overall sequence homology, but only 50-60% homology in their 18 N-terminal residues. The N-terminal region appears to be a major antigenic region. There are different α isoforms specific for muscle tissues, i.e. skeletal muscle α cardiac muscle α and smooth muscle α respectively. The β- and γ-actins may be present in muscle cells as well as most other cell types in the body, including non-muscle cells.

Product Details

Technical Data Sheet (pdf)

Product Details

Verified Reactivity: Human, Mouse, Rat, Cow, Chicken
Antibody Type: Monoclonal
Host Species: Mouse
Immunogen: This antibody was raised against the N-terminal synthetic decapeptide of alpha-smooth muscle actin coupled to keyhole limpet haemocyanin (KLH).
Formulation: Cell Culture Supernatant (dialysed against 0.05 mol/L Tris/HCl, pH 7.2, and containing 15 mmol/L NaN3).
Preparation: Tissue Culture Supernatant
Storage & Handling: Store between 2°C and 8°C.
Application: IHC-P, ICC, WB
Recommended Usage: Each lot of this antibody is quality control tested by immunohistochemical staining.

The optimal working dilution should be determined for each specific assay condition.
• WB: 1:100*
• IHC: 1:50-1:100**

Paraffin sections: This antibody can be used for labelling paraffin-embedded tissue sections fixed in formalin. Pre-treatment of tissues with heat-induced epitope retrieval is recommended. Optimal results are obtained with 10 mmol/L Tris buffer, 1 mmol/L EDTA, pH 9.0. Pre-treatment of tissues with proteinase-K was found destructive of the epitope. The tissue sections should not dry out during the treatment or during the following immunocytochemical staining procedure.
Frozen sections and cell preparations: The antibody can also be used for labelling acetone-fixed, frozen sections.
Application Notes: This antibody is effective in immunoblotting (WB), immunocytochemistry (ICC) and immunohistochemistry (IHC).

*Predicted MW = 42 kD

**Reccomend 20 minute heat-induced epitope retrieval in 10 mmol/L Tris buffer, 1 mmol/L EDTA, pH 9.0, and 30 minute incubation of primary antibody at room temperature.
RRID: AB_2565041 (BioLegend Cat. No. 904601)

Antigen Details

Biology Area: Cell Biology, Cell Motility/Cytoskeleton/Structure, Neuroscience, Neuroscience Cell Markers
Molecular Family: Microfilaments
Gene ID: 59 View all products for this Gene ID
UniProt: View information about Alpha Smooth Muscle Actin on UniProt.org

Documentation

Reviews

Review This Product

Related Protocols

Related Products

Description	Clone	Applications

Related Pages & Pathways

Related FAQs

Other Formats

View All Alpha Smooth Muscle Actin Reagents Request Custom Conjugation

Description	Clone	Applications
Anti-Alpha Smooth Muscle Actin	1A4	IHC-P,ICC,WB
Purified anti-α Smooth Muscle Actin	1A4	IHC-P,ICC,WB
Alexa Fluor® 488 anti-α Smooth Muscle Actin	1A4	IHC-P,ICC
Alexa Fluor® 647 anti-α Smooth Muscle Actin	1A4	IHC-P,ICC

Certificate of Analysis

Customers Also Purchased

Purified anti-Tubulin β 3 (TUBB3)

Clone: TUJ1

Ultra-LEAF™ Purified anti-c-Myc

Clone: 9E10

Purified anti-Cytokeratin (pan reactive)

Clone: C-11

Purified anti-human CD144 (VE-Cadherin)

Clone: BV9

Go To Top Version: 3 Revision Date: 04.27.2023

For Research Use Only. Not for diagnostic or therapeutic use.

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

BioLegend, the BioLegend logo, and all other trademarks are property of BioLegend, Inc. or their respective owners, and all rights are reserved.

8999 BioLegend Way, San Diego, CA 92121 www.biolegend.com
Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.