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Brilliant Violet 421™ anti-human CD161 Antibody

Pricing & Availability
Clone
HP-3G10 (See other available formats)
Regulatory Status
RUO
Other Names
NKR-P1A
Isotype
Mouse IgG1, κ
Ave. Rating
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Product Citations
publications
HP-3G10_BV421_1_112311
Human peripheral blood lymphocytes stained with CD4 FITC and CD161 (clone HP-3G10) Brilliant Violet 421™ (top) or mouse IgG1 κ Brilliant Violet 421™ isotype control (bottom).
  • HP-3G10_BV421_1_112311
    Human peripheral blood lymphocytes stained with CD4 FITC and CD161 (clone HP-3G10) Brilliant Violet 421™ (top) or mouse IgG1 κ Brilliant Violet 421™ isotype control (bottom).
  • HP-3G10_BV421_2_112311
Compare all formats See Brilliant Violet 421™ spectral data
Cat # Size Price Quantity Check Availability Save
339913 25 tests 164€
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339914 100 tests 326€
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Description

CD161 is a type II transmembrane glycoprotein, also known as NKR-P1A, that is expressed as a 40-44 kD homodimer. It is a member of the C-type lectin superfamily. CD161 is expressed on a majority of NK cells, NKT cells, and subsets of peripheral T cells and CD3+ thymocytes. It has been reported that Th17 cells are a subpopulation of CD4+CD161+CCR6+ cells. While the biological function of CD161 is not clear, it has been suggested to serve either as a stimulatory receptor or to inhibit NK cell-mediated cytotoxicity and cytokine production. LLT-1 (lectin-like transcript-1, also named as osteoclast inhibitory lectin or CLEC2D) is the ligand of CD161.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human, Cynomolgus, Rhesus
Reported Reactivity
African Green, Baboon
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Human NK cells
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA).
Preparation
The antibody was purified by affinity chromatography and conjugated with Brilliant Violet 421™ under optimal conditions.
Concentration
Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.

Brilliant Violet 421™ excites at 405 nm and emits at 421 nm. The standard bandpass filter 450/50 nm is recommended for detection. Brilliant Violet 421™ is a trademark of Sirigen Group Ltd.


Learn more about Brilliant Violet™.

This product is subject to proprietary rights of Sirigen Inc. and is made and sold under license from Sirigen Inc. The purchase of this product conveys to the buyer a non-transferable right to use the purchased product for research purposes only. This product may not be resold or incorporated in any manner into another product for resale. Any use for therapeutics or diagnostics is strictly prohibited. This product is covered by U.S. Patent(s), pending patent applications and foreign equivalents.
Excitation Laser
Violet Laser (405 nm)
Application Notes

Additional reported applications (for the relevant formats) include: inhibition of cytokine production and Western blotting under nonreducing conditions.

Application References
  1. Gumß M, et al. 2004. Blood 104:3664.
  2. Exley M, et al. 1998. J. Exp. Med. 188:867.
  3. Marquez C, et al. 1998. Blood 91:2760.
Product Citations
  1. Liu Y, et al. 2022. Signal Transduct Target Ther. 7:347. PubMed
  2. Upadhyay AA, et al. 2023. Nat Commun. 14:1914. PubMed
  3. Ruiz-Rosado Jde D, et al. 2016. Mediators Inflamm. 9101762:2016. PubMed
  4. Lu JC, et al. 2024. Nat Commun. 621:15. PubMed
  5. Wragg KM, et al. 2020. Cell Reports. 31(11):107773. PubMed
  6. Hoang TN, et al. 2021. Cell. 184:460. PubMed
  7. Hoang TN, et al. 2020. bioRxiv. . PubMed
  8. Khaitan A, et al. 2016. PLoS One. 11: 0161786. PubMed
  9. Jansen D, et al. 2015. Rheumatology. 54:728. PubMed
  10. Golebski K, et al. 2021. Immunity. 54(2):291-307.e7. PubMed
  11. Liu Y, et al. 2020. Methods Mol Biol. 2111:285. PubMed
  12. Longbrake EE, et al. 2018. Mult Scler. 24:728. PubMed
  13. Björkander S, et al. 2016. Sci Rep. 6:22083. PubMed
  14. Bal S, et al. 2016. Nat Immunol. 10.1038/ni.3444. PubMed
  15. Hackstein CP, et al. 2022. Nat Commun. 13:7472. PubMed
  16. Wiedemann A, et al. 2020. Nat Commun. 3.048611111. PubMed
  17. Dai B, et al. 2021. Cell Reports Medicine. 2(8):100381. PubMed
RRID
AB_10915270 (BioLegend Cat. No. 339913)
AB_10915270 (BioLegend Cat. No. 339914)

Antigen Details

Structure
Type II glycoprotein, 40-44 kD homodimer, C-type lectin superfamily
Distribution

NK cells, T subset, subset of CD3+ thymocytes

Ligand/Receptor
LLT-1 (Lectin-like transcript-1)
Cell Type
NK cells, T cells, Thymocytes
Biology Area
Cell Biology, Immunology, Innate Immunity, Signal Transduction
Molecular Family
CD Molecules
Antigen References

1. Takahashi T, et al. 2006. J. Immunol. 176:211.
2. Cosmi L, et al. 2008. J. Exp. Med. 205:1903.
3. Aldemir H, et al. 2005. J. Immunol. 175:7791.
4. Rosen DB, et al. 2008. J. Immunol. 180:6508.

Gene ID
3820 View all products for this Gene ID
UniProt
View information about CD161 on UniProt.org

Related FAQs

What is the F/P ratio range of our BV421™ format antibody reagents?

It is lot-specific. On average it ranges between 2-4.

Go To Top Version: 1    Revision Date: 11.30.2012

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

BioLegend, the BioLegend logo, and all other trademarks are property of BioLegend, Inc. or their respective owners, and all rights are reserved.

 

8999 BioLegend Way, San Diego, CA 92121 www.biolegend.com
Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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