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FITC anti-human CD14 Antibody

Pricing & Availability
Clone
HCD14 (See other available formats)
Regulatory Status
RUO
Other Names
LPS receptor
Isotype
Mouse IgG1, κ
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Product Citations
publications
HCD14_FITC_061407
Human peripheral blood monocytes stained with HCD14 FITC
  • HCD14_FITC_061407
    Human peripheral blood monocytes stained with HCD14 FITC
Compare all formats See FITC spectral data
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325603 25 tests 76€
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325604 100 tests 169€
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Description

CD14 is a 53-55 kD glycosylphosphatidylinositol (GPI)-linked membrane glycoprotein also known as LPS receptor. CD14 is expressed at high levels on monocytes and macrophages, and at lower levels on granulocytes. Some dendritic cell populations such as interfollicular dendritic cells, reticular dendritic cells, and Langerhans cells have also been reported to express CD14. As a high-affinity receptor for LPS, CD14 is involved in the clearance of gram-negative pathogens and in the upregulation of adhesion molecules and cytokines expression in monocytes and neutrophils.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human
Antibody Type
Monoclonal
Host Species
Mouse
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation
The antibody was purified by affinity chromatography, and conjugated with FITC under optimal conditions.
Concentration
Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.

Excitation Laser
Blue Laser (488 nm)
Application Notes

Additional reported applications (for the relevant formats) include: immunofluorescence microscopy. This clone was tested in-house and does not work on formalin fixed paraffin-embedded (FFPE) tissue.

Application References
  1. McMichael A, et al. 1987. Leucocyte Typing III. Oxford University Press. New York.
  2. Knapp W, et al. Eds. 1989. Leucocyte Typing IV. Oxford University Press. New York.
  3. Schlossman S, et al. Eds. 1995. Leucocyte Typing V. Oxford University Press. New York.
Product Citations
  1. Toepfner N et al. 2018. eLife. 7 pii: e29213. PubMed
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  13. Sykes DB et al. 2016. Cell. 167(1):171-186 . PubMed
  14. Kokkinopoulos I, et al. 2021. Sci Rep. 11:15759. PubMed
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  16. Laustsen A, et al. 2021. Elife. 10:. PubMed
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  18. Jama B, Morris G 2014. J Vis Exp. 93: 52257. PubMed
  19. Esaulova E, et al. 2020. Cell Host Microbe. 29(2):165-178.e8. PubMed
  20. Švajger U, et al. 2009. Immunology. 129:525. PubMed
  21. Hansen IS, et al. 2018. Nat Commun. 9:863. PubMed
  22. Domingos-Pereira S, et al. 2019. Cancer Immunol Res. 7:621. PubMed
  23. Walk J, et al. 2019. Nat Commun. 10:874. PubMed
  24. BÖiers C, et al. 2018. Dev Cell. 44:362. PubMed
  25. Zhang P, et al. 2022. BMC Med. 20:435. PubMed
  26. Feng T, et al. 2022. Nat Commun. 13:5208. PubMed
  27. Chen IP, et al. 2020. Bio Protoc. 10:e3854. PubMed
  28. Shen X, et al. 2020. Molecular Cell. 80(6):1013-1024.e6. PubMed
  29. Mangsbo S, et al. 2009. J Immunol. 183:6724. PubMed
  30. Mandl M, et al. 2014. PLoS One. 9:112140. PubMed
  31. Warmuth S, et al. 2022. Oncoimmunology. 10:2004661. PubMed
  32. Wißfeld J, et al. 2021. Glia. 69:1393. PubMed
  33. Huang Y, et al. 2008. J Immunol. 180:2367. PubMed
  34. Huang H, et al. 2022. Nat Commun. 13:5925. PubMed
  35. Wang F, et al. 2021. Cell. 184(2):422-440.e17. PubMed
  36. Timmerman K, et al. 2008. J Leukoc Biol. 84:1271. PubMed
  37. Hazenberg MD, et al. 2019. Blood Adv. 2.659722222. PubMed
  38. Lu J, et al. 2021. Front Immunol. 12:732612. PubMed
  39. Rossignol ED, et al. 2021. Cell Reports. 35(8):109167. PubMed
  40. Georgakis S, et al. 2021. JCI Insight. 6:. PubMed
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  56. Tan EE, et al. 2020. J Clin Invest. 130:5817. PubMed
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RRID
AB_830677 (BioLegend Cat. No. 325603)
AB_830677 (BioLegend Cat. No. 325604)

Antigen Details

Structure
GPI-linked membrane glycoprotein, 53-55 kD
Distribution

Monocytes, macrophages, granulocytes (low)

Function
LPS receptor, clearance of Gram-negative pathogens
Ligand/Receptor
LPS
Cell Type
Granulocytes, Macrophages, Monocytes, Neutrophils
Biology Area
Cell Biology, Immunology, Innate Immunity, Neuroinflammation, Neuroscience
Molecular Family
CD Molecules
Antigen References

1. Stocks S, et al. 1990. Biochem. J. 268:275.
2. Wright S, et al. 1990. Science 249:1434.

Gene ID
929 View all products for this Gene ID
UniProt
View information about CD14 on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 2    Revision Date: 07.13.2015

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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