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Purified anti-T-bet Antibody

Pricing & Availability
Clone
4B10 (See other available formats)
Regulatory Status
RUO
Other Names
T-box expressed in T cells, T box 21, TBLYM
Isotype
Mouse IgG1, κ
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Product Citations
publications
1_4B10_PURE_T-Bet_Antibody_1_WB_103116.JPG
Total cell lysate from PBMC (lane 1, 15 µg) and PBMC treated with 5 µg/mL CD3 and 2 µg/mL CD28 (lane 2, 15 µg) were resolved by electrophoresis (4-12% Bis-Tris), transferred to nitrocellulose, and probed with purified anti-T-bet antibody (clone 4B10). Proteins were visualized using an HRP Goat anti-mouse IgG Antibody and chemiluminescence detection. Direct-Blot™ HRP anti-β-actin antibody (clone 2F1-1) was used as a loading control.
  • 1_4B10_PURE_T-Bet_Antibody_1_WB_103116.JPG
    Total cell lysate from PBMC (lane 1, 15 µg) and PBMC treated with 5 µg/mL CD3 and 2 µg/mL CD28 (lane 2, 15 µg) were resolved by electrophoresis (4-12% Bis-Tris), transferred to nitrocellulose, and probed with purified anti-T-bet antibody (clone 4B10). Proteins were visualized using an HRP Goat anti-mouse IgG Antibody and chemiluminescence detection. Direct-Blot™ HRP anti-β-actin antibody (clone 2F1-1) was used as a loading control.
  • 2_4B10_PURE_T-Bet_Antibody_2_ICFC_080619
    Human peripheral blood lymphocytes were surface stained with CD3 (clone OKT3) APC and then treated with True-Nuclear™ Transcription Factor Buffer Set. Cells were then stained with purified T-bet (clone 4B10) (left) or purified mouse IgG1, κ isotype control (right), followed by anti-mouse IgG1 PE.
  • 3_4B10_PURE_T-Bet_Antibody_WB_2_081417
    Total lysates (15µg protein) from Daudi, K562 and NK-92 cells were resolved by electrophoresis (4-20% Tris-glycine gel), transferred to nitrocellulose, and probed with 1:500 diluted (1µg/mL) purified anti- T-bet antibody (4B10, upper). Proteins were visualized using an HRP goat anti-mouse-IgG secondary antibody and chemiluminescence detection. Direct-Blot™ HRP anti-β-actin Antibody was used as a loading control (lower). Lane M: Molecular weight ladder.
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644801 25 µg 81€
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644802 100 µg 177€
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Description

T-bet, also known as T-box transcription factor T-bet, is considered to be a "master regulator" of Th1 lymphoid development controlling the production of the cytokine IFN-γ. T-bet is widely expressed in hematopoietic cells including stem cells, NK cells, B cells, and T cells. T-bet is critical for the control of microbial pathogens, and knockout animals show multiple physiologic and inflammatory features characteristic of asthma. T-bet expression is optimally observed after IL-12 stimulation and can be suppressed by addition of the Th2 cytokine IL-4 or neutralization of IL-12.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human, Mouse
Antibody Type
Monoclonal
Host Species
Mouse
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

WB, ICFC - Quality tested
IHC-F, IP - Reported in the literature, not verified in house
Recommended Usage

Each lot of this antibody is quality control tested by Western blotting and intracelluar immunofluorescent staining using our True-Nuclear™ Transcription Factor Staining Protocol. For Western blotting, the suggested use is 1.0 to 2.0 µg per ml. For flow cytometric staining, the suggested use of this reagent is 1.0 µg per million cells in a staining volume of 100 µl. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

Additional reported applications (for the relevant formats) include: immunoprecipitation2 and immunofluorescence microscopy3.

NOTE: For flow cytometric staining with this clone, True-Nuclear™ Transcription Factor Buffer Set (Cat. No. 424401) offers improved staining and is highly recommended over the Foxp3 Fix/Perm Buffer Set (Cat. No. 421403) and the Nuclear Factor Fixation and Permeabilization Buffer Set (Cat. No. 422601).

Application References
  1. Szabo SJ, et al. 2000. Cell 100:655. (ICFC, WB)
  2. Hwang ES, et al. 2005. J. Exp. Med. 202:1289. (ICFC, WB, IP)
  3. Neurath MF, et al. 2002. J. Exp. Med. 195:1129. (IF)
  4. Hsieh CY, et al. 2012. J Pharmacol Exp. 343:125. PubMed.
Product Citations
  1. Arnold IC, et al. 2019. PLoS Pathog. 15:e1007866. PubMed
  2. McCarthy EE, et al. 2022. Cell Rep. 39:110815. PubMed
  3. Yang Z, et al. 2022. Cancer Discov. 12:1942. PubMed
  4. Poon MML, et al. 2023. Nat Immunol. 24:309. PubMed
  5. Seifert HA, et al. 2018. Metab Brain Dis. 33:1599. PubMed
  6. Stankovic S, et al. 2015. J Immunol. 195: 4892 - 4899. PubMed
  7. Wang D, et al. 2018. Immunity. 48:659. PubMed
  8. Arnold IC, et al. 2018. J Exp Med. 215:2055. PubMed
  9. Yang J, et al. 2020. Front Immunol. 10:3048. PubMed
  10. Draganov D, et al. 2021. NPJ Breast Cancer. 7:22. PubMed
  11. Chen J, et al. 2020. J Immunol. 204:2076. PubMed
  12. Wang Y, et al. 2021. Cancer Cell. 39:1375. PubMed
  13. Mayer KA, et al. 2021. FASEB J. 35:e21217. PubMed
  14. Hilligan KL, et al. 2020. Nat Commun. 11:5637. PubMed
  15. Zhao L, et al. 2018. Nat Med. 24:1536. PubMed
  16. Manni M, et al. 2018. Nat Immunol. 1.074305556. PubMed
  17. Hsieh C, et al. 2012. J Pharmacol Exp Ther. 343:125. PubMed
  18. Liu F, et al. 2020. J Immunother Cancer. 8:. PubMed
  19. Yang K, et al. 2017. Nature. 548:602. PubMed
  20. O'Boyle KC, et al. 2020. Methods Mol Biol. 2111:1. PubMed
  21. Sprouse ML, et al. 2018. JCI Insight. 3:e97322. PubMed
  22. Tao H, et al. 2021. Front Immunol. 12:623280. PubMed
  23. Eccles JD, et al. 2020. Cell Rep. 30:351. PubMed
  24. Ejima R, et al. 2021. Nutrients. 13:. PubMed
  25. Parigi SM, et al. 2018. Sci Rep. 0.440277778. PubMed
  26. Kakaradov B, et al. 2017. Nat Immunol. 18:422. PubMed
  27. Singh AK, et al. 2017. Front Immunol. 0.513888889. PubMed
  28. Newell KL, et al. 2021. PLoS One. 16:e0244855. PubMed
  29. Roussey JA, et al. 2017. J Immunol. 199:3535. PubMed
  30. Wang D, et al. 2022. EMBO Rep. 23:e53691. PubMed
  31. Scortegagna M, et al. 2020. Nat Commun. 11:99. PubMed
  32. Stelekati E, et al. 2018. Cell Rep. 2.445833333. PubMed
  33. Lee B, et al. 2019. Front Immunol. 0.561805556. PubMed
  34. Dey P, et al. 2020. Cancer Discov. 10:608. PubMed
  35. Jenks SA et al. 2018. Immunity. 49(4):725-739 . PubMed
  36. Sasawatari S, et al. 2020. J Immunol. 1373:204. PubMed
RRID
AB_1595608 (BioLegend Cat. No. 644801)
AB_1595608 (BioLegend Cat. No. 644802)

Antigen Details

Structure
T-box transcription factor, approximately 58 kD.
Distribution

Nuclear; expressed in T cells, hematopoietic stem cells, NK cells, B cells, lung, spleen.

Function
Th1-specific T-box transcription factor controlling expression of the hallmark Th1 cytokine, interferon gamma (IFN-γ). T-bet expression is critical for the control of microbial pathogens.
Cell Type
B cells, Hematopoietic stem and progenitors, NK cells, T cells, Tregs
Biology Area
Cell Biology, Immunology, Transcription Factors
Molecular Family
Nuclear Markers
Antigen References

1. Szabo SJ, et al. 2000. Cell 100:655.
2. Szabo SJ, et al. 2002. Science 295:338.
3. Finotto S, et al. 2002. Science 295:336.
4. Mullen AC, et al. 2001. Science 292:1907.

Gene ID
30009 View all products for this Gene ID
UniProt
View information about T-bet on UniProt.org
Go To Top Version: 7    Revision Date: 08.06.2019

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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