Cell-Vive™ CD Cell Staining Buffer, GMP is an antibody diluent and cell wash buffer optimized for staining of cell suspensions. It is formulated to reduce non-specific binding of antibodies and interference with indirect staining techniques such as biotin/avidin.
Cell viability (A) was tested on PBMCs incubated in Cell-Vive™ CD Cell Staining Buffer or benchmark staining buffer for 3h at 4°C and analyzed using flow cytometry. Non-specific binding (B) and specific binding (C) of antibodies was tested on PBMCs stained with PE rat IgG2b, ĸ isotype control antibody (clone RTK4530) or PE anti-human HLA-DR (clone LN3) respectively. Flow cytometry stain was performed in presence of Cell-Vive™ CD Cell Staining Buffer, benchmark, or PBS.
Ensure the purity of isolated, enriched cells with Cell-Vive™ Ultra-LEAF™ Defined Human TruStain FcX™, GMP. It effectively blocks the unwanted and non-specific staining caused by FcR-Fc interactions without interfering with specific Fab antibody-mediated staining. Our first-to-market GMP FcR blocking buffer is chemically-defined and serum-free, removing reliance on serum while maintaining control and consistency during characterization and potency analysis of desired cells.
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