Loading...

7-AAD Viability Staining Solution

Pricing & Availability
Regulatory Status
RUO
Other Names
Viability dye, dead cell exclusion dye, cell viability dye
Ave. Rating
Submit a Review
Product Citations
publications
See 7-AAD spectral data
Cat # Size Price Quantity Check Availability Save
420403 200 tests 30 CHF
Check Availability


Need larger quantities of this item?
Request Bulk Quote
420404 500 tests 59 CHF
Check Availability


Need larger quantities of this item?
Request Bulk Quote
Description

7-AAD (7-amino-actinomycin D) has a high DNA binding constant and is efficiently excluded by intact cells. It is useful for DNA analysis and dead cell discrimination during flow cytometric analysis. When excited by 488 laser light, 7-AAD fluorescence is detected in the far red range of the spectrum (650 nm long-pass filter).

Product Details
Technical Data Sheet (pdf)

Product Details

Formulation
PBS, pH 7.2 with 0.09% (w/v) sodium azide.
Preparation
CAUTION: 7-AAD is a potential carcinogen. It is recommended that the user wear protective clothing, gloves, and eye/face protection in order to avoid contact with skin and eyes.
Concentration
50 µg/ml
Storage & Handling
Protect from light. Store between 2°C and 8°C.
Application

FC - Quality tested
Recommended Usage

For dead cell exclusion, resuspend cell pellet in 0.5 mL of Cell Staining Buffer and add 5 µl of 7-AAD per million cells and incubate for 5-10 minutes in the dark before analysis. The analysis of the cells should be done as soon as possible after the incubation with the dye.

We recommend utilizing our Fluorescence Spectra Analyzer to determine compatibility of 7AAD with other fluorochromes.

 

Excitation Laser
Blue Laser (488 nm)
Application Notes

7-AAD Viability Staining Solution can be used as a viability probe for methods of nonviable cell exclusion.

Additional Product Notes

View more applications data for this product in our Scientific Poster Library.

Application References

(PubMed link indicates BioLegend citation)
  1. Paterson AM, et al. 2011. J. Immunol. 187:1097. PubMed
  2. Wingren M, et al. 2012. Arterioscler Thromb Vasc Biol. 32:2000. PubMed
  3. Sandu SK, et al. 2012. PNAS 109:20047. PubMed
  4. Evdokimova V, et al. 2012. Sci Signal. 5:92. PubMed
  5. Choudhury KR, et al. 2012. PLoS One. 7:e51415. PubMed
  6. Wu Y, et al. 2013. J. Immunol. 190:1859. PubMed
  7. Harker KS, et al. 2013. J. Leukoc Biol. 93:789. PubMed
  8. Zhang ZN, et al. 2013. Clin Chem. 59:1175. PubMed
  9. Mikhak Z, et al. 2013. J Exp Med. 210:1855. PubMed
  10. Cohen HB, et al. 2013. Blood. 122:1935. PubMed
  11. Hufbauer M, et al. 2013. J. Virol. 87:12158. PubMed
  12. Turner JE, et al. 2103. J Exp Med. PubMed
  13. Teng T, et al. 2013. Mol Cell Biol. 33:4660. PubMed
  14. Hussain MS, et al. 2013. Hum Mol Genet. 22:5199. PubMed
  15. Turner JE, et al. 2013 J. Exp Med. 210:2951. PubMed
  16. Kong W, et al. 2014. Cell Immunol. 287:1. PubMed
  17. Fell LH, et al. 2014. Nephrol Dial Transplant. PubMed
  18. Kraaji MD, et al. 2014. Cytokine. 67:7. PubMed
  19. Schneider C, et al. 2014. PNAS. 111:8185. PubMed
  20. Ermann J, et al. 2014. PNAS. 111:2559. PubMed
  21. Cao L, et al. 2014. J Virol. 88:8656. PubMed
  22. Ni PP, et al. 2014. J Immunol. 193:1778. PubMed
  23. Wedel J, et al. 2014. J Leukoc Biol. 96:453. PubMed
  24. Jaworska J, et al. 2015. J Immunol. 194:325. PubMed
Product Citations
  1. Kulpa DA, et al. 2019. J Virol. 93:e00969-19. PubMed
  2. Van Loenhout J, et al. 2019. Cancers (Basel). 1.567361111. PubMed
  3. Dobie R, et al. 2020. Cell Reports. 29(7):1832-1847.e8.. PubMed
  4. Jiang G, et al. 2020. Aging (Albany NY). 12:11466. PubMed
  5. Dokun A, et al. 2015. Am J Physiol Heart Circ Physiol. 309: 790-803. PubMed
  6. Kitazawa M, et al. 2017. PLoS One. 10.1371/journal.pone.0169340. PubMed
  7. McCaw L, et al. 2017. EBioMedicine. 15:24-35. PubMed
  8. Mirlekar B, et al. 2020. Cancer Immunol Res. 0.536111111. PubMed
  9. de Winde CM, et al. 2021. iScience. 24:102976. PubMed
  10. Lawson H, et al. 2021. Stem Cell Reports. 16:2784. PubMed
  11. Nagamachi A, et al. 2021. J Clin Invest. 131:. PubMed
  12. Zhang T, et al. 2022. EBioMedicine. 77:103872. PubMed
  13. Tabynov K, et al. 2022. NPJ Vaccines. 7:24. PubMed
  14. Inclan-Rico JM, et al. 2020. Nat Immunol. 21:1181. PubMed
  15. Doughty D, et al. 2022. Exp Biol Med (Maywood). 247:1433. PubMed
  16. Tátrai P, et al. 2022. EMBO J. 41:e108739. PubMed
  17. Wanrooy BJ, et al. 2022. Immunol Cell Biol. 100:482. PubMed
  18. Li D, et al. 2022. Cell Prolif. 55:e13299. PubMed
  19. Obermeier M, et al. 2022. Front Cell Dev Biol. 10:891173. PubMed
  20. Xie H, et al. 2022. Nucleic Acids Res. 50:7479. PubMed
  21. Ugamraj HS, et al. 2022. MAbs. 14:2095949. PubMed
  22. Millar FR, et al. 2022. Cell Rep. 41:111596. PubMed
  23. Alula KM, et al. 2023. Sci Rep. 13:18. PubMed
  24. Henning AN, et al. 2023. iScience. 26:105801. PubMed
  25. Nandi I, et al. 2023. J Clin Invest. :. PubMed
  26. Shen JZ, et al. 2022. Mol Cell. 82:1123. PubMed
  27. Zhao X, et al. 2022. Cell Mol Immunol. 19:540. PubMed
  28. Ajay AK, et al. 2022. Cell Rep. 38:110473. PubMed
  29. Knelson EH, et al. 2022. Cancer Immunol Res. 10:947. PubMed
  30. An S, et al. 2022. Int J Mol Sci. 23:. PubMed
  31. Wu R, et al. 2022. Nat Immunol. 23:1536. PubMed
  32. Manolakou T, et al. 2022. Sci Adv. 8:eabo5840. PubMed
  33. Basile A, et al. 2023. Antiviral Res. 211:105546. PubMed
  34. Zhao Q, et al. 2023. Signal Transduct Target Ther. 8:40. PubMed
  35. Li L, et al. 2023. iScience. 26:106095. PubMed
  36. Siatra P, et al. 2023. NPJ Regen Med. 8:13. PubMed
  37. Li G, et al. 2023. Cell Death Dis. 14:185. PubMed
  38. Dada S, et al. 2023. Front Immunol. 13:982082. PubMed
  39. Hu Y, et al. 2023. Front Immunol. 14:1128423. PubMed
  40. Pang JM, et al. 2023. PLoS One. 18:e0284744. PubMed
  41. Duan JL, et al. 2023. Nat Aging. 3:258. PubMed
  42. Feng Y, et al. 2023. J Transl Med. 21:290. PubMed
  43. Simon T, et al. 2023. Front Neurosci. 17:1125492. PubMed
  44. Pinho AG, et al. 2023. Int J Mol Sci. 24:. PubMed
  45. Petenkova A, et al. 2023. Nat Commun. 14:2761. PubMed
  46. Ishida K, et al. 2023. Front Immunol. 14:1111729. PubMed
  47. Bayat H, et al. 2023. Mol Ther Nucleic Acids. 32:432. PubMed
  48. George M, et al. 2023. Cells. 12:. PubMed
  49. McKenzie C, et al. 2023. Mol Ther Nucleic Acids. 32:603. PubMed
  50. Sarno J, et al. 2023. Nat Commun. 14:2935. PubMed
  51. He J, et al. 2023. Biomolecules. 13:. PubMed
  52. Imai H, et al. 2023. iScience. 26:106822. PubMed
  53. Golubovskaya V, et al. 2023. Cancers (Basel). 15:. PubMed
  54. Shibata K, et al. 2022. Nat Commun. 13:6948. PubMed
  55. Sim SL, et al. 2022. Front Endocrinol (Lausanne). 13:957833. PubMed
  56. Zhao Q, et al. 2022. Nat Commun. 13:648. PubMed
  57. Bader AS, et al. 2021. DNA Repair (Amst). 105:103158. PubMed
  58. Pan J, et al. 2021. Mol Ther Nucleic Acids. 26:970. PubMed
  59. Glass DR, et al. 2020. Immunity. 53:217. PubMed
  60. Hilliard K, et al. 2015. Infect Immun . 83: 4015 - 4027. PubMed
  61. Walz S, et al. 2015. Blood. 126: 1203-1213. PubMed
  62. Kraaij M, et al. 2014. Cytokine. 67:7. PubMed
  63. Iida T, et al. 2019. Cell Mol Gastroenterol Hepatol. 9:277. PubMed
  64. Kuljanin M, et al. 2018. Cell Rep. 25:2524. PubMed
  65. Wu SJ, et al. 2017. Cell Rep. 2.628472222. PubMed
  66. Saleh MM, et al. 2019. Cell Host Microbe. 25:756. PubMed
  67. Feng W, et al. 2017. Nat Commun. 0.697916667. PubMed
  68. Nixon AML, et al. 2019. Sci Rep. 0.959722222. PubMed
  69. Sifuentes–Dominguez LF, et al. 2019. Elife. 8:e49910. PubMed
  70. Kobayashi T, et al. 2019. Cell. 176:982. PubMed
  71. Hussain M, et al. 2013. Hum Mol Genet. 122:1935. PubMed
  72. Turner J, et al. 2013. J Exp Med. 210:2951. PubMed
  73. Schmidt J, et al. 2015. J Immunol Methods. 423: 12-17. PubMed
  74. Paterson A, et al. 2015. J Exp Med. 212: 1603-1621. PubMed
  75. Campana R, et al. 2015. J Allergy Clin Immunol. pii: S0091-6749(15)01331-7. PubMed
  76. Lee Y, et al. 2016. Am J Physiol Regul Integr Comp Physiol. 310: R55 - R65. PubMed
  77. Nagafuchi Y, et al. 2016. Sci Rep. 6:29338. PubMed
  78. Liu S, et al. 2017. PLoS One. 10.1371/journal.pone.0169701. PubMed
  79. Zhang J, et al. 2021. MedComm (Beijing). 2:256. PubMed
  80. Wang Q, et al. 2022. Nat Commun. 13:3022. PubMed
  81. Park JH, et al. 2022. STAR Protoc. 3:101607. PubMed
  82. Hoffmann K, et al. 2022. Commun Biol. 5:875. PubMed
  83. Steen CB, et al. 2021. Cancer Cell. 39:1422. PubMed
  84. Kim Y, et al. 2020. J Neurochem. 155:177. PubMed
  85. Liu Y, et al. 2022. Cancer Discov. :. PubMed
  86. Hamel Y, et al. 2021. Cell Rep Med. 2:100370. PubMed
  87. Nagatake T, et al. 2022. Mucosal Immunol. 15:289. PubMed
  88. Kimura I, et al. 2022. Cell Rep. 38:110218. PubMed
  89. Menzel L, et al. 2021. Cell Rep. 37:109878. PubMed
  90. Stringhini M, et al. 2021. MAbs. 13:1868066. PubMed
  91. Leimkühler NB, et al. 2020. Cell Stem Cell. 28:637. PubMed
  92. Devalaraja S, et al. 2020. Cell. 1098:180. PubMed
  93. Hirano KI, et al. 2021. eLife. 0.416666666666667. PubMed
  94. Rennier K, et al. 2020. Clin Cancer Res. 26:5019. PubMed
  95. Mahauad-Fernandez W, Okeoma C 2017. Cell Death Dis. 10.1038/cddis.2017.68. PubMed
  96. Coppo M, et al. 2016. Nat Commun. 7:12254. PubMed
  97. Lindgren I, et al. 2016. Hum Reprod. 31: 672 - 683. PubMed
  98. Fuchs R, et al. 2015. Toxicology. 338: 17-29. PubMed
  99. Wedel J, et al. 2014. J Leukoc Biol. 96:453. PubMed
  100. Ermann J, et al. 2014. Proc Natl Acad Sci U S A. 111:2559. PubMed
  101. Bowen ME, et al. 2019. Dev Cell. 50:212. PubMed
  102. Hanzawa N, et al. 2020. Sci Rep. 10:5181. PubMed
  103. Wanrooy BJ, et al. 2018. J Neuroinflammation. 0.828472222. PubMed
  104. Fortin J, et al. 2020. Cancer Cell. 37:308. PubMed
  105. Tomlinson JE, et al. 2020. PLoS Pathog. 16:e1008677. PubMed
  106. Evdokimova V, et al. 2012. Sci Signal. 5:92. PubMed
  107. Zhang Z, et al. 2013. Clin Chem. 59:1175. PubMed
  108. Mahiti M, et al. 2015. Biochem Biophys Res Commun. 463: Pags 248-254. PubMed
  109. Leung T, et al. 2015. Genes Dev. 29: 2097 - 2107.. PubMed
  110. Palm A, et al. 2016. Sci Rep. 10.1038/srep20531. PubMed
  111. Alikhan M, et al. 2016. J Am Soc Nephrol. 27: 706 - 714. PubMed
  112. Figueroa-Valdés AI, et al. 2021. Front Bioeng Biotechnol. 9:619930. PubMed
  113. Song C, et al. 2022. Stem Cell Res Ther. 13:48. PubMed
  114. Papafragkos I, et al. 2022. Front Immunol. 13:889075. PubMed
  115. Yin W, et al. 2022. Biomolecules. 12:. PubMed
  116. González-Jiménez A, et al. 2022. Int J Mol Sci. 23: . PubMed
  117. Fan X, et al. 2021. Genome Biol. 22:195. PubMed
  118. Teng ZX, et al. 2022. J Inflamm Res. 15:3187. PubMed
  119. Maity S, et al. 2022. Front Immunol. 13:818266. PubMed
  120. Shahzadi SK, et al. 2022. Clin Sci (Lond). 136:181. PubMed
  121. Kolbrink B, et al. 2022. Biochem J. 479:259. PubMed
  122. Hu W, et al. 2021. Vaccines (Basel). 9:. PubMed
  123. Wang Z, et al. 2020. Autophagy. 1:. PubMed
  124. Abe S, et al. 2020. JCI Insight. 5:00. PubMed
  125. Urbanska K, et al. 2015. Cancer Immunol Res. 3: 1130 - 1137. PubMed
  126. Ni P, et al. 2014. J Immunol . 193:1778. PubMed
  127. Hussain M, et al. 2013. Hum Mol Genet. 22:5199. PubMed
  128. Saika A, et al. 2020. FASEB Bioadv. 2:59. PubMed
  129. Pan J, et al. 2020. J Neuroinflammation. 17:97. PubMed
  130. Naqvi N,et al. 2017. Sci Rep.. 10.1038/s41598-017-12753-1. PubMed
  131. Kho D, et al. 2017. PLoS One. 10.1371/journal.pone.0180267. PubMed
  132. Sheng Y, et al. 2019. Cell Prolif. 52:e12611. PubMed
  133. Tang Z, et al. 2019. Cell Rep. 28:1744. PubMed
  134. Schneider C, et al. 2014. Proc Natl Acad Sci U S A. 111:8185. PubMed
  135. Baker A, et al. 2016. Cancer Res . 76: 1158 - 1169. PubMed
  136. Nkwouano V, et al. 2017. PLoS One. 12(2):e0171817. PubMed
  137. Laughney AM, et al. 2020. Nat Med. 26:259. PubMed
  138. Verma B, et al. 2020. Curr Protoc Pharmacol. 89:e77. PubMed
  139. Devalaraja S, et al. 2020. STAR Protoc. 1:100188. PubMed
  140. Sheahan BJ, et al. 2021. Cell Mol Gastroenterol Hepatol. 12:119. PubMed
  141. Hintz HM, et al. 2021. Cancer Immunol Res. 9:1270. PubMed
  142. Paluri SL, et al. 2021. DNA Repair (Amst). 105:103152. PubMed
  143. Zhao F, et al. 2022. Front Immunol. 13:873720. PubMed
  144. Uceda-Castro R, et al. 2022. Cell Rep Med. 3:100821. PubMed
  145. Ghoochani A, et al. 2021. Cancer Res. 81:1583. PubMed
  146. Martín Monreal MT, et al. 2021. Front Immunol. 12:716250. PubMed
  147. Vonk WIM, et al. 2020. Mol Cell. 329:78. PubMed
  148. Aregger M, et al. 2020. Nat Metab. 0.429861111. PubMed
  149. Perales-Puchalt A, et al. 2017. Clin Cancer Res. 23(2):441-453. PubMed
  150. Qi X, et al. 2016. Mucosal Immunol. 10.1038/mi.2016.25. PubMed
  151. Harker K, et al. 2013. J Leukoc Biol. 93:789. PubMed
  152. Choudhury K, et al. 2012. PLoS One. 7:e51415. PubMed
  153. Zhou L, et al. 2020. Clin Cancer Res. 26:290. PubMed
  154. Buus TB, et al. 2018. Blood Adv. 1.552083333. PubMed
  155. Krishnasamy K,et al. 2017. Nat Commun. . 10.1038/s41467-017-00953-2. PubMed
  156. Sogkas G, et al. 2017. Inflammation. 10.1007/s10753-017-0661-y. PubMed
  157. Cabron AS, et al. 2018. J Immunol. 201:3106. PubMed
  158. Sharif T, et al. 2018. Autophagy. 1.101388889. PubMed
  159. Ramstead AG, et al. 2020. Cell Rep. 30:2889. PubMed
  160. Jaworska K, et al. 2015. J Immunol. 194:325. PubMed
  161. Ligtenberg M, et al. 2016. J Immunol. 196: 759 - 766. PubMed
  162. Peiris T, et al. 2016. Development. 143: 1697 - 1709.. PubMed
  163. Lehmann M, et al. 2016. J Leukoc Biol. 99: 1057 - 1064. PubMed
  164. Hrdinka M, et al. 2016. PLoS One. 11: 0162863. PubMed
  165. P, et al. 2016. Mol Ther. 24: 1602-1614. PubMed
  166. Chimote A, et al. 2017. Cancer Res . 77(1):53-61. PubMed
  167. Vis B, et al. 2020. Proc Natl Acad Sci U S A. 117:285. PubMed
  168. Zhang F, et al. 2020. EMBO Mol Med. e12034:12. PubMed
  169. Hanasoge Somasundara AV, et al. 2021. Cell Rep. 37:110099. PubMed
  170. Gao S, et al. 2022. Cell Res. 32:38. PubMed
  171. Ganguly K, et al. 2022. Front Immunol. 13:930449. PubMed
  172. Kunitomi A, et al. 2022. Cell Rep Methods. 2:100317. PubMed
  173. Ding Y, et al. 2022. Cell Death Dis. 13:996. PubMed
  174. Robilliard LD, et al. 2021. Immunol Cell Biol. 99:403. PubMed
  175. Zeng Q, et al. 2022. Front Immunol. 13:740805. PubMed
  176. Kumar A, et al. 2021. STAR Protoc. 2:100874. PubMed
  177. Jakic B, et al. 2021. Cell Death Dis. 12:187. PubMed
  178. Kang MH, et al. 2020. Nat Commun. 3.186805556. PubMed
  179. Kadowaki A, et al. 2016. Nat Commun. 7:11639. PubMed
  180. Cao L, et al. 2014. J Virol. 88:8656. PubMed
  181. Hofmann M, et al. 2012. Leukemia. 26:1288. PubMed
  182. Teo YV, et al. 2019. Cell Rep. 27:997. PubMed
  183. Sun H, et al. 2017. J Autoimmun. 10.1016/j.jaut.2017.08.004. PubMed
  184. Jain RW, et al. 2018. Cell Rep. 25:3342. PubMed
  185. Lobb I, et al. 2015. J Urol. In Press, Uncorrected Proof,. PubMed
  186. Suarez S, et al. 2015. J Biol Chem. 290: 28311 - 28320. PubMed
  187. Han W, et al. 2015. Stem Cell Dev. 24: 2536-2546. PubMed
  188. Marsh T, et al. 2016. Cancer Res . 76: 2932 - 2943. PubMed
  189. Nosho S, et al. 2020. Int J Mol Sci. 21:00. PubMed
  190. Caeser R, et al. 2021. Nat Protoc. 16:2499. PubMed
  191. França TT, et al. 2021. JCI Insight. 6:. PubMed
  192. Nakasone H, et al. 2021. Commun Biol. 4:1177. PubMed
  193. Huang HC, et al. 2021. EBioMedicine. 74:103712. PubMed
  194. Jorapur A, et al. 2022. PLoS Pathog. 18:e1010200. PubMed
  195. Sharma GP, et al. 2022. Int J Radiat Oncol Biol Phys. . PubMed
  196. Brennan CA, et al. 2021. Gut Microbes. 13:1987780. PubMed
  197. Kolbrink B, et al. 2022. Cell Mol Life Sci. 79:387. PubMed
  198. He C, et al. 2022. BMC Cancer. 22:1100. PubMed
  199. Zimmerli D, et al. 2022. Nat Commun. 13:6579. PubMed
  200. Nasser H, et al. 2022. STAR Protoc. 3:101773. PubMed
  201. Rotolo A, et al. 2021. STAR Protoc. 2:100905. PubMed
  202. Clement CC, et al. 2021. Nat Commun. 12:4447. PubMed
  203. Lin A, et al. 2021. Cancers (Basel). 13:. PubMed
  204. Tamò L, et al. 2021. Int J Mol Sci. 22:00. PubMed
  205. Parayath NN, et al. 2020. Nat Commun. 4.680555556. PubMed
  206. Galle-Treger L, et al. 2016. Nat Commun. 7:13202. PubMed
  207. Ihara S, et al. 2016. J Immunol. 196: 4603 - 4613. PubMed
  208. Kumai T, et al. 2015. Sci Rep. 5:16280. PubMed
  209. Teng T, et al. 2013. Mol Cell Biol. 33:4660. PubMed
  210. Kundu K, et al. 2019. Cancer Immunol Res. 1.069444444. PubMed
  211. Qiao J, et al. 2019. Cancer Cell. 35:901. PubMed
  212. Yoshioka T, et al. 2017. PLoS One. 12:e0174103. PubMed
  213. Olson WJ, et al. 2019. Cell Rep. 28:2878. PubMed
  214. Kurachi M, et al. 2017. Nat Protoc. 10.1038/nprot.2017.083. PubMed
  215. Li B, et al. 2019. Oncol Rep. 41:608. PubMed
  216. Combes F, et al. 2019. Cancers (Basel). 1.675. PubMed
  217. Good Z, et al. 2019. Nat Biotechnol. 37:259. PubMed
  218. Gebhardt C, et al. 2015. Clin Cancer Res. 21: 5453 - 5459. PubMed
  219. Eipper S, et al. 2016. PLoS One. 11: 0151509. PubMed
  220. Kariminia A, et al. 2016. Blood. 127: 3082 - 3091. PubMed
  221. Wu X, et al. 2016. Proc Natl Acad Sci U S A. 113(51):14775-14780. PubMed
  222. Malara A, et al. 2017. J Biol Chem. 292(8):3239-3251. PubMed
  223. Lan H, et al. 2020. Int Immunol. 559:32. PubMed
  224. Alam Z, et al. 2020. Cell Rep. 107825:31. PubMed
  225. Zhong MC, et al. 2021. J Exp Med. 218:. PubMed
  226. Roy AG, et al. 2021. Int J Mol Sci. 22:. PubMed
  227. Alasady MJ, et al. 2021. PLoS One. 16:e0256238. PubMed
  228. Mikhailova V, et al. 2022. Int J Mol Sci. 23:. PubMed
  229. Zhang R, et al. 2022. Front Pharmacol. 13:870848. PubMed
  230. Chen C, et al. 2021. J Virol. 95:e0082921. PubMed
  231. Liang G, et al. 2021. STAR Protoc. 2:100884. PubMed
  232. Bello A, et al. 2021. Immunol Cell Biol. 99:879. PubMed
  233. Matsuda T, et al. 2022. iScience. 25:105324. PubMed
  234. Saber M, et al. 2021. J Neurosci Res. 99:1136. PubMed
  235. Mirlekar B, et al. 2022. Cell Rep Med. :100744. PubMed
  236. Shibuya M, et al. 2021. iScience. 24:103131. PubMed
  237. Hirata SI, et al. 2020. Allergy. 75:1939. PubMed
  238. Burgener SS, et al. 2019. Cell Rep. 27:3646. PubMed
  239. Park CG, et al. 2018. Sci Transl Med. 10:eaar1916. PubMed
  240. Celik H, et al. 2018. Cancer Cell. 34:741. PubMed
  241. Duan Y, et al. 2019. Cell Death Dis. 0.948611111. PubMed
  242. Muroski M, et al. 2016. PLoS One. 11: 0145129. PubMed
  243. Ligon MM, et al. 2020. Mucosal Immunol. 1.172222222. PubMed
  244. Wang M, et al. 2020. Oncogene. 39:399. PubMed
  245. Tulasi DY, et al. 2021. Cell Mol Gastroenterol Hepatol. 11:1437. PubMed
  246. Hoffmann J, et al. 2021. Nat Commun. 12:3964. PubMed
  247. NULL, et al. 2022. Cell. 185:916. PubMed
  248. Colli LM, et al. 2021. Am J Hum Genet. 108:1590. PubMed
  249. Seki N, et al. 2022. iScience. 25:104838. PubMed
  250. Fabre P, et al. 2022. Curr Protoc. 2:e356. PubMed
  251. Leary N, et al. 2022. J Extracell Vesicles. 11:e12197. PubMed
  252. Oya Y, et al. 2021. Front Immunol. 12:779709. PubMed
  253. Burnett DL, et al. 2021. Immunity. S1074-7613:00457. PubMed
  254. Kiss M, et al. 2020. Cancer Immunol Res. 9:309. PubMed
  255. Lin T, et al. 2021. PLoS Pathog. 17:e1009557. PubMed
  256. Lei L, et al. 2021. Front Cell Dev Biol. 659744:9. PubMed
  257. Yan P, et al. 2020. Cell Rep. 31:107840. PubMed
  258. Dingjan I, et al. 2016. Sci Rep. 6:22064. PubMed
  259. Kamimura D, et al. 2015. Int Immunol. 10.1093/intimm/dxv059. PubMed
  260. Otsubo D, et al. 2015. Anticancer Res. 35: 4425-4431. PubMed
  261. Fell L, et al. 2014. Nephrol Dial Transplant. 29:809. PubMed
  262. Hufbauer M, et al. 2013. J Virol. 87:12158. PubMed
  263. Koury J, et al. 2019. PLoS One. 14:e0218670. PubMed
  264. Chapelin F, et al. 2017. Sci Rep.. 10.1038/s41598-017-17669-4. PubMed
  265. Jacobs R, et al. 2017. J Immunol Methods. . 10.1016/j.jim.2017.09.001. PubMed
  266. Woolf N, et al. 2017. Oncogenesis. 10.1038/oncsis.2017.77. PubMed
  267. Thomas A, et al. 2017. PLoS One.. 10.1371/journal.pone.0183542. PubMed
  268. Sandhu S, et al. 2012. Proc Natl Acad Sci U S A. 109:20047. PubMed
  269. Quantius J, et al. 2016. PLoS One. 12: 1005544. PubMed
  270. Festag J, et al. 2020. Mol Ther Nucleic Acids. 1.330555556. PubMed
  271. Aggio-Bruce R, et al. 2020. Mol Neurobiol. . PubMed
  272. Sakurai Y, et al. 2021. Pharmaceutics. 13:. PubMed
  273. Wang F, et al. 2021. Genomics Proteomics Bioinformatics. 19:208. PubMed
  274. Iraqi M, et al. 2022. Int J Mol Sci. 23:. PubMed
  275. Walle T, et al. 2022. Sci Adv. 8:eabh4050. PubMed
  276. Liu Z, et al. 2022. JCI Insight. Online ahead of print. PubMed
  277. Tran NL, et al. 2022. Elife. 11:. PubMed
  278. Pan J, et al. 2022. Front Immunol. 13:812924. PubMed
  279. Rochford I, et al. 2021. Am J Physiol Lung Cell Mol Physiol. 321:L686. PubMed
  280. Li Y, et al. 2021. Front Genet. 12:679616. PubMed
  281. Chetty A, et al. 2021. Cell Host Microbe. 29:579. PubMed
  282. Kurashima Y, et al. 2021. Nat Commun. 12:1067. PubMed
  283. Sharma R, et al. 2021. J Neuroinflammation. 72:18. PubMed
  284. Dyson MR, et al. 2020. MAbs. 12:1829335. PubMed
  285. Dewdney B, et al. 2020. Sci Rep. 10:16769. PubMed
  286. Gomez J, et al. 2016. J Immunol. 197: 2864 - 2879. PubMed
  287. Palm A, Friedrich H, Kleinau S 2016. Sci Rep. 6:27687. PubMed
  288. Bosma M, et al. 2016. Nat Commun. 7: 11314. PubMed
  289. Catarinella M, et al. 2016. EMBO Mol Med. 8: 155 - 170. PubMed
  290. Paterson A, et al. 2011. J Immunol. 187:1097. PubMed
  291. Ajith A, et al. 2019. FASEB J. 33:5220. PubMed
  292. Han P, et al. 2020. Sci Adv. 6:eaaz1580. PubMed
  293. Pedro L, et al. 2019. Small. 15:e1900224. PubMed
  294. Rosenberger L, et al. 2019. Sci Rep. 0.835416667. PubMed
  295. Contreras O, et al. 2018. Skelet Muscle. 8:05. PubMed
  296. Tarumoto Y, et al. 2020. Blood. 135:56:00. PubMed
  297. Ho J, et al. 2011. PLoS One. 6:e16815. PubMed
  298. Wu Y, et al. 2013. J Immunol. 190:1859. PubMed
  299. Walachowski S, et al. 2016. PLoS One. 11: 0148464. PubMed
  300. Urso K, et al. 2016. PLoS One. 11: 0158893. PubMed
  301. Corrado M, et al. 2020. Cell Metab. 32:981. PubMed
  302. Das A, et al. 2020. J Bone Miner Res. 36:199. PubMed
  303. Chen C, et al. 2022. iScience. 25:103645. PubMed
  304. Xu L, et al. 2022. Front Immunol. 13:841141. PubMed
  305. Sheng W, et al. 2021. Cancer Discov. 11:1970. PubMed
  306. Lulu AM, et al. 2021. Cancer Immunol Res. 9:1327. PubMed
  307. Xin Z, et al. 2022. Nat Commun. 13:5463. PubMed
  308. Özçelik E, et al. 2022. PLoS One. 17:e0275476. PubMed
  309. Jiang L, et al. 2022. Front Cell Neurosci. 16:992869. PubMed
  310. Moritsch S, et al. 2022. Oncoimmunology. 11:2127271. PubMed
  311. Motozono C, et al. 2022. Nat Commun. 13:5440. PubMed
  312. Scheyltjens I, et al. 2022. Nat Protoc. 17:2354. PubMed
  313. Pieterse Z, et al. 2022. Methods Mol Biol. 2429:435. PubMed
  314. Shankman LS, et al. 2021. Curr Biol. 31:2469. PubMed
  315. Song X, et al. 2022. Transl Oncol. 15:101306. PubMed
  316. Liu Y, et al. 2021. Nat Commun. 12:6831. PubMed
  317. Fasolino M, et al. 2020. Immunity. 257:52. PubMed
  318. Guan H, et al. 2015. Oncogenesis. 4: 166. PubMed
  319. Bonaccorsi-Riani E, et al. 2015. PLoS One. 10: 0136106. PubMed
  320. Kong W, et al. 2014. Cell Immunol. 287:1. PubMed
  321. Mikhak Z, et al. 2013. J Exp Med. 210:1855. PubMed
  322. Wigren M, et al. 2012. Arterioscler Thromb Vasc Biol. 32:2000 . PubMed
  323. Sfakianos JP, et al. 2020. Nat Commun. 2.222222222. PubMed
  324. Golubovskaya V,et al. 2017. Cancers (Basel). . 10.3390/cancers9100139. PubMed

Antigen Details

Biology Area
Cell Biology, Cell Cycle/DNA Replication, Cell Proliferation and Viability, Neuroscience
Antigen References

1. Schmid I, et al. 1992. Cytometry. 13:204

Gene ID
NA
Specificity (DOES NOT SHOW ON TDS):
7-AAD
Specificity Alt (DOES NOT SHOW ON TDS):
7-AAD
App Abbreviation (DOES NOT SHOW ON TDS):
FC

Related FAQs

Why is washing not recommended after the addition 7-AAD or PI addition when assessing viability?

These dyes bind in equilibrium with DNA. Therefore, external dye concentration must be maintained during analysis and the dye should not be washed out.

Go To Top Version: 2    Revision Date: 05.21.2014

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

BioLegend, the BioLegend logo, and all other trademarks are property of BioLegend, Inc. or their respective owners, and all rights are reserved.

 

8999 BioLegend Way, San Diego, CA 92121 www.biolegend.com
Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

ProductsHere
Login / Register
Remember me
Forgot your password? Reset password?
Create an Account