APC/Fire 750 anti-mouse/human CD45R/B220 Antibody anti-CD45R

Pricing & Availability

Clone: RA3-6B2 (See other available formats)
Regulatory Status: RUO
Other Names: B220
Isotype: Rat IgG2a, κ
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Product Citations: publications

RA3-6B2_APCFire750_CD45R_B220_Antibody_1_030816 — C57BL/6 mouse splenocytes were stained with CD3 PE and B220 (clone RA3-6B2) APC/Fire™ 750 (top) or rat IgG2a, κ APC/Fire™ 750 isotype control (bottom).

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Size

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103259

25 µg

112 CHF

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103260

100 µg

229 CHF

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Description

CD45R, also known as B220, is an isoform of CD45. It is a member of the protein tyrosine phosphatase (PTP) family with a molecular weight of approximately 180-240 kD. CD45R is expressed on B cells (at all developmental stages from pro-B cells through mature B cells), activated B cells, and subsets of T and NK cells. CD45R (B220) is also expressed on a subset of abnormal T cells involved in the pathogenesis of systemic autoimmunity in MRL-Fas^lpr and MRL-Fas^gld mice. It plays a critical role in TCR and BCR signaling. The primary ligands for CD45 are galectin-1, CD2, CD3, and CD4. CD45R is commonly used as a pan-B cell marker; however, CD19 may be more appropriate for B cell specificity.

Product Details

Technical Data Sheet (pdf)

Product Details

Verified Reactivity

Mouse, Human

Reported Reactivity

Cat

Antibody Type

Monoclonal

Host Species

Rat

Immunogen

Abelson murine leukemia virus-induced pre-B tumor cells

Formulation

Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.

Preparation

The antibody was purified by affinity chromatography and conjugated with APC/Fire™ 750 under optimal conditions.

Concentration

0.2 mg/ml

Storage & Handling

The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.

Application

FC - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.5 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

* APC/Fire™ 750 has a maximum excitation of 650 nm and a maximum emission of 774 nm.

Application Notes

Clone RA3-6B2 has been described to react with an epitope on the extracellular domain of the transmembrane CD45 glycoprotein which is dependent upon the expression of exon A and specific carbohydrate residues. Additional reported applications (for the relevant formats) include: immunoprecipitation¹, in vitro and in vivo modulation of B cell responses^2-4, immunohistochemistry of acetone-fixed frozen sections and formalin-fixed paraffin-embedded sections^5,6, and spatial biology (IBEX)^14,15.

Application References (PubMed link indicates BioLegend citation)

Coffman RL. 1982. Immunol. Rev. 69:5. (IP)
George A, et al. 1994. J. Immunol. 152:1014. (Activ)
Asensi V, et al. 1989. Immunology 68:204. (Activ)
Domiati-Saad R, et al. 1993. J. Immunol. 151:5936. (Activ)
Hata H, et al. 2004. J. Clin. Invest. 114:582. (IHC)
Monteith CE, et al. 1996. Can. J. Vet. Res. 60:193. (IHC)
Shih FF, et al. 2006. J. Immunol. 176:3438. (FC)
Chang C L-T, et al. 2007. J. Immunol. 178:6984.
Fazilleau N, et al. 2007. Nature Immunol. 8:753.
Lang GL, et al. 2008. Blood 111:2158. PubMed
Charles N, et al. 2010. Nat. Med. 16:701. (FC) PubMed
del Rio ML, et al. 2011. Transpl. Int. 24:501. (FC) PubMed
Murakami R, et al. 2013. PLoS One. 8:73270. PubMed
Radtke AJ, et al. 2020. Proc Natl Acad Sci U S A. 117:33455-65. (SB) PubMed
Radtke AJ, et al. 2022. Nat Protoc. 17:378-401. (SB) PubMed

Product Citations

Shenoy AT, et al. 2022. Cytometry A. 101:892. PubMed
Yeh CH, et al. 2022. Immunity. 55:272. PubMed
Kodali S, et al. 2022. J Immunol. 208:1085. PubMed
Weigert A, et al. 2022. Nat Commun. 13:6078. PubMed
Chen JS, et al. 2022. Sci Immunol. 7:eabl5652. PubMed
Gomez S, et al. 2022. J Immunother Cancer. 10:. PubMed
Teng F, et al. 2021. Cell Rep. 37:110051. PubMed
Dumitrescu M, et al. 2020. Int J Mol Sci. 21:00. PubMed
Goc J, et al. 2021. Cell. 184:5015. PubMed

RRID

AB_2572108 (BioLegend Cat. No. 103259)
AB_2572108 (BioLegend Cat. No. 103260)

Antigen Details

Structure: Protein tyrosine phosphatase (PTP) family, 180-240 kD
Distribution: B cells, T cell subset, NK cell subset
Function: Phosphatase, T and B cell activation
Ligand/Receptor: Galectin-1, CD2, CD3, CD4
Cell Type: B cells, NK cells, T cells
Biology Area: Cell Biology, Immunology, Inhibitory Molecules, Neuroscience, Neuroscience Cell Markers
Molecular Family: CD Molecules
Antigen References: 1. Barclay A, et al. 1997. The Leukocyte Antigen FactsBook Academic Press.
2. Trowbridge IS, et al. 1993. Annu. Rev. Immunol. 12:85.
3. Kishihara K, et al. 1993. Cell 74:143.
4. Pulido R, et al. 1988. J. Immunol. 140:3851.
Gene ID: 19264 View all products for this Gene ID 5788 View all products for this Gene ID
Specificity (DOES NOT SHOW ON TDS):: CD45R
Specificity Alt (DOES NOT SHOW ON TDS):: CD45R/B220
App Abbreviation (DOES NOT SHOW ON TDS):: FC
UniProt: View information about CD45R on UniProt.org

Documentation

Reviews

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Related Protocols

Cell Surface Flow Cytometry Staining Protocol

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Description	Clone	Applications
Alexa Fluor® 594 anti-mouse/human CD45R/B220	RA3-6B2	IHC-F,FC,3D IHC
APC anti-mouse/human CD45R/B220	RA3-6B2	FC
Biotin anti-mouse/human CD45R/B220	RA3-6B2	FC,IHC-F
FITC anti-mouse/human CD45R/B220	RA3-6B2	FC
PE anti-mouse/human CD45R/B220	RA3-6B2	FC
PE/Cyanine5 anti-mouse/human CD45R/B220	RA3-6B2	FC
Purified anti-mouse/human CD45R/B220	RA3-6B2	FC,CyTOF®,IHC-F,IHC-P,IP,Activ
PE/Cyanine7 anti-mouse/human CD45R/B220	RA3-6B2	FC
APC/Cyanine7 anti-mouse/human CD45R/B220	RA3-6B2	FC
Alexa Fluor® 488 anti-mouse/human CD45R/B220	RA3-6B2	FC,IHC-F,3D IHC
Alexa Fluor® 647 anti-mouse/human CD45R/B220	RA3-6B2	FC,IHC-F,3D IHC,SB
Pacific Blue™ anti-mouse/human CD45R/B220	RA3-6B2	FC
Alexa Fluor® 700 anti-mouse/human CD45R/B220	RA3-6B2	FC
PerCP anti-mouse/human CD45R/B220	RA3-6B2	FC
PerCP/Cyanine5.5 anti-mouse/human CD45R/B220	RA3-6B2	FC
Brilliant Violet 421™ anti-mouse/human CD45R/B220	RA3-6B2	FC,IHC-F
Brilliant Violet 570™ anti-mouse/human CD45R/B220	RA3-6B2	FC
Brilliant Violet 650™ anti-mouse/human CD45R/B220	RA3-6B2	FC
Brilliant Violet 605™ anti-mouse/human CD45R/B220	RA3-6B2	FC
Brilliant Violet 785™ anti-mouse/human CD45R/B220	RA3-6B2	FC
Brilliant Violet 510™ anti-mouse/human CD45R/B220	RA3-6B2	FC,IHC-F,SB
Purified anti-mouse/human CD45R/B220 (Maxpar® Ready)	RA3-6B2	FC,CyTOF®
Brilliant Violet 711™ anti-mouse/human CD45R/B220	RA3-6B2	FC
PE/Dazzle™ 594 anti-mouse/human CD45R/B220	RA3-6B2	FC
APC/Fire™ 750 anti-mouse/human CD45R/B220	RA3-6B2	FC
Brilliant Violet 750™ anti-mouse/human CD45R/B220	RA3-6B2	FC
TotalSeq™-A0103 anti-mouse/human CD45R/B220	RA3-6B2	PG
Spark Blue™ 550 anti-mouse/human CD45R/B220	RA3-6B2	FC
Spark NIR™ 685 anti-mouse/human CD45R/B220	RA3-6B2	FC
TotalSeq™-B0103 anti-mouse/human CD45R/B220	RA3-6B2	PG
Ultra-LEAF™ Purified anti-mouse/human CD45R/B220	RA3-6B2	FC,CyTOF®,IHC-F,IHC-P,IP,Activ
TotalSeq™-C0103 anti-mouse/human CD45R/B220	RA3-6B2	PG
PE/Fire™ 640 anti-mouse/human CD45R/B220	RA3-6B2	FC
APC/Fire™ 810 anti-mouse/human CD45R/B220	RA3-6B2	FC
PE/Fire™ 700 anti-mouse/human CD45R/B220	RA3-6B2	FC
Spark Violet™ 538 anti-mouse/human CD45R/B220	RA3-6B2	FC
Spark YG™ 581 anti-mouse/human CD45R/B220	RA3-6B2	FC
Spark YG™ 570 anti-mouse/human CD45R/B220	RA3-6B2	IHC-F
PE/Fire™ 810 anti-mouse/human CD45R/B220	RA3-6B2	FC
Spark Blue™ 574 anti-mouse/human CD45R/B220 Antibody	RA3-6B2	FC
Spark Violet™ 423 anti-mouse/human CD45R/B220 Antibody	RA3-6B2	FC
Spark Red™ 718 anti-mouse/human CD45R/B220	RA3-6B2	FC
Spark UV™ 387 anti-mouse/human CD45R/B220	RA3-6B2	FC
Spark PLUS UV395™ anti-mouse/human CD45R/B220	RA3-6B2	FC

Certificate of Analysis

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Go To Top Version: 1 Revision Date: 03.15.2016

For Research Use Only. Not for diagnostic or therapeutic use.

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