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Biotin anti-mouse IgG2a Antibody

Pricing & Availability
Clone
RMG2a-62 (See other available formats)
Regulatory Status
RUO
Other Names
Immunoglobulin G2a
Isotype
Rat IgG
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Product Citations
publications
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407103 50 µg 83 CHF
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407104 500 µg 206 CHF
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Description

The RMG2a-62 monoclonal antibody reacts with immunoglobulin G2a and G2c (IgG2a, IgG2c) and in all tested mouse haplotype (Igh-a and b). It does not react with other isotypes. The RMG2a-62 monoclonal antibody may be used as primary or secondary reagent for ELISA or immunofluorescent analysis.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Mouse Ig cocktail
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography, and conjugated with biotin under optimal conditions.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C. Do not freeze.
Application

ELISA, FC
Recommended Usage

Each lot of this antibody is quality control tested by ELISA. For ELISA detection, the suggested use of this reagent is ≤ 1.0 µg per ml. It's recommended that the reagent be titrated for optimal performance for each application.

Application References

(PubMed link indicates BioLegend citation)
  1. Gavin AL, et al.2006. Science 314:1936.
  2. Podolin PL, et al. 2008. J. Immunol. 180:7989. (IP) PubMed
Product Citations
  1. Cao K, et al. 2022. J Virol. 96:e0157821. PubMed
  2. Babayeva M, et al. 2022. Front Immunol. 13:983621. PubMed
  3. Pattnaik A, et al. 2023. Vaccines (Basel). 11:. PubMed
  4. He X, et al. 2021. Emerg Microbes Infect. 10:1555. PubMed
  5. Ray A, et al. 2015. PLoS One. 10: 0137314. PubMed
  6. Matsuda T, et al. 2022. iScience. 25:105324. PubMed
  7. Soto JA, et al. 2018. Front Immunol. 9:2875. PubMed
  8. Dahlgren M, et al. 2015. J Immunol. 194:5187. PubMed
RRID
AB_345323 (BioLegend Cat. No. 407103)
AB_345323 (BioLegend Cat. No. 407104)

Antigen Details

Gene ID
668478 View all products for this Gene ID
Specificity (DOES NOT SHOW ON TDS):
IgG2a
Specificity Alt (DOES NOT SHOW ON TDS):
IgG2a
App Abbreviation (DOES NOT SHOW ON TDS):
ELISA,FC
UniProt
View information about IgG2a on UniProt.org

Related FAQs

IgG2a gene is deleted in some mouse strains, which ones are they?

IgG2a gene is deleted in C57Bl/6, C57Bl/10, SJL, and NOD mice. It is replaced with IgG2c gene instead.

How many biotin molecules are per antibody structure?
We don't routinely measure the number of biotins with our antibody products but the number of biotin molecules range from 3-6 molecules per antibody.
Go To Top Version: 3    Revision Date: 02.16.2015

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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