Brilliant Violet 421™ anti-human/mouse Granzyme B Recombinant Antibody

Product Details

Verified Reactivity

Human, Mouse

Antibody Type

Recombinant

Host Species

Rat

Formulation

Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA).

Preparation

The antibody was purified by affinity chromatography and conjugated with Brilliant Violet 421™ under optimal conditions.

Concentration

Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)

Storage & Handling

The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.

Application

ICFC - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by intracellular immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.

Brilliant Violet 421™ excites at 405 nm and emits at 421 nm. The standard bandpass filter 450/50 nm is recommended for detection. Brilliant Violet 421™ is a trademark of Sirigen Group Ltd.

Learn more about Brilliant Violet™.

This product is subject to proprietary rights of Sirigen Inc. and is made and sold under license from Sirigen Inc. The purchase of this product conveys to the buyer a non-transferable right to use the purchased product for research purposes only. This product may not be resold or incorporated in any manner into another product for resale. Any use for therapeutics or diagnostics is strictly prohibited. This product is covered by U.S. Patent(s), pending patent applications and foreign equivalents.

Excitation Laser

Violet Laser (405 nm)

Application Notes

Clone QA18A28 is compatible with Fixation buffer (Cat. No. 420801) and Intracellular Staining Permeabilization Wash Buffer (Cat. No. 421002) in both C57BL/6 splenocytes and human PBMC. QA18A28 is also compatible with True-Nuclear™ Transcription Factor Buffer Set (Cat. No. 424401).

Product Citations

1. Wang F, et al. 2023. Int J Mol Sci. 24:. PubMed
2. Lin CH, et al. 2023. iScience. 26:106904. PubMed
3. Yang QC, et al. 2023. iScience. 26:106916. PubMed
4. Jiang XL, et al. 2021. Nat Commun. 12:897. PubMed
5. Wu J, et al. 2023. Bioact Mater. 20:259. PubMed
6. Liang Y, et al. 2022. Theranostics. 12:7729. PubMed
7. Xie Z, et al. 2022. Cell Discov. 8:133. PubMed
8. Lin YH 2023. Immunity. 56(1):207-223.e8. PubMed
9. Jin SM, et al. 2023. Nat Nanotechnol. :. PubMed
10. Peng W, et al. 2023. Cells. 12: . PubMed
11. Toomer G, et al. 2022. Viruses. 14:. PubMed
12. Duan H, et al. 2021. J Clin Invest. 131:. PubMed
13. Hirschberger S, et al. 2022. Front Med (Lausanne). 9:923502. PubMed
14. Zhang X, et al. 2021. Nat Commun. 12:4536. PubMed
15. Hirschberger S, et al. 2021. EMBO Mol Med. 13:e14323. PubMed
16. Shuwa HA, et al. 2021. Med. 2(6):720-735.e4. PubMed
17. Wilson TL, et al. 2022. Cancer Discov. 12:2098. PubMed
18. Wang F, et al. 2021. Neoplasia. 23:281. PubMed

RRID

AB_2810603 (BioLegend Cat. No. 396413)
AB_2810603 (BioLegend Cat. No. 396414)

Antigen Details

Structure

32 kD serine protease

Distribution

Cytotoxic T cells, NK cells, and neutrophils, low on granulocytes, B cells and activated dendritic cells

Function

Granzyme B is able to induce target cell apoptosis by activating caspase independent pathways. Granzyme B is induced in CD8⁺ T lymphocytes with ConA/ IL-2 and CD4⁺ T lymphocytes with anti CD3/CD28 or CD3/CD46.

Interaction

Caspase-3

Ligand/Receptor

Mannose-6-phosphate receptor

Cell Type

Neutrophils, T cells

Biology Area

Cell Biology, Immunology, Innate Immunity, Neuroscience

Molecular Family

Enzymes and Regulators, Proteases

Antigen References

1. Estebanez-Perpina E, et al. 2000. Biol Chem. 381:1203.
2. Griffiths GM. And S. Isaaz, et al. 1993. J. Cell Biol. 120:885.
3. Spaeny-Dekking EH, et al. 1998. J. Immunol. 160:3610.
4. Wagner C, et al. 2008. Mol. Immunol. 45:1761.

Gene ID

3002 View all products for this Gene ID 14939 View all products for this Gene ID

Specificity (DOES NOT SHOW ON TDS):

Granzyme B

Specificity Alt (DOES NOT SHOW ON TDS):

Granzyme B

App Abbreviation (DOES NOT SHOW ON TDS):

ICFC

UniProt

View information about Granzyme B on UniProt.org

Documentation

• Technical Data Sheet (pdf)
• Certificate of Analysis
• Safety Data Sheet

Reviews

Related Protocols

• Intracellular Flow Cytometry Staining Protocol

Related Pages & Pathways

Related FAQs

What is the F/P ratio range of our BV421™ format antibody reagents?

It is lot-specific. On average it ranges between 2-4.

Other Formats

Certificate of Analysis