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Brilliant Violet 510™ anti-mouse I-A/I-E Antibody

Pricing & Availability
Clone
M5/114.15.2 (See other available formats)
Regulatory Status
RUO
Other Names
MHC class II
Isotype
Rat IgG2b, κ
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Product Citations
publications
M5114.15.2_BV510_IAIE_Antibody_FC_091712
C57BL/6 mouse splenocytes were stained with mouse I-A/I-E (clone M5/114.15.2) Brilliant Violet 510™ (filled histogram) or rat IgG2b, κ Brilliant Violet 510™ isotype control (open histogram).
  • M5114.15.2_BV510_IAIE_Antibody_FC_091712
    C57BL/6 mouse splenocytes were stained with mouse I-A/I-E (clone M5/114.15.2) Brilliant Violet 510™ (filled histogram) or rat IgG2b, κ Brilliant Violet 510™ isotype control (open histogram).
Compare all formats See Brilliant Violet 510™ spectral data
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107635 125 µL 176 CHF
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107636 50 µg 268 CHF
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Description

These class II molecules are expressed on antigen presenting cells (including B cells) and a subset of T cells from H-2b,d,q,r bearing mice and are involved in antigen presentation to T cells expressing CD3/TCR and CD4 proteins.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Activated C57BL/6 mouse spleen cells
Formulation
µg & µl: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA).
Preparation
µg & µl: The antibody was purified by affinity chromatography and conjugated with Brilliant Violet 510™ under optimal conditions.
Concentration
µg sizes: 0.2 mg/mL
µL sizes: lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling
µg & µl: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining using µg size, the suggested use of this reagent is ≤ 0.5 µg per million cells in 100 µl volume. For flow cytometric staining using µl size, the suggested use of this reagent is 5 µl per million cells or 5 µl per 100 µl of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.

Brilliant Violet 510™ excites at 405 nm and emits at 510 nm. The bandpass filter 510/50 nm is recommended for detection, although filter optimization may be required depending on other fluorophores used. Be sure to verify that your cytometer configuration and software setup are appropriate for detecting this channel. Refer to your instrument manual or manufacturer for support. Brilliant Violet 510™ is a trademark of Sirigen Group Ltd.


Learn more about Brilliant Violet™.

This product is subject to proprietary rights of Sirigen Inc. and is made and sold under license from Sirigen Inc. The purchase of this product conveys to the buyer a non-transferable right to use the purchased product for research purposes only. This product may not be resold or incorporated in any manner into another product for resale. Any use for therapeutics or diagnostics is strictly prohibited. This product is covered by U.S. Patent(s), pending patent applications and foreign equivalents.
Excitation Laser
Violet Laser (405 nm)
Application Notes

The M5/114.15.2 antibody reacts with a polymorphic determinant shared by the I-Ab, I-Ad, I-Aq, I-Ed, and I-Ek MHC class II alloantigens from mice carrying H-2p,r,q,b,d,u haplotypes. Clone M5/114.15.2 however does not react wtih I-Af, I-Ak, or I-As MHC class II alloantigens.1

Additional reported applications (for the relevant formats) include: immunoprecipitation1, immunohistochemistry of frozen sections2,3,6, in vitro and in vivo blocking of antigen presentation or ligand binding4-7, and spatial biology (IBEX)17,18. The Ultra-LEAF™ purified antibody (Endotoxin < 0.01 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (Cat. Nos. 107655 & 107656).

Application References

(PubMed link indicates BioLegend citation)
  1. Bhattacharya A, et al. 1981. J. Immunol. 127:2488. (IP)
  2. Viville S, et al. 1993. Cell 72:635. (IHC)
  3. Nelson AJ, et al. 1993. J. Immunol. 151:2453. (IHC)
  4. Shi Y, et al. 1998. J. Exp. Med. 187:367. (Block)
  5. Yamashita I, et al. 1993. Int. Immunol. 5:1139.
  6. Guo M, et al. 1995. Zygote 3:65. (IHC)
  7. Kim A, et al. 2004. Exp. Mol. Med. 36:428. (Block)
  8. Luckashenak NA, et al. 2006. J. Immunol. 177:5177.
  9. Venanzi ES, et al. 2007. J. Immunol. 179:5693.
  10. Christensen SR, et al. 2006. Immunity 25:417. PubMed
  11. Matte-Martone C, et al. 2008. Blood 111:3884. PubMed
  12. De Pascalis R, et al. 2008. Infect. Immun. 76:4311. PubMed
  13. Kuns RD, et al. 2009. Blood 113:5999. PubMed
  14. Sabatino JJ, et al. 2011. J. Exp. Med. 208:81. PubMed
  15. Draber P, et al. 2011. Mol Cell Biol. 22:4550. PubMed
  16. Fu H, et al. 2014. Nat Commun. 5:3436. PubMed
  17. Radtke AJ, et al. 2020. Proc Natl Acad Sci U S A. 117:33455-65. (SB) PubMed
  18. Radtke AJ, et al. 2022. Nat Protoc. 17:378-401. (SB) PubMed
Product Citations
  1. Gogoi H, et al. 2020. Front Immunol. 1.620833333. PubMed
  2. Tan J, et al. 2021. iScience. 24(8):102835. PubMed
  3. Pierson M, et al. 2021. Curr Protoc. 1:e53. PubMed
  4. Dolfi B, et al. 2022. Cell Rep. 39:110949. PubMed
  5. Mansouri S, et al. 2022. J Immunol. 209:2114. PubMed
  6. Grenov A, et al. 2022. Cell Rep. 39:110778. PubMed
  7. Wu R, et al. 2022. Nat Immunol. 23:1536. PubMed
  8. Hao H, et al. 2023. Nat Biomed Eng. . PubMed
  9. Prasad S, et al. 2023. iScience. 26:106628. PubMed
  10. van Os BW, et al. 2023. Front Cardiovasc Med. 10:1171764. PubMed
  11. Wang X, et al. 2023. Stem Cell Rev Rep. . PubMed
  12. Wenzek C, et al. 2022. iScience. 25:105540. PubMed
  13. Lefebvre MN, et al. 2021. Cell Rep. 37:109956. PubMed
  14. Kashyap AS, et al. 2019. Cell Rep. 28:3367. PubMed
  15. Yu K, et al. 2021. Cell Reports. 35(12):109273. PubMed
  16. Ogbechi J, et al. 2022. Front Immunol. 13:1001956. PubMed
  17. Diao L, et al. 2022. iScience. 25:105511. PubMed
  18. Brandt S, et al. 2020. J Am Soc Nephrol. 31:2589. PubMed
  19. Yousif AS, et al. 2020. Immunity. 54(2):235-246.e5. PubMed
  20. Chikina AS, et al. 2020. Cell. 183:411. PubMed
  21. Zhu X, et al. 2017. Arch Oral Biol. 10.1016/j.archoralbio.2017.03.010. PubMed
  22. Yuzhu Hou et al. 2018. Immunity. 49(3):490-503 . PubMed
  23. Eddy W, et al. 2017. The Journal of Immunology. 10.4049/jimmunol.1601777. PubMed
  24. Moreau HD et al. 2019. Dev Cell. 49(2):171-188 . PubMed
  25. Rao S, et al. 2017. Cell. 168(3):503-516.e12. PubMed
  26. Si Y, et al. 2020. Sci Adv. 6:eaba0995. PubMed
  27. Esmaili S, et al. 2021. Cell Systems. 12(5):432-445.e7. PubMed
  28. Perner C, et al. 2020. Immunity. 53(5):1063-1077.e7. PubMed
  29. Huggins MA, et al. 2019. Cell Rep. 28:1729. PubMed
  30. Kuhn NF, et al. 2020. Nat Commun. 4.74375. PubMed
  31. Devalaraja S, et al. 2020. STAR Protoc. 1:100188. PubMed
  32. Wu X, et al. 2021. Elife. 10:. PubMed
  33. Salei N, et al. 2020. J Am Soc Nephrol. 31:257. PubMed
  34. Stump CT, et al. 2021. Open Biol. 11:210245. PubMed
  35. Sato R, et al. 2020. Int Immunol. 499:32. PubMed
  36. Liu D et al. 2019. Immunity. 51(1):64-76 . PubMed
  37. Kelsey E Sivick et al. 2018. Cell reports. 25(11):3074-3085 . PubMed
  38. Lim HY et al. 2018. Immunity. 49(2):326-341 . PubMed
  39. Leylek R, et al. 2019. Cell Rep. 29:3736. PubMed
  40. Baasch S, et al. 2021. Cell. . PubMed
  41. Garcia LR, et al. 2021. Nat Commun. 12:3364. PubMed
  42. Huang YL, et al. 2021. Commun Biol. 4:229. PubMed
  43. Mao FY, et al. 2021. Cell Mol Gastroenterol Hepatol. 12:395. PubMed
  44. Formaglio P, et al. 2021. Immunity. 54:2724. PubMed
  45. Kennedy EM, et al. 2022. Nat Commun. 13:5907. PubMed
  46. Sangesland M, et al. 2020. Cell Reports. 32(8):108065. PubMed
  47. Chauveau L, et al. 2021. EMBO Rep. 22:e52447. PubMed
  48. Scur M, et al. 2022. Nat Commun. 13:7272. PubMed
  49. Monaghan KL, et al. 2020. J Vis Exp. . PubMed
  50. Lammers K, et al. 2015. PLoS One. 10: 0138338. PubMed
  51. Ermert D, et al. 2016. PLoS Pathog. 11: 1005043. PubMed
  52. Stifter SA, et al. 2019. Cell Rep. 29:3539. PubMed
  53. Liu S, et al. 2018. Front Immunol. 29:208. PubMed
  54. Alam Z, et al. 2020. Cell Rep. 107825:31. PubMed
  55. Gamrekelashvili J, et al. 2021. Bio Protoc. 11:e4007. PubMed
  56. Mansouri S, et al. 2020. Mucosal Immunol. 0.954861111. PubMed
  57. Hammer A, et al. 2017. Front Immunol. . 10.3389/fimmu.2017.01922. PubMed
  58. Hollern DP, et al. 2020. Cell. 179(5):1191-1206.e21.. PubMed
  59. Yasuda T, et al. 2016. PLoS One. 11:e0167952. PubMed
  60. Li E, et al. 2021. Front Immunol. 12:667177. PubMed
  61. Rosato PC, et al. 2019. Nat Commun. 10:567. PubMed
  62. Velazquez VM, et al. 2017. Mol Ther Methods Clin Dev. 0.277083333. PubMed
  63. Knuplez E, et al. 2021. British Journal of Pharmacology. 178(5):1234-1248. PubMed
  64. Katzmarski N, et al. 2021. Nat Immunol. 22:1382. PubMed
  65. Truckenbrod EN, et al. 2021. Elife. 10:. PubMed
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RRID
AB_2561397 (BioLegend Cat. No. 107635)
AB_2561397 (BioLegend Cat. No. 107636)

Antigen Details

Structure
MHC class II
Distribution

B cell and activated T cells, APCs of the H-2b,d,q,r bearing mice

Function
Antigen presentation
Ligand/Receptor
CD3/TCR, CD4
Cell Type
Antigen-presenting cells, B cells, Dendritic cells, T cells, Tregs
Biology Area
Immunology, Innate Immunity
Molecular Family
MHC Antigens
Antigen References

1. Watts C. 1997. Ann. Rev. Immunol. 15:821.
2. Pamer E, et al. 1998. Ann. Rev. Immunol. 16:323.

Gene ID
14961 View all products for this Gene ID 14969 View all products for this Gene ID
Specificity (DOES NOT SHOW ON TDS):
I-A/I-E
Specificity Alt (DOES NOT SHOW ON TDS):
I-A/I-E
App Abbreviation (DOES NOT SHOW ON TDS):
FC
UniProt
View information about I-A/I-E on UniProt.org

Other Formats

View All I-A/I-E Reagents Request Custom Conjugation
Description Clone Applications
Biotin anti-mouse I-A/I-E M5/114.15.2 FC
FITC anti-mouse I-A/I-E M5/114.15.2 FC
PE anti-mouse I-A/I-E M5/114.15.2 FC
Purified anti-mouse I-A/I-E M5/114.15.2 FC,IHC-F,IP,Block
PE/Cyanine5 anti-mouse I-A/I-E M5/114.15.2 FC
APC anti-mouse I-A/I-E M5/114.15.2 FC
Alexa Fluor® 488 anti-mouse I-A/I-E M5/114.15.2 FC,3D IHC
Alexa Fluor® 647 anti-mouse I-A/I-E M5/114.15.2 FC
Pacific Blue™ anti-mouse I-A/I-E M5/114.15.2 FC
Alexa Fluor® 700 anti-mouse I-A/I-E M5/114.15.2 FC,SB
PerCP/Cyanine5.5 anti-mouse I-A/I-E M5/114.15.2 FC
PerCP anti-mouse I-A/I-E M5/114.15.2 FC
APC/Cyanine7 anti-mouse I-A/I-E M5/114.15.2 FC
PE/Cyanine7 anti-mouse I-A/I-E M5/114.15.2 FC
Brilliant Violet 421™ anti-mouse I-A/I-E M5/114.15.2 FC,IHC-F,SB
Brilliant Violet 510™ anti-mouse I-A/I-E M5/114.15.2 FC
Purified anti-mouse I-A/I-E (Maxpar® Ready) M5/114.15.2 FC,CyTOF®
Brilliant Violet 605™ anti-mouse I-A/I-E M5/114.15.2 FC
Brilliant Violet 650™ anti-mouse I-A/I-E M5/114.15.2 FC
Brilliant Violet 711™ anti-mouse I-A/I-E M5/114.15.2 FC
Brilliant Violet 785™ anti-mouse I-A/I-E M5/114.15.2 FC
PE/Dazzle™ 594 anti-mouse I-A/I-E M5/114.15.2 FC
Alexa Fluor® 594 anti-mouse I-A/I-E M5/114.15.2 IHC-F,3D IHC
APC/Fire™ 750 anti-mouse I-A/I-E M5/114.15.2 FC
TotalSeq™-A0117 anti-mouse I-A/I-E M5/114.15.2 PG
Ultra-LEAF™ Purified anti-mouse I-A/I-E M5/114.15.2 FC,IHC-F,IP,Block
TotalSeq™-B0117 anti-mouse I-A/I-E M5/114.15.2 PG
TotalSeq™-C0117 anti-mouse I-A/I-E M5/114.15.2 PG
Spark Blue™ 550 anti-mouse I-A/I-E M5/114.15.2 FC
PE/Fire™ 640 anti-mouse I-A/I-E M5/114.15.2 FC
Spark YG™ 581 anti-mouse I-A/I-E M5/114.15.2 FC
PE/Fire™ 810 anti-mouse I-A/I-E M5/114.15.2 FC
Spark UV™ 387 anti-mouse I-A/I-E M5/114.15.2 FC
Spark Violet™ 538 anti-mouse I-A/I-E M5/114.15.2 FC
PerCP/Fire™ 806 anti-mouse I-A/I-E M5/114.15.2 FC
Spark Red™ 718 anti-mouse I-A/I-E M5/114.15.2 FC
APC/Fire™ 810 anti-mouse I-A/I-E M5/114.15.2 FC
Spark PLUS UV395™ anti-mouse I-A/I-E M5/114.15.2 FC
Go To Top Version: 4    Revision Date: 10.30.2014

For Research Use Only. Not for diagnostic or therapeutic use.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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