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PE anti-human LT-α (TNF-β) Antibody

Pricing & Availability
Clone
359-81-11 (See other available formats)
Regulatory Status
RUO
Other Names
Lymphotoxin-α (LT- α), Tumor necrosis factor-β (TNF-β), Coley's toxin, Hemorrhagic factor, Necrosin, Natural killer cytotoxic factor (NKCF), Differentiation inducing factor (DIF), TNFSF-1
Isotype
Mouse IgG1, κ
Ave. Rating
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Product Citations
publications
359-81-11_PE_LT-a_Antibody_1_091824
PMA+ionomycin-stimulated human T cells were surface stained with CD3 PE/Cyanine5 and intracellular stained with 359-81-11 PE
  • 359-81-11_PE_LT-a_Antibody_1_091824
    PMA+ionomycin-stimulated human T cells were surface stained with CD3 PE/Cyanine5 and intracellular stained with 359-81-11 PE
See PE spectral data
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503105 25 µg 129 CHF
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Description

Lymphotoxin-α (LT-α), also known as tumor necrosis factor-beta (TNF-β), is a potent lymphoid factor that exerts cytotoxic effects on a wide range of tumor cells and certain other target cells. LT-α possesses a signal peptide sequence and is a secreted protein; however, LT-α is also present on the surface of activated T, B and LAK cells as a complex with LT-β. Bioactive LT-α exists as a homotrimer.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
E. coli expressed, recombinant human LT-α.
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography, and conjugated with PE under optimal conditions.
Concentration
0.2 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

ICFC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by intracellular immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 µg per 106 cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Excitation Laser
Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Application Notes

ELISA or ELISPOT Detection1,2: The biotinylated 359-81-11 antibody is useful as a detection antibody for a sandwich ELISA or ELISPOT assay, when used in conjunction with purified 359-238-8 antibody (Cat. No. 503002/503004) as the capture antibody.
Flow Cytometry3: The fluorochrome-labeled 359-81-11 antibody is useful for intracellular immunofluorescent staining and flow cytometric analysis to identify LT-α -producing cells within mixed cell populations. View intracellular cytokine staining protocol.
Neutralization1,2: The 359-81-11 antibody can neutralize the bioactivity of natural or recombinant LT-α. The LEAF™ purified antibody (Endotoxin <0.1 EU/μg, Azide-Free, 0.2 μm filtered) is recommended for neutralization of human LT-α bioactivity (Cat. No. 503108).
Additional reported applications (for the relevant formats) include: immunohistochemical staining of paraformaldehyde-fixed, saponin-treated frozen tissue sections, and immunocytochemistry.

Application References

(PubMed link indicates BioLegend citation)
  1. Meager A, et al. 1987. J. Immunol. Methods 104:31.
  2. Meager A, et al. 1987. Hybridoma. 6:305.
  3. Jason J, et al. 1999. Clin. Diagn. Lab Immunol. 6:73.
RRID
AB_315275 (BioLegend Cat. No. 503105)

Antigen Details

Structure
TNF superfamily; trimer; 25 kD (Mammalian).
Function
Transformed cell cytotoxicity; mediator of inflammatory and immune functions; fibroblast synthesis of GM-CSF, G-CSF, IL-1, collagenase, prostaglandin E2; monocyte terminal differentiation, synthesis of G-CSF; neutrophil chemoattractant, production of reactive oxygen, fibroblast proliferation.
Cell Sources
Activated T and B cells, fibroblasts, astrocytes, myeloma, endothelial cells, epithelial cells.
Cell Targets
Monocytes, B cells, fibroblasts, neutrophils, osteoclasts, keratinocytes, endothelial cells.
Receptors
TNFRSF1A (TNF-R1, CD120a, TNFR-p60 Type β, p55); TNFRSF1B (TNF-R2, CD120b, TNFR-p80 Type A, p75).
Biology Area
Cell Biology, Immunology, Innate Immunity, Neuroinflammation, Neuroscience
Molecular Family
Cytokines/Chemokines
Antigen References

1. Fitzgerald, K., et al. Eds. 2001. The Cytokine FactsBook. Academic Press, San Diego.
2. Aggarwal, B., et al.Eds. 1992. Tumor necrosis factors:structure, function, and mechanism of action. Marcel Dekker Inc.
3. Bonavida, B., et al.Eds. 1990. Tumor necrosis factor:structure, mechanisms of action, role in disease and therapy. Karger, Basel.
4. Paul, N., et al. 1987. Annu. Rev. Immunol. 6:407.

Regulation
Type II integral membrane protein, forms heterotrimer with type II integral membrane protein LT-β either as LTα1β2 or LTα2β1; processed secreted form is trimeric.
Gene ID
4049 View all products for this Gene ID
Specificity (DOES NOT SHOW ON TDS):
LT-alpha
Specificity Alt (DOES NOT SHOW ON TDS):
LT-α
App Abbreviation (DOES NOT SHOW ON TDS):
ICFC
UniProt
View information about LT-alpha on UniProt.org

Related FAQs

What type of PE do you use in your conjugates?
We use R-PE in our conjugates.

Other Formats

View All LT-α Reagents Request Custom Conjugation
Description Clone Applications
Biotin anti-human LT-α (TNF-β) 359-81-11 ELISA Detection,ELISPOT Detection,ICFC
PE anti-human LT-α (TNF-β) 359-81-11 ICFC
Go To Top Version: 1    Revision Date: 11.30.2012

For Research Use Only. Not for diagnostic or therapeutic use.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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