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- Clone
- W16012C (See other available formats)
- Regulatory Status
- RUO
- Other Names
- bHLHe76, aryl hydrocarbon receptor, Aryl hydrocarbon receptor, RP85
- Isotype
- Rat IgG2b, κ
- Ave. Rating
- Submit a Review
- Product Citations
- publications
-
C57BL/6 mouse splenocytes were stimulated with (A) or without (B) recombinant human TGF-β1 and recombinant mouse IL-6 for 3 days. Cells were surface stained with anti-mouse CD4 APC, fixed and permeabilized, then intracellularly stained with anti-mouse AhR PE (clone W16012C).
| Cat # | Size | Price | Quantity Check Availability | Save | ||
|---|---|---|---|---|---|---|
| 162703 | 25 µg | 214 CHF | ||||
| 162704 | 100 µg | 528 CHF | ||||
Aryl Hydrocarbon Receptor or AhR is a bHLH transcription factor that is activated by ligands such as TCDD. It is expressed in many immune cells and perform an important role in immune regulation. AhR is induced under TH17-polarizing conditions. In its inactive state, AhR presents in the cytoplasm, and upon ligand binding it translocates to the nucleus. Activation of AhR is tightly controlled by Ubiquitination, ligand metabolism, and complex disruption.
Product DetailsProduct Details
- Verified Reactivity
- Mouse
- Antibody Type
- Monoclonal
- Host Species
- Rat
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
- Preparation
- The antibody was purified by affinity chromatography and conjugated with PE under optimal conditions.
- Concentration
- 0.2 mg/mL
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
- Application
-
ICFC - Quality tested
- Recommended Usage
-
Each lot of this antibody is quality control tested by intracellular immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.5 µg per million cells in 100 µL volume. It is recommended that the reagent be titrated for optimal performance for each application.
- Excitation Laser
-
Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
- RRID
-
AB_2892335 (BioLegend Cat. No. 162703)
Antigen Details
- Gene ID
- 11622 View all products for this Gene ID
- Specificity (DOES NOT SHOW ON TDS):
- AhR
- Specificity Alt (DOES NOT SHOW ON TDS):
- AhR
- App Abbreviation (DOES NOT SHOW ON TDS):
- ICFC
- UniProt
- View information about AhR on UniProt.org
Related FAQs
- What type of PE do you use in your conjugates?
- We use R-PE in our conjugates.
Other Formats
View All AhR Reagents Request Custom Conjugation| Description | Clone | Applications |
|---|---|---|
| PE anti-mouse AhR Antibody | W16012C | ICFC |
| Purified anti-mouse AhR Antibody | W16012C | ICFC |
Customers Also Purchased
Compare Data Across All Formats
This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.
-
PE anti-mouse AhR Antibody
C57BL/6 mouse splenocytes were stimulated with (A) or withou... -
Purified anti-mouse AhR Antibody
C57BL/6 mouse splenocytes were cultured for 3 days with (fil...
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