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PE/Cyanine7 anti-mouse CD24 Antibody

Pricing & Availability
Clone
M1/69 (See other available formats)
Regulatory Status
RUO
Other Names
Heat Stable Antigen (HSA), Ly-52, Nectadrin
Isotype
Rat IgG2b, κ
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Product Citations
publications
M1slash69_PECy7_080207
C57BL/6 mouse splenocytes stained with M1/69 PE/Cyanine7
  • M1slash69_PECy7_080207
    C57BL/6 mouse splenocytes stained with M1/69 PE/Cyanine7
Compare all formats See PE/Cyanine7 spectral data
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101821 25 µg 101 CHF
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101822 100 µg 270 CHF
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Description

CD24 is a 35-45 kD protein also known as Heat Stable Antigen (HSA), Ly-52, or Nectadrin. It is a GPI-linked sialoglycoprotein expressed on lymphocytes, granulocytes, epithelial cells, thymocytes, monocytes, erythrocytes, and dendritic cells. CD24 expression varies during T and B cell differentiation and is a useful marker for delineating various lymphocyte developmental stages. CD24 serves as an adhesion or costimulatory molecule involved in T and B lymphocyte activation and differentiation by homophilic binding or binding to CD62P.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
C57BL/10 mouse splenic T cells and concanavalin A-activated splenocytes
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography, and conjugated with PE/Cyanine7 under optimal conditions.
Concentration
0.2 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 µg per 106 cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Excitation Laser
Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Application Notes

Additional reported applications (for the relevant formats) include: Western blotting1, in vitro induction of thymocyte maturation2, complement-mediated cytotoxicity3, and immunohistochemistry of acetone-fixed frozen sections4, formalin-fixed paraffin-embedded sections5 and zinc-fixed paraffin-embedded sections10. The Ultra-LEAF™ purified antibody (Endotoxin < 0.01 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (Cat. No. 101845 and 101846).

Application References

(PubMed link indicates BioLegend citation)
  1. Springer T, et al. 1978. Eur. J. Immunol. 8:539. (WB)
  2. Crowley M, et al. 1989. Cell. Immunol. 118:108. (FA)
  3. Veillette A, et al. 1989. J. Exp. Med. 170:1671. (FA)
  4. Pandelakis A Flavell RA 1999 JEM 189:855 (FC, IHC)
  5. Liu JQ, et al. 2007 J. Immunol. 178:6227. (FC, IF)
  6. Chappaz S, et al. 2007. Blood doi:10.1182/blood-2007-02-074245. (FC) PubMed
  7. Rucci F, et al. 2010. Proc Natl Acad Sci USA. 107:3024. (FC) PubMed
  8. Teague TK, et al. 2010. Int Immunol. 22:387. (FC) PubMed
  9. Gracz AD, et al. 2010. Am J. Physiol Gastrointest Liver Physiol. 298:590. (FC) PubMed
  10. Chen CY, et al. 2008. Endocrinology. 10:1210. (FC, IHC) PubMed
  11. Qui Q, et al. 2010. J. Immunol. 184:1681. (FC) PubMed
Product Citations
  1. Song W, et al. 2019. Cell Res. 29:206. PubMed
  2. Wu Z, et al. 2023. EMBO Rep. 24:e56524. PubMed
  3. Zhou Y, et al. 2023. NPJ Regen Med. 8:23. PubMed
  4. Gargaro M, et al. 2022. Immunity. 55:1032. PubMed
  5. Schneider C, et al. 2018. Cell. 174:271. PubMed
  6. Kim S, et al. 2020. Immunity. 53(4):759-774.e9. PubMed
  7. Barry KC, et al. 2018. Nat Med. 24:1178. PubMed
  8. Qiu Q, et al. 2010. J Immunol. 184:1681. PubMed
  9. Piper CJM, et al. 2020. Cell Reports. 29(7):1878-1892.e7.. PubMed
  10. Geng A, et al. 2020. eLife. 9:e56434.. PubMed
  11. Liu X, et al. 2021. eLife. 0.416666666666667. PubMed
  12. Privratsky JR, et al. 2018. Am J Physiol Renal Physiol. 315:F682. PubMed
  13. Huang WJ, et al. 2021. Theranostics. 11:5539. PubMed
  14. Lafkas D, et al. 2013. J Cell Biol. 203:47. PubMed
  15. Yang L, et al. 2021. Cell Death Differ. 28:2616. PubMed
  16. Ruhland MK, et al. 2020. Cancer Cell. 37(6):786-799.e5. PubMed
  17. Kass E, et al. 2016. Nat Commun. 7:13241. PubMed
  18. Codina A, et al. 2019. Cell Syst. 8:136. PubMed
  19. Zhu Y, et al. 2022. Clin Transl Med. 12:e887. PubMed
  20. Wang J, et al. 2021. Cell Reports. 34(13):108897. PubMed
  21. Park JY, et al. 2019. Cell Rep. 27:2548. PubMed
  22. Brandi P, et al. 2022. Cell Rep. 38:110184. PubMed
  23. Wang R, et al. 2021. Front Cell Dev Biol. 9:772669. PubMed
  24. Zhou Q, et al. 2014. J Immunol. 193:496. PubMed
  25. Lu Y, et al. 2018. Stem Cells. 36:1875. PubMed
  26. Cai C, et al. 2019. Developmental Biology. 458(1):43-51. PubMed
  27. Königsberger S, et al. 2010. PLoS One. 5:e12468. PubMed
  28. Cui Z, et al. 2021. Cell Death Dis. 12:775. PubMed
  29. Rosser EC, et al. 2020. Cell Metabolism. 31(4):837-851. PubMed
  30. O'Leary CE, et al. 2021. Curr Protoc. 1:e77. PubMed
  31. Clemente–Casares X, et al. 2017. Immunity. 47:974. PubMed
  32. Di Lorenzo A, et al. 2022. Oncoimmunology. 11:2086752. PubMed
  33. Kiss M, et al. 2020. Cancer Immunol Res. 9:309. PubMed
  34. Bird T, et al. 2013. Proc Natl Acad Sci U S A. 110:6542. PubMed
  35. Mujal AM, et al. 2022. Cancer Immunol Res. 10:403. PubMed
  36. Daynac M, et al. 2016. Stem Cell Reports. 7:735-748. PubMed
  37. Andrés B, et al. 2012. J Immunol. 189:2300. PubMed
  38. Jing Y, et al. 2019. J Allergy Clin Immunol. 144:1377. PubMed
  39. Shue YT, et al. 2022. Nat Commun. 13:2690. PubMed
  40. Kojima T, et al. 2016. Sci Rep. 6:36457. PubMed
  41. Zhou C, et al. 2020. Gut Microbes. 12:1782156. PubMed
  42. Huang X, et al. 2021. Immunity. . PubMed
  43. Sfakianos JP, et al. 2020. Nat Commun. 2.222222222. PubMed
  44. Seong J, et al. 2018. Development. 145:14. PubMed
  45. Du Z, et al. 2020. J Allergy Clin Immunol. . PubMed
  46. Avgustinova A, et al. 2021. Cell Stem Cell. . PubMed
RRID
AB_756047 (BioLegend Cat. No. 101821)
AB_756047 (BioLegend Cat. No. 101822)

Antigen Details

Structure
GPI-linked sialoglycoprotein, 35-45 kD
Distribution

B cells, granulyocytes, epithelial cells, thymocytes, monocytes, erythrocytes, dendritic cells

Function
Regulates B cell proliferation and differentiation
Ligand/Receptor
P-selectin, CD24
Cell Type
B cells, Dendritic cells, Epithelial cells, Erythrocytes, Granulocytes, Monocytes, Thymocytes
Biology Area
Immunology
Molecular Family
CD Molecules
Antigen References

1. Barclay A, et al. 1997. The Leukocyte Antigen FactsBook Academic Press.
2. Aigner S, et al. 1997. Blood 89:3385.
3. Hough MR, et al. 1996. J. Immunol. 156:479.
4. Liu Y, et al. 1992. J. Exp. Med. 175:437.

Gene ID
12484 View all products for this Gene ID
Specificity (DOES NOT SHOW ON TDS):
CD24
Specificity Alt (DOES NOT SHOW ON TDS):
CD24
App Abbreviation (DOES NOT SHOW ON TDS):
FC
UniProt
View information about CD24 on UniProt.org
Go To Top Version: 1    Revision Date: 11.30.2012

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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