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PE/Cyanine7 anti-mouse CD279 (PD-1) Antibody

Pricing & Availability
Clone
RMP1-30 (See other available formats)
Regulatory Status
RUO
Other Names
Programmed Death-1, CD279, PDCD1
Isotype
Rat IgG2b, κ
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Product Citations
publications
RMP1-30_PECy7_021808
Con A-stimulated (day-3) Balb/c mouse splenocytes stained with RMP1-30 PE/Cyanine7
  • RMP1-30_PECy7_021808
    Con A-stimulated (day-3) Balb/c mouse splenocytes stained with RMP1-30 PE/Cyanine7
Compare all formats See PE/Cyanine7 spectral data
Cat # Size Price Quantity Check Availability Save
109109 25 µg 110 CHF
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109110 100 µg 292 CHF
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Description

CD279 is a 50-55 kD immunoglobulin superfamily member also known as programmed death-1 (PD-1). PD-1 is expressed on a subset of CD4-CD8- thymocytes and on activated T and B cells. PD-1 is thought to be involved in lymphocyte clonal selection and peripheral tolerance. The PD-1 ligands, PD-L1 (also known as B7-H1) and PD-L2 (B7-DC), are members of the B7 immunoglobulin superfamily.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Mouse PD-1 transfected BHK cells
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography, and conjugated with PE/Cyanine7 under optimal conditions.
Concentration
0.2 mg/ml
Storage & Handling
The CD279 antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is =1.0 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Excitation Laser
Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Application Notes

The RMP1-30 antibody does not block the binding of PD-1 to B7-H1 and B7-DC1.

Application References

(PubMed link indicates BioLegend citation)
  1. Matsumoto K, et al. 2004. J. Immunol. 172:2530.
  2. Raimondi G, et al. 2006. J. Immunol. 176:2808. (FC) PubMed
  3. King IL, et al. 2009. J. Exp Med 206:1001. (FC) PubMed
Product Citations
  1. Lindenstrøm T, et al. 2013. J Immunol. 190:6311. PubMed
  2. Ozga AJ, et al. 2022. Immunity. 55:82. PubMed
  3. Chakraborty P, et al. 2022. Cancer Res. 82:1969. PubMed
  4. Grebinoski S, et al. 2022. Nat Immunol. 23:868. PubMed
  5. Balood M, et al. 2022. Nature. 611:405. PubMed
  6. Zhao K, et al. 2023. Front Immunol. 14:1101769. PubMed
  7. Zhang W, et al. 2022. Dev Cell. 57:329. PubMed
  8. Zander R, et al. 2022. Immunity. 55:475. PubMed
  9. Belk JA, et al. 2022. Cancer Cell. 40:768. PubMed
  10. Duhalde Vega M, et al. 2022. Sci Adv. 8:eabn6545. PubMed
  11. Zander R, et al. 2022. Elife. 11:. PubMed
  12. Ke F, et al. 2023. Elife. 12:. PubMed
  13. Liu KY, et al. 2023. Environ Health Perspect. 131:37014. PubMed
  14. Gern BH, et al. 2021. Cell Host Microbe. 29(4):594-606.e6. PubMed
  15. Vanderleyden I, et al. 2020. Cell Rep. 30:611. PubMed
  16. Singer M et al. 2016. Cell. 166(6):1500-1511 . PubMed
  17. Turner JS et al. 2018. Cell reports. 25(6):1395-1403 . PubMed
  18. Blecher-Gonen R, et al. 2019. Cell Syst. 8:109. PubMed
  19. Webb LMC, et al. 2021. Aging Cell. 20:e13295. PubMed
  20. Andrade MS, et al. 2022. JCI Insight. 7:. PubMed
  21. Wu M, et al. 2021. Nat Commun. 12:3500. PubMed
  22. Low MSY, et al. 2019. Cell Reports. 29(9):2634-2645.e5. PubMed
  23. McLane LM, et al. 2021. Cell Reports. 35(6):109120. PubMed
  24. Acharya N, et al. 2020. Immunity. 53(3):658-671.e6. PubMed
  25. Shi J, et al. 2018. Immunity. 49:264. PubMed
  26. Pérez‐Mazliah D et al. 2017. EBioMedicine. 24:216-230 . PubMed
  27. Elsner RA, et al. 2020. Cell Reports. 29(9):2796-2809.e5.. PubMed
  28. Mohrs I 2009. J Exp Med. 206:1001. PubMed
  29. Montes de Oca R, et al. 2021. Mol Cancer Ther. 20:1941. PubMed
  30. Chen JS, et al. 2022. Sci Immunol. 7:eabl5652. PubMed
  31. Dangi T, et al. 2020. Curr Protoc Immunol. 130:e99. PubMed
  32. Suah AN, et al. 2021. J Clin Invest. 131:. PubMed
  33. Yu AI, et al. 2020. Cell Rep. 107471:31. PubMed
  34. Biram A, et al. 2020. Cell Rep. 30:1910. PubMed
  35. Hilpert C, et al. 2016. J Immunol. 197: 2780 - 2786. PubMed
  36. Panciera T, et al. 2016. Cell Stem Cell. 19:725-737. PubMed
  37. Stump CT, et al. 2021. Open Biol. 11:210245. PubMed
  38. Jeon S, et al. 2013. J Immunol. 190:6277. PubMed
  39. Siddiqui I et al. 2019. Immunity. 50(1):195-211 . PubMed
  40. Hossain DMS, et al. 2018. J Clin Invest. 128:644. PubMed
  41. Verghese DA, et al. 2018. JCI Insight. 3:. PubMed
  42. Chen Z et al. 2019. Immunity. 51(5):840-855 . PubMed
  43. Myers DR et al. 2019. Cell reports. 27(6):1858-1874 . PubMed
  44. Wang Y, et al. 2021. Cell Rep. 35:108948. PubMed
  45. , et al. 2021. Eur J Immunol. 51:2708. PubMed
  46. Fu G, et al. 2021. Nature. 595:724. PubMed
  47. Gary E, et al. 2020. Vaccine. 3821:38. PubMed
  48. Volberding PJ, et al. 2021. Cell Reports. 35(8):109160. PubMed
  49. Ringel AE, et al. 2020. Cell. 183(7):1848-1866.e26. PubMed
  50. Sullivan JA, et al. 2020. Cell Rep. 30:1039. PubMed
  51. Jing Y, et al. 2020. Sci Adv. 6:eaax9455. PubMed
  52. Yadava K et al. 2019. Elife. 8 pii: e44821. PubMed
  53. Van Gool F, et al. 2019. Immunity. 50:362. PubMed
  54. Jain RW, et al. 2018. Cell Rep. 25:3342. PubMed
  55. Trefzer A, et al. 2021. Cell Reports. 34(6):108748. PubMed
  56. Gabriel SS, et al. 2021. Immunity. 54(8):1698-1714.e5. PubMed
  57. Alameh MG, et al. 2021. Immunity. 54:2877. PubMed
  58. Frost JN, et al. 2021. Med (N Y). 2:164. PubMed
  59. Zotos D, et al. 2021. Nat Commun. 12:7160. PubMed
  60. Biram A, et al. 2020. Bio Protoc. e3562:10. PubMed
  61. Vardhana SA, et al. 2020. Nat Immunol. 1.584722222. PubMed
  62. Jin C, et al. 2019. Cell. 176:998. PubMed
  63. Ying Zhang et al. 2017. Cancer cell. 32(3):377-391 . PubMed
  64. Hilpert C, et al. 2019. J Immunol. 203:3068. PubMed
  65. Chatterjee S et al. 2017. Cell metabolism. 27(1):85-100 . PubMed
  66. Etzerodt A, et al. 2019. J Exp Med. 216:2394. PubMed
  67. Xiao S, et al. 2020. Cell Reports. 32(2):107892. PubMed
  68. Evgin L, et al. 2020. Nat Commun. 2.671527778. PubMed
  69. Wu S, et al. 2021. Nat Cancer. 2:189. PubMed
  70. Benci JL et al. 2019. Cell. 178(4):933-948 . PubMed
  71. Woodworth J, et al. 2014. J Immunol. 192:3247. PubMed
  72. Miyauchi K, et al. 2016. Nat Immunol. 17:1447-1458. PubMed
  73. Chen Y, et al. 2021. Nat Immunol. 22:996. PubMed
  74. Miyauchi K, et al. 2021. Nat Commun. 12:3789. PubMed
  75. Chen Z, et al. 2021. Cell. 184(5):1262-1280.e22. PubMed
  76. Benci JL et al. 2016. Cell. 167(6):1540-1554 . PubMed
  77. Sema Kurtulus et al. 2018. Immunity. 50(1):181-194 . PubMed
  78. Fan MY et al. 2018. Cell reports. 25(5):1204-1213 . PubMed
  79. Filskov J, et al. 2019. Sci Rep. 9:14085. PubMed
  80. McPhee C, et al. 2013. J Immunol. 191:4581. PubMed
  81. Burger ML, et al. 2021. Cell. 184:4996. PubMed
  82. Liu D, et al. 2021. Immunity. 54:2245. PubMed
  83. Smith LK, et al. 2021. Elife. 10:. PubMed
  84. Xu C, et al. 2021. Cell Reports. 35(11):109235. PubMed
  85. Dubrot J, et al. 2021. Immunity. 54(3):571-585.e6. PubMed
  86. Lederer K, et al. 2020. Immunity. 53(6):1281-1295.e5. PubMed
  87. Song W, et al. 2022. Immunity. 55:290. PubMed
  88. Christensen D, et al. 2016. Mucosal Immunol. 10.1038/mi.2016.28. PubMed
  89. Zander R, et al. 2020. Immunity. 51(6):1028-1042.e4.. PubMed
  90. Nawaf M, et al. 2017. J Immunol. 10.4049/jimmunol.1700088. PubMed
RRID
AB_572016 (BioLegend Cat. No. 109109)
AB_572016 (BioLegend Cat. No. 109110)

Antigen Details

Structure
Ig superfamily, 50-55 kD
Distribution

Subset of double negative thymocytes, activated T and B cells

Function
Lymphocyte clonal selection, peripheral tolerance
Ligand/Receptor
PD-L1 (B7-H1), PD-L2
Cell Type
B cells, T cells, Thymocytes, Tregs
Biology Area
Apoptosis/Tumor Suppressors/Cell Death, Cancer Biomarkers, Cell Biology, Immunology, Inhibitory Molecules
Molecular Family
CD Molecules, Immune Checkpoint Receptors
Antigen References

1. Barclay A, et al. 1997. The Leukocyte Antigen FactsBook Academic Press.
2. Agata Y, et al. 1996. Int. Immunol. 8:765.
3. Nishimura H, et al. 2001. Science 291:319.
4. Ishida Y, et al. 1992. EMBO J. 11:3887.

Gene ID
18566 View all products for this Gene ID
Specificity (DOES NOT SHOW ON TDS):
CD279
Specificity Alt (DOES NOT SHOW ON TDS):
CD279
App Abbreviation (DOES NOT SHOW ON TDS):
FC
UniProt
View information about CD279 on UniProt.org
Go To Top Version: 1    Revision Date: 11.30.2012

For Research Use Only. Not for diagnostic or therapeutic use.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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