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Purified anti-human CD117 (c-kit) Antibody

Pricing & Availability
Clone
W18195C (See other available formats)
Regulatory Status
RUO
Other Names
Stem cell factor receptor, c-kit, mast cell growth factor receptor, steel factor receptor
Isotype
Rat IgG2a, κ
Ave. Rating
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Product Citations
publications
W18195C_PURE_CD117_Antibody_070720.png
Kasumi-3 cells (an acute myeloblastic leukemia cell line) were stained with purified anti-human CD117 (c-kit) (clone W18195C) (closed histogram) or purified rat IgG2a, κ isotype control (open histogram), followed by goat anti-rat IgG PE.
  • W18195C_PURE_CD117_Antibody_070720.png
    Kasumi-3 cells (an acute myeloblastic leukemia cell line) were stained with purified anti-human CD117 (c-kit) (clone W18195C) (closed histogram) or purified rat IgG2a, κ isotype control (open histogram), followed by goat anti-rat IgG PE.
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375302 100 µg 176 CHF
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Description

CD117 is a 145 kD protein tyrosine kinase also known as c-kit. It is a receptor for stem cell factor or c-kit ligand. CD117 is expressed on pluripotent hematopoietic progenitor cells (approximately 1-4% bone marrow cells), mast cells, and acute myeloid leukemia cells (AML). CD117 binding of c-kit ligand induces phosphorylation of CD117 and stimulates proliferation and survival of primitive hematopoietic stem cells as well as erythroid-committed and granulo-monocytic committed cells.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Human CD117 transfectant
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/mL
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.5 µg per million cells in 100 µL volume. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

Clone W18195C antibody does not block the binding of clones 104D2 or S18022G to the target. Clone W18195C antibody does not bind to the target after cells are fixed by 4% PFA.

RRID
AB_2890812 (BioLegend Cat. No. 375302)

Antigen Details

Structure
Growth factor receptor with tyrosine kinase activity, subclass III, approximately 145 kD
Distribution

Pluripotent hematopoietic progenitor cells (approximately 1-4% bone marrow cells), mast cells, acute myeloid leukemic cells (AML)

Function
Receptor for stem cell factor. Induces proliferation and survival of primitive hematopoietic progenitors. Potent inducer of proliferation in erythroid-committed progenitor cells. Defects in CD117 have been linked to severe anemia and a decreased number of hematopoietic progenitor cells.
Ligand/Receptor
Stem cell factor (c-kit ligand)
Cell Type
Embryonic Stem Cells, Hematopoietic stem and progenitors, Leukemia, Mast cells, Mesenchymal Stem Cells
Biology Area
Immunology, Stem Cells
Molecular Family
CD Molecules
Antigen References

1. Giebel LB, et al. 1992. Oncogene. 7:2207.
2. Furitsu T, et al. 1993. J. Clin. Invest. 92:1736.

Gene ID
3815 View all products for this Gene ID
Specificity (DOES NOT SHOW ON TDS):
CD117
Specificity Alt (DOES NOT SHOW ON TDS):
CD117
App Abbreviation (DOES NOT SHOW ON TDS):
FC
UniProt
View information about CD117 on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 2    Revision Date: 07.08.2020

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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