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Purified anti-human CD166 Antibody

Pricing & Availability
Clone
3A6 (See other available formats)
Regulatory Status
RUO
Workshop
HCDM listed
Other Names
CD6 ligand, Activated Leukocyte Cell Adhesion Molecule (ALCAM)
Isotype
Mouse IgG1, κ
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Product Citations
publications
A_3A6_PE_082509
PHA-stimulated human peripheral blood lymphocytes (3 days) stained with purified 3A6 conjugated to PE
  • A_3A6_PE_082509
    PHA-stimulated human peripheral blood lymphocytes (3 days) stained with purified 3A6 conjugated to PE
  • B_3A6_PE_082509
    Human peripheral blood monocytes stained with purified 3A6 conjugated to PE
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343902 100 µg 130 CHF
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Description

CD166, also known as the CD6 ligand or the Activated Leukocyte Cell Adhesion Molecule (ALCAM), is a 100-105 kD transmembrane glycoprotein. It belongs to the Ig superfamily of proteins and expressed on activated T cells, activated monocytes, epithelial cells, fibroblasts, and neurons. CD166 plays an important role in mediating adhesion interactions between thymic epithelial cells and CD6+ cells during intrathymic T cell development. Recently CD166 has also been used as a potential cancer stem cell marker. The antibody reacts with human activated leukocyte cell adhesion molecule (ALCAM).

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human
Reported Reactivity
African Green, Baboon, Cynomolgus, Rhesus
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Cultured human thymic epithelial cells
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

FC - Quality tested
IHC-P, ICC - Reported in the literature, not verified in house
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.5 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

Additional reported applications (for the relevant formats) include: immunohistochemical staining of paraffin-embedded tissue sections and immunofluorescence.1

Application References

(PubMed link indicates BioLegend citation)
  1. Pretzel D, et al. 2011. Arthritis Res. Ther. 13:R64. (IHC, IF, FC)
Product Citations
  1. Slamecka J, et al. 2017. Cell Cycle. 17:330. PubMed
  2. Müller M, et al. 2021. Nat Commun. 12:7036. PubMed
  3. Blázquez-Prunera A, et al. 2017. Stem Cell Res Ther. 10.1186/s13287-017-0552-z. PubMed
  4. Dutertre CA, et al. 2020. Immunity. 51(3):573-589.e8.. PubMed
RRID
AB_2223892 (BioLegend Cat. No. 343902)

Antigen Details

Structure
A type I transmembrane glycoprotein belonging to the Ig superfamily of proteins.
Distribution

CD166 is expressed on activated T cells, activated monocytes, epithelial cells, fibroblasts, and neurons.

Function
Play an important role in mediating adhesion interactions between thymic epithelial cells and CD6+ cells during intrathymic T cell development.
Ligand/Receptor
CD6
Cell Type
Epithelial cells, Fibroblasts, Mesenchymal Stem Cells, Monocytes, Neurons, T cells
Biology Area
Immunology, Stem Cells
Molecular Family
Adhesion Molecules, CD Molecules
Antigen References

1. Aruffo A, et al. 1997. Immunol Today. 18(10):498
2. Patel DD, et al. 1995. J. Exp. Med. 181:2213
3. Bowen MA, et al. 1995. J. Exp. Med. 181:1563
4. Horst D, et al. 2009. Cancer Invest. 22:1

Gene ID
214 View all products for this Gene ID
Specificity (DOES NOT SHOW ON TDS):
CD166
Specificity Alt (DOES NOT SHOW ON TDS):
CD166
App Abbreviation (DOES NOT SHOW ON TDS):
FC,IHC-P,ICC
UniProt
View information about CD166 on UniProt.org
Go To Top Version: 4    Revision Date: 08.18.2022

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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