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Purified anti-human CD66a/c/e Antibody

Pricing & Availability
Clone
ASL-32 (See other available formats)
Regulatory Status
RUO
Other Names
CEACAM1/5/6, BGP, NCA
Isotype
Mouse IgG2b, κ
Ave. Rating
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Product Citations
publications
ASL-32_PE_051409.jpg
Human colorectal adenocarcinoma cell line HT29 stained with purified ASL-32 conjugated to PE
  • ASL-32_PE_051409.jpg
    Human colorectal adenocarcinoma cell line HT29 stained with purified ASL-32 conjugated to PE
  • ASL-32_PURE_CD66ace_Antibody_IHC-P_022719
    Human paraffin-embedded colon tissue slices were prepared with a standard protocol of deparaffination and rehydration. Antigen retrieval was done with Tris-Buffered Saline 1X (1.0M, pH7.4) at 95°C for 40 minutes. Tissue was washed with PBS/ 0.05% Tween20 twice for five minutes and blocked with 5% FBS and 0.2% gelatin for 30 minutes. Then, the tissue was stained with 10 µg/ml of purified anti-human CD66a/c/e (clone ASL-32) antibody overnight at 4°C. On the next day, the tissue was incubated with Alexa Fluor® 594 Goat anti-mouse IgG (clone Poly4053) antibody (red) for an hour. Nuclei were counterstained with DAPI (blue). The image was captured with a 10X objective.
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342302 100 µg 141 CHF
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Description

The ASL-32 mAb reacts with an antigen epitope shared by CD66a, c and e. CD66a/c/e are members of the CEA (carcinoembryonic antigen) family of the Ig superfamily. CD66 plays a role in hemophilic and heterophilic adhesion. CD66a, also known as CEACAM1 and BGP (biliary glycoprotein), is mainly expressed on granulocytes, binds to CD66a, c, e, and CD62E. CD66c (known as CEACAM6, NCA) is expressed on granulocytes and epithelial cells. The ligands of CD66c are CD66a-e, CD62E and Galectins. CD66e molecule is also called CEA or CEACAM5, and is primarily found on epithelial cells. CD66e binds to CD66a, c and e.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human
Antibody Type
Monoclonal
Host Species
Mouse
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

FC - Quality tested
IHC-P - Verified
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.125 µg per million cells in 100 µl volume. For immunohistochemical staining on formalin-fixed paraffin-embedded tissue sections, the suggested use of this reagent is 5.0 - 10 µg per ml. It is recommended that the reagent be titrated for optimal performance for each application.

Application References

(PubMed link indicates BioLegend citation)
  1. Shey MS, et al. 2012. J Immunol Methods. 376:79. PubMed.
Product Citations
  1. Galaski J, et al. 2021. Front Cell Infect Microbiol. 11:692544. PubMed
  2. Obiedat A, et al. 2020. J Mol Med (Berl). 98:135. PubMed
  3. Evrard M et al. 2018. Immunity. 48(2):364-379 . PubMed
  4. Chevrier S, et al. 2021. Cell Reports Medicine. 2(1):100166. PubMed
  5. Shey M, et al. 2012. J Immunol Methods. 376:79. PubMed
  6. Sander J et al. 2017. Immunity. 47(6):1051-1066 . PubMed
  7. Chevrier S, et al. 2018. Cell Syst. 0.675. PubMed
  8. Baguma R, et al. 2017. PLoS One. 10.1371/journal.pone.0184563. PubMed
RRID
AB_1626265 (BioLegend Cat. No. 342302)

Antigen Details

Structure
Member of the CEA (carcinoembryonic antigen) family of the Ig superfamily
Distribution

Granulocytes, epithelial cells

Function
Homophilic and heterophilic adhesion
Ligand/Receptor
CD66a-e, CD62E, galectins
Cell Type
Epithelial cells, Granulocytes
Biology Area
Immunology
Molecular Family
Adhesion Molecules, CD Molecules
Antigen References

1. Zola H, et al. 2007. Leukocyte and Stromal Cell Molecules:The CD Markers. Wiley-Liss. A John Wiley & Sons Inc. Publication

Gene ID
634 View all products for this Gene ID
Specificity (DOES NOT SHOW ON TDS):
CD66a CD66c CD66e
Specificity Alt (DOES NOT SHOW ON TDS):
CD66a/c/e
App Abbreviation (DOES NOT SHOW ON TDS):
FC,IHC-P
UniProt
View information about CD66a CD66c CD66e on UniProt.org
Go To Top Version: 2    Revision Date: 01.05.2017

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
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