- Clone
- SMI 94 (See other available formats)
- Regulatory Status
- RUO
- Other Names
- Golli-MBP, myelin A1 protein, myelin membrane encephalitogenic protein
- Isotype
- Mouse IgG1, κ
- Ave. Rating
- Submit a Review
- Product Citations
- publications
Cat # | Size | Price | Quantity Check Availability | Save | ||
---|---|---|---|---|---|---|
836504 | 100 µg | 276 CHF |
Myelin basic protein (MBP) is a protein involved in the myelination of nerves in the central nervous system (CNS). MBP functions to maintain the correct structure of myelin, through its interaction with the lipids in the myelin membrane. The myelin sheath is made up of MBP and lipids, and acts as an insulator to increase the velocity of axonal impulse conduction. MBP plays a central role in the demyelinating disease multiple sclerosis (MS). A hallmark of the disease is the loss of the myelin sheath surrounding nerves, thought to be induced by antibodies against MBP.
Product DetailsProduct Details
- Verified Reactivity
- Human, Mouse, Rat
- Antibody Type
- Monoclonal
- Host Species
- Mouse
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
- Preparation
- The antibody was purified by affinity chromatography.
- Concentration
- 0.5 mg/ml
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C.
- Application
-
IHC-P - Quality tested
WB - Verified
ICC, IHC-F - Reported in the literature, not verified in house - Recommended Usage
-
Each lot of this antibody is quality control tested by formalin-fixed paraffin-embedded immunohistochemcical staining. For immunohistochemistry, a concentration range of 1.0 - 5.0 μg/ml is suggested. For Western blotting, the suggested use of this reagent is 1.0 - 5.0 µg/ml. It is recommended that the reagent be titrated for optimal performance for each application.
- Application Notes
-
SMI 94 reacts with the Myelin Basic Protein (MBP) peptic fragment 70-89 of the classic human myelin basic protein sequence.
SMI 94 detects developing and adult myelin, developing oligodendrocytes and distinguishes oligodendrocytes from astrocytes, microglia, neurons and other cells in brain sections. The combination of SMI 94 with SMI 91 and/or 99 is useful for immunocytochemical studies on the progression of normal and pathologic myelination. -
Application References
(PubMed link indicates BioLegend citation) -
- Kiryu-Seo S, et al. 2010. J. Neurosci. 30:6658. (ICC)
- Gensel JC, et al. 2009. J. Neurosci. 29:3956. (IHC-P)
- Wang H, et al. 2008. J. Cell. Biol. 182:1171. (ICC, WB)
- McLean NA, et al. 2014. PLoS One 9:e110174. (IHC-F)
- Tian C, et al. 2014. Front Cell Neurosci. 8:297. (IHC-P)
- Wüthrich C, et al. 2009. J. Neuropathol. Exp. Neurol. 68:15. (IHC, ICC)
- Maire CL, et al. 2014. Stem Cells 32:313. (IHC-P)
- Rusielewicz T, et. al. 2014. Glia 62:580. (ICC)
- Li C, et al. 2012. ASN Neuro. 4:e00102. (ICC)
- Huang JH, et al. 2009. Tissue Eng. Part A 15:1677. (IHC-P)
- Göttle P, et al. 2015. J. Neurosci. 35:906. (ICC)
- Zhao X, et al. 2010. Neuron. 65:612. (ICC)
- Product Citations
-
- RRID
-
AB_2616694 (BioLegend Cat. No. 836504)
Antigen Details
- Structure
- Myelin Basic Protein is a 304 amino acid protein with a molecular mass of 33kD.
- Distribution
-
Cytoskeleton, plasma membrane, cytosol, and extracellular.
- Function
- Classic MBP isoforms (4-14) are the most abundant protein components of the myelin membrane in the CNS. They play a role in both its formation and stabilization. The non-classic isoforms may have a role in the early developing brain long before myelination.
- Biology Area
- Cell Biology, Neuroscience, Neuroscience Cell Markers
- Gene ID
- 4155 View all products for this Gene ID
- Specificity (DOES NOT SHOW ON TDS):
- Myelin Basic Protein
- Specificity Alt (DOES NOT SHOW ON TDS):
- Myelin Basic Protein
- App Abbreviation (DOES NOT SHOW ON TDS):
- IHC-P,WB,ICC,IHC-F
- UniProt
- View information about Myelin Basic Protein on UniProt.org
Related Pages & Pathways
Pages
Related FAQs
Other Formats
View All Myelin Basic Protein Reagents Request Custom ConjugationDescription | Clone | Applications |
---|---|---|
Purified anti-Myelin Basic Protein | SMI 94 | IHC-P,WB,ICC,IHC-F |
Alexa Fluor® 488 anti-Myelin Basic Protein | SMI 94 | IHC-P |
Alexa Fluor® 594 anti-Myelin Basic Protein | SMI 94 | IHC-P |
Spark YG™ 570 anti-Myelin Basic Protein | SMI 94 | IHC-P |
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Compare Data Across All Formats
This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.
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Purified anti-Myelin Basic Protein
IHC staining of purified anti-Myelin Basic Protein antibody ... IHC staining of purified anti-Myelin Basic Protein antibody ... IHC staining of purified anti-Myelin Basic Protein antibody ... Western blot of purified anti-Myelin Basic Protein antibody ... -
Alexa Fluor® 488 anti-Myelin Basic Protein
IHC staining of Alexa Fluor® 488 anti-Myelin Basic Protein a... IHC staining of Alexa Fluor® 488 anti-Myelin Basic Protein a... -
Alexa Fluor® 594 anti-Myelin Basic Protein
IHC staining of Alexa Fluor® 594 anti-Myelin Basic Protein a... IHC staining of Alexa Fluor® 594 anti-Myelin Basic Protein a... IHC staining of Alexa Fluor® 594 anti-Myelin Basic Protein a... -
Spark YG™ 570 anti-Myelin Basic Protein
IHC staining of Spark YG™ 570 anti-Myelin Basic Protein anti... IHC staining of Spark YG™ 570 anti-Myelin Basic Protein anti... IHC staining of Spark YG™ 570 anti-Myelin Basic Protein anti...
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